Alphacoronavirus transmissible gastroenteritis virus nsp1 protein suppresses protein translation in mammalian cells and in cell-free HeLa cell extracts but not in rabbit reticulocyte lysate

Cheng Huang, Kumari G. Lokugamage, Janet M. Rozovics, Krishna Narayanan, Bert L. Semler, Shinji Makino

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The nsp1 protein of transmissible gastroenteritis virus (TGEV), an alphacoronavirus, efficiently suppressed protein synthesis in mammalian cells. Unlike the nsp1 protein of severe acute respiratory syndrome coronavirus, a betacoronavirus, the TGEV nsp1 protein was unable to bind 40S ribosomal subunits or promote host mRNA degradation. TGEV nsp1 also suppressed protein translation in cell-free HeLa cell extract; however, it did not affect translation in rabbit reticulocyte lysate (RRL). Our data suggested that HeLa cell extracts and cultured host cells, but not RRL, contain a host factor(s) that is essential for TGEV nsp1-induced translational suppression.

Original languageEnglish (US)
Pages (from-to)638-643
Number of pages6
JournalJournal of Virology
Volume85
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Alphacoronavirus
Transmissible gastroenteritis virus
reticulocytes
Reticulocytes
Protein Biosynthesis
Cell Extracts
HeLa Cells
translation (genetics)
rabbits
Rabbits
extracts
Proteins
Betacoronavirus
proteins
Eukaryotic Small Ribosome Subunits
cells
Severe Acute Respiratory Syndrome
Coronavirus
RNA Stability
Cultured Cells

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Alphacoronavirus transmissible gastroenteritis virus nsp1 protein suppresses protein translation in mammalian cells and in cell-free HeLa cell extracts but not in rabbit reticulocyte lysate. / Huang, Cheng; Lokugamage, Kumari G.; Rozovics, Janet M.; Narayanan, Krishna; Semler, Bert L.; Makino, Shinji.

In: Journal of Virology, Vol. 85, No. 1, 01.2011, p. 638-643.

Research output: Contribution to journalArticle

@article{0bc07daa43a545fabb89fa46b90e0072,
title = "Alphacoronavirus transmissible gastroenteritis virus nsp1 protein suppresses protein translation in mammalian cells and in cell-free HeLa cell extracts but not in rabbit reticulocyte lysate",
abstract = "The nsp1 protein of transmissible gastroenteritis virus (TGEV), an alphacoronavirus, efficiently suppressed protein synthesis in mammalian cells. Unlike the nsp1 protein of severe acute respiratory syndrome coronavirus, a betacoronavirus, the TGEV nsp1 protein was unable to bind 40S ribosomal subunits or promote host mRNA degradation. TGEV nsp1 also suppressed protein translation in cell-free HeLa cell extract; however, it did not affect translation in rabbit reticulocyte lysate (RRL). Our data suggested that HeLa cell extracts and cultured host cells, but not RRL, contain a host factor(s) that is essential for TGEV nsp1-induced translational suppression.",
author = "Cheng Huang and Lokugamage, {Kumari G.} and Rozovics, {Janet M.} and Krishna Narayanan and Semler, {Bert L.} and Shinji Makino",
year = "2011",
month = "1",
doi = "10.1128/JVI.01806-10",
language = "English (US)",
volume = "85",
pages = "638--643",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Alphacoronavirus transmissible gastroenteritis virus nsp1 protein suppresses protein translation in mammalian cells and in cell-free HeLa cell extracts but not in rabbit reticulocyte lysate

AU - Huang, Cheng

AU - Lokugamage, Kumari G.

AU - Rozovics, Janet M.

AU - Narayanan, Krishna

AU - Semler, Bert L.

AU - Makino, Shinji

PY - 2011/1

Y1 - 2011/1

N2 - The nsp1 protein of transmissible gastroenteritis virus (TGEV), an alphacoronavirus, efficiently suppressed protein synthesis in mammalian cells. Unlike the nsp1 protein of severe acute respiratory syndrome coronavirus, a betacoronavirus, the TGEV nsp1 protein was unable to bind 40S ribosomal subunits or promote host mRNA degradation. TGEV nsp1 also suppressed protein translation in cell-free HeLa cell extract; however, it did not affect translation in rabbit reticulocyte lysate (RRL). Our data suggested that HeLa cell extracts and cultured host cells, but not RRL, contain a host factor(s) that is essential for TGEV nsp1-induced translational suppression.

AB - The nsp1 protein of transmissible gastroenteritis virus (TGEV), an alphacoronavirus, efficiently suppressed protein synthesis in mammalian cells. Unlike the nsp1 protein of severe acute respiratory syndrome coronavirus, a betacoronavirus, the TGEV nsp1 protein was unable to bind 40S ribosomal subunits or promote host mRNA degradation. TGEV nsp1 also suppressed protein translation in cell-free HeLa cell extract; however, it did not affect translation in rabbit reticulocyte lysate (RRL). Our data suggested that HeLa cell extracts and cultured host cells, but not RRL, contain a host factor(s) that is essential for TGEV nsp1-induced translational suppression.

UR - http://www.scopus.com/inward/record.url?scp=78650074159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650074159&partnerID=8YFLogxK

U2 - 10.1128/JVI.01806-10

DO - 10.1128/JVI.01806-10

M3 - Article

VL - 85

SP - 638

EP - 643

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -