Alterations in Resistin Expression after Thermal Injury

Mohan R.K. Dasu, Lavenia LaGrone, William J. Mileski

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Background: Burn injury, it was hypothesized, may induce changes in resistin expression that contribute to postburn metabolic derangements. This study examined resistin gene expression, serum levels of resistin protein, and glucose levels in burned mice. Methods: Ten male Balb-c mice were anesthetized and then given a 30% total burn surface area using heated probes. Burned and sham-burned mice were killed at 2, 4, 24, and 48 hours. The total ribonucleic acid from gonadal fat tissues was isolated for the measurement of resistin gene expression using real-time reverse transcriptase-polymerase chain reaction. Serum levels of resistin, insulin, and glucose were measured. Statistical analysis was performed by two-way analysis of variance using Bonferroni's test to find differences between groups. All p values less than 0.05 were considered significant. Results: Increases in resistin gene expression and serum resistin levels were detected in the burned animals, and these correlated with relative insulin resistance. Conclusion: The findings suggest a potential role for resistin in the pathophysiology of the metabolic response to injury.

Original languageEnglish (US)
Pages (from-to)118-122
Number of pages5
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume56
Issue number1
DOIs
StatePublished - Jan 2004

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Keywords

  • Gene expression
  • Insulin resistance
  • Metabolic derangements
  • Real-time RT-PCR
  • Resistin
  • Thermal injury

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

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