Alterations in nitric oxide and cytokine production with airway inflammation in the absence of IL-10

Bill T. Ameredes, Ruben Zamora, Jigme M. Sethi, He Liang Liu, Lauryn K. Kobut, Amber L. Gligonic, Augustine M.K. Choi, William J. Calhoun

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


IL-10 is an anti-inflammatory cytokine that suppresses NO synthase (NOS) and production of NO; its lack may promote NO production and alterations in cytokines modulated by NO with allergic airway inflammation (AI), such as IL-18 and IL-4. Therefore, we induced AI in IL-10 knockout (-/-) and IL-10-sufficient C57BL/6 (C57) mice with inhaled OVA and measured airway NO production, as exhaled NO (ENO) and bronchoalveolar lavage fluid nitrite levels. ENO and nitrite levels were elevated significantly in naive IL-10-/- mice as compared with C57 mice. With AI, E NO and nitrite levels increased in C57 mice and decreased in IL-10-/- mice. IL-18 production fell with both AI and addition of S-nitroso-N-acetyl-D,L-penicillamine (a NO donor) but was not significantly increased by chemical NOS inhibition by L-N5-(1-iminoethyl)- ornithine. IL-4 AI was increased significantly (up to 10-fold greater) in the absence of IL-10 but was reduced significantly with chemical inhibition of NOS. Airway responsiveness was lower in IL-10-/- mice and was associated with alteration in production of NO and IL-4. Thus, IL-4 production was increased, and likely decreased NO production, in a way not predicted by the absence of IL-10. Inhibition of IL-4 production, with inhibition of NOS in the absence of IL-10, demonstrated the importance of a NO and IL-4 feedback mechanism regulating this interaction.

Original languageEnglish (US)
Pages (from-to)1206-1213
Number of pages8
JournalJournal of Immunology
Issue number2
StatePublished - Jul 15 2005
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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