Alteration in the coding potential and expression of H-ras in human cytomegalovirus-transformed cells

Istvan Boldogh, E. S. Huang, P. Rady, I. Arany, S. Tyring, T. Albrecht

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

CMV-transformed cell lines demonstrated a greater level of H-ras RNA (7.5- to 9.5-fold) relative to the level for H-ras in parental cells. Nuclear run off assays showed that the RNA levels for the H-ras gene were regulated at the level of transcriptional initiation. The increased RNA levels for H-ras correlated with the level of p21(ras)Val-12 in transformed cells, while p21(ras)Val-12 was below the level of detection in nontransformed cells using Western blot analysis. In addition, an activating mutation was identified in both alleles of the first exon, codon 12 of H-ras resulting in a G:C to T:A transversion in all transformed cell lines examined in this study. These results suggest that the mutated H-ras may be one of the components by which an oncogenic phenotype is maintained in these CMV-transformed cells.

Original languageEnglish (US)
Pages (from-to)321-329
Number of pages9
JournalIntervirology
Volume37
Issue number6
StatePublished - 1994

Fingerprint

Cytomegalovirus
Proto-Oncogene Proteins p21(ras)
Transformed Cell Line
RNA
ras Genes
Codon
Exons
Western Blotting
Alleles
Phenotype
Mutation

Keywords

  • Cytomegalovirus
  • H-ras
  • Mutation
  • Transformation

ASJC Scopus subject areas

  • Virology

Cite this

Boldogh, I., Huang, E. S., Rady, P., Arany, I., Tyring, S., & Albrecht, T. (1994). Alteration in the coding potential and expression of H-ras in human cytomegalovirus-transformed cells. Intervirology, 37(6), 321-329.

Alteration in the coding potential and expression of H-ras in human cytomegalovirus-transformed cells. / Boldogh, Istvan; Huang, E. S.; Rady, P.; Arany, I.; Tyring, S.; Albrecht, T.

In: Intervirology, Vol. 37, No. 6, 1994, p. 321-329.

Research output: Contribution to journalArticle

Boldogh, I, Huang, ES, Rady, P, Arany, I, Tyring, S & Albrecht, T 1994, 'Alteration in the coding potential and expression of H-ras in human cytomegalovirus-transformed cells', Intervirology, vol. 37, no. 6, pp. 321-329.
Boldogh, Istvan ; Huang, E. S. ; Rady, P. ; Arany, I. ; Tyring, S. ; Albrecht, T. / Alteration in the coding potential and expression of H-ras in human cytomegalovirus-transformed cells. In: Intervirology. 1994 ; Vol. 37, No. 6. pp. 321-329.
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