Alterations in leukocyte adhesion molecule expression after burn injury

F. E. Nwariaku, W. J. Mileski, E. Lightfoot, P. J. Sikes, P. E. Lipsky

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Objective: To determine if thermal injury alters the expression of leukocyte adhesion molecules. Design: This is a controlled experimental animal study. Materials and Methods: Partial thickness burns were created on the backs of New Zealand White rabbits. At 30 minutes, 2 hours, 4 hours, and 24 hours after burn, skin was harvested for immunohistochemistry. Monoclonal antibodies were used to study changes in intercellular adhesion molecule 1 (ICAM-1), E-selectin, and leukocyte CD11a. Staining was graded on a scale of 0 to 4. Measurements and Main Results: ICAM-1 was significantly decreased at 24 hours after burn (p < 0.007, Wilcoxon signed rank test). CD11a was increased at 30 minutes (p < 0.02), 2 hours (p < 0.02), and 24 hours (p < 0.006). E-selectin was increased at 2 hours (p < 0.03). Conclusion: Thermal injury alters the expression of leukocyte adhesion molecules.

Original languageEnglish (US)
Pages (from-to)285-288
Number of pages4
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume39
Issue number2
DOIs
StatePublished - Sep 12 1995
Externally publishedYes

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Keywords

  • Burns
  • Inflammation
  • Injury
  • Integrins
  • Intercellular adhesion molecule (ICAM)
  • Neutrophil
  • Selectins

ASJC Scopus subject areas

  • Surgery

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