Alterations in leukocyte adhesion molecule expression after burn injury

F. E. Nwariaku, William Mileski, E. Lightfoot, P. J. Sikes, P. E. Lipsky

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: To determine if thermal injury alters the expression of leukocyte adhesion molecules. Design: This is a controlled experimental animal study. Materials and Methods: Partial thickness burns were created on the backs of New Zealand White rabbits. At 30 minutes, 2 hours, 4 hours, and 24 hours after burn, skin was harvested for immunohistochemistry. Monoclonal antibodies were used to study changes in intercellular adhesion molecule 1 (ICAM-1), E-selectin, and leukocyte CD11a. Staining was graded on a scale of 0 to 4. Measurements and Main Results: ICAM-1 was significantly decreased at 24 hours after burn (p < 0.007, Wilcoxon signed rank test). CD11a was increased at 30 minutes (p < 0.02), 2 hours (p < 0.02), and 24 hours (p < 0.006). E-selectin was increased at 2 hours (p < 0.03). Conclusion: Thermal injury alters the expression of leukocyte adhesion molecules.

Original languageEnglish
Pages (from-to)285-288
Number of pages4
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume39
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

E-Selectin
Cell Adhesion Molecules
Intercellular Adhesion Molecule-1
Hot Temperature
Wounds and Injuries
Nonparametric Statistics
Burns
Leukocytes
Immunohistochemistry
Monoclonal Antibodies
Staining and Labeling
Rabbits
Skin

Keywords

  • Burns
  • Inflammation
  • Injury
  • Integrins
  • Intercellular adhesion molecule (ICAM)
  • Neutrophil
  • Selectins

ASJC Scopus subject areas

  • Surgery

Cite this

Alterations in leukocyte adhesion molecule expression after burn injury. / Nwariaku, F. E.; Mileski, William; Lightfoot, E.; Sikes, P. J.; Lipsky, P. E.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 39, No. 2, 1995, p. 285-288.

Research output: Contribution to journalArticle

Nwariaku, F. E. ; Mileski, William ; Lightfoot, E. ; Sikes, P. J. ; Lipsky, P. E. / Alterations in leukocyte adhesion molecule expression after burn injury. In: Journal of Trauma - Injury, Infection and Critical Care. 1995 ; Vol. 39, No. 2. pp. 285-288.
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