Alterations in nitric oxide and cytokine production with airway inflammation in the absence of IL-10

Bill Ameredes, Ruben Zamora, Jigme M. Sethi, He Liang Liu, Lauryn K. Kobut, Amber L. Gligonic, Augustine M K Choi, William Calhoun

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

IL-10 is an anti-inflammatory cytokine that suppresses NO synthase (NOS) and production of NO; its lack may promote NO production and alterations in cytokines modulated by NO with allergic airway inflammation (AI), such as IL-18 and IL-4. Therefore, we induced AI in IL-10 knockout (-/-) and IL-10-sufficient C57BL/6 (C57) mice with inhaled OVA and measured airway NO production, as exhaled NO (ENO) and bronchoalveolar lavage fluid nitrite levels. ENO and nitrite levels were elevated significantly in naive IL-10-/- mice as compared with C57 mice. With AI, E NO and nitrite levels increased in C57 mice and decreased in IL-10-/- mice. IL-18 production fell with both AI and addition of S-nitroso-N-acetyl-D,L-penicillamine (a NO donor) but was not significantly increased by chemical NOS inhibition by L-N5-(1-iminoethyl)- ornithine. IL-4 AI was increased significantly (up to 10-fold greater) in the absence of IL-10 but was reduced significantly with chemical inhibition of NOS. Airway responsiveness was lower in IL-10-/- mice and was associated with alteration in production of NO and IL-4. Thus, IL-4 production was increased, and likely decreased NO production, in a way not predicted by the absence of IL-10. Inhibition of IL-4 production, with inhibition of NOS in the absence of IL-10, demonstrated the importance of a NO and IL-4 feedback mechanism regulating this interaction.

Original languageEnglish (US)
Pages (from-to)1206-1213
Number of pages8
JournalJournal of Immunology
Volume175
Issue number2
StatePublished - Jul 15 2005
Externally publishedYes

Fingerprint

Interleukin-10
Nitric Oxide
Cytokines
Inflammation
Interleukin-4
Nitric Oxide Synthase
Nitrites
Inbred C57BL Mouse
Interleukin-18
Penicillamine
Bronchoalveolar Lavage Fluid
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Immunology

Cite this

Ameredes, B., Zamora, R., Sethi, J. M., Liu, H. L., Kobut, L. K., Gligonic, A. L., ... Calhoun, W. (2005). Alterations in nitric oxide and cytokine production with airway inflammation in the absence of IL-10. Journal of Immunology, 175(2), 1206-1213.

Alterations in nitric oxide and cytokine production with airway inflammation in the absence of IL-10. / Ameredes, Bill; Zamora, Ruben; Sethi, Jigme M.; Liu, He Liang; Kobut, Lauryn K.; Gligonic, Amber L.; Choi, Augustine M K; Calhoun, William.

In: Journal of Immunology, Vol. 175, No. 2, 15.07.2005, p. 1206-1213.

Research output: Contribution to journalArticle

Ameredes, B, Zamora, R, Sethi, JM, Liu, HL, Kobut, LK, Gligonic, AL, Choi, AMK & Calhoun, W 2005, 'Alterations in nitric oxide and cytokine production with airway inflammation in the absence of IL-10', Journal of Immunology, vol. 175, no. 2, pp. 1206-1213.
Ameredes B, Zamora R, Sethi JM, Liu HL, Kobut LK, Gligonic AL et al. Alterations in nitric oxide and cytokine production with airway inflammation in the absence of IL-10. Journal of Immunology. 2005 Jul 15;175(2):1206-1213.
Ameredes, Bill ; Zamora, Ruben ; Sethi, Jigme M. ; Liu, He Liang ; Kobut, Lauryn K. ; Gligonic, Amber L. ; Choi, Augustine M K ; Calhoun, William. / Alterations in nitric oxide and cytokine production with airway inflammation in the absence of IL-10. In: Journal of Immunology. 2005 ; Vol. 175, No. 2. pp. 1206-1213.
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