Alteration of clinical outcome and histopathology of yellow fever virus infection in a hamster model by previous infection with heterologous flaviviruses

Shu Yuan Xiao, Hilda Guzman, Amelia P A Travassos Da Rosa, Hong Bing Zhu, Robert B. Tesh

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Using a recently described hamster model of severe yellow fever (YF), we examined the hypothesis that prior infection with heterologous flaviviruses protects against severe or fatal YF. Hamsters were singly or sequentially infected with Japanese encephalitis, St. Louis encephalitis, West Nile, and/or dengue-1 viruses, and then challenged with a virulent strain of yellow fever virus (YFV). In contrast to control (naive) hamsters, many of which appeared clinically ill or died after YFV infection, the flavivirus-immune animals remained asymptomatic. The flavivirus-immune hamsters also had a reduced viremia and lower serum levels of alanine aminotransferase and total bilirubin, compared with naive hamsters, following YFV infection. Histologically, livers of animals in the flavivirus-immune and control groups showed comparable levels of multifocal necrapoptosis. However, steatosis was not observed in the flavivirus-immune animals, whereas naive hamsters developed extensive microvesicular steatosis in the liver following YFV infection. These findings suggest that hepatocytic steatosis is an adverse microscopic feature associated with severe disease in YFV infection. Our experimental results support earlier anecdotal reports that prior exposure of humans to heterologous flaviviruses reduces subsequent risk of fatal YFV infection.

Original languageEnglish
Pages (from-to)695-703
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume68
Issue number6
StatePublished - Jun 2003

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Yellow fever virus
Flavivirus
Virus Diseases
Cricetinae
Infection
Yellow Fever
St. Louis Encephalitis
Japanese Encephalitis
Dengue Virus
Viremia
Fatty Liver
Alanine Transaminase
Bilirubin
Control Groups
Liver
Serum

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Alteration of clinical outcome and histopathology of yellow fever virus infection in a hamster model by previous infection with heterologous flaviviruses. / Xiao, Shu Yuan; Guzman, Hilda; Travassos Da Rosa, Amelia P A; Zhu, Hong Bing; Tesh, Robert B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 68, No. 6, 06.2003, p. 695-703.

Research output: Contribution to journalArticle

Xiao, Shu Yuan ; Guzman, Hilda ; Travassos Da Rosa, Amelia P A ; Zhu, Hong Bing ; Tesh, Robert B. / Alteration of clinical outcome and histopathology of yellow fever virus infection in a hamster model by previous infection with heterologous flaviviruses. In: American Journal of Tropical Medicine and Hygiene. 2003 ; Vol. 68, No. 6. pp. 695-703.
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