Alterations in splenic lymphocyte subpopulations and increased mortality from sepsis following anesthesia in mice

J. F. Hansbrough, Ramon Zapata Sirvent, E. J. Bartle, J. K. Anderson, L. Elliott, M. A. Mansour, W. H. Carter

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The authors evaluated the potential of a variety of anesthetics in mice to produce subsequent alterations in host defenses. Specific monoclonal antibodies and immunofluorescent microscopy were used to enumerate splenic helper/inducer: suppressor/cytotoxic lymphocyte ratios (HSR), and resistance to bacterial challenge was evaluated by a cecal ligation and puncture (CLP) model. Two hours of anesthesia with the intravenous agents ketamine and pentobarbital and with the inhalational agents isoflurane, enflurane, halothane, and halothane-nitrous oxide, were utilized. All anesthetics produced marked depression in the HSR, measured 24 h postanesthesia (P < 0.05); with all agents, helper T-cell populations were decreased and suppressor populations increased. The HSR remained depressed 72 h postanesthetic, following both ketamine and halothane anesthesia (P< 0.05). A dose-response curve was determined with enflurane; increasing the anesthetic time from 1 to 6 h resulted in progressively greater depression of the HSR 24 h later. Changes in lymphocyte subtypes of similar magnitude were found in mice after burn injury or hind limb crush injury and amputation, whereas simple laparotomy did not produce such changes. Serum corticosterone levels were not elevated 24 h post-anesthetic with enflurane, suggesting that the alterations were not nonspecific stress reactions. Resistance to sepsis was determined by measuring survival for 96 h after CLP. With CLP performed 24 h following 2 h anesthesia, mortality was increased from normal: control mortality 36.3%; ketamine 65.0% (P < 0.023); isoflurane 69.5% (P < 0.006); enflurane 84.2% (P < 0.0002). Anesthesia produces dose-related alterations in splenic helper/inducer and suppressor/cytotoxic lymphocyte populations in mice, which persist or at least 72 h; resistance to subsequent bacterial challenge also is reduced, although the two effects are not proven to be related.

Original languageEnglish (US)
Pages (from-to)267-273
Number of pages7
JournalAnesthesiology
Volume63
Issue number3
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Lymphocyte Subsets
Enflurane
Sepsis
Anesthesia
Lymphocytes
Anesthetics
Mortality
Ketamine
Halothane
Punctures
Ligation
Isoflurane
Population
Intravenous Anesthesia
Nitrous Oxide
Pentobarbital
Corticosterone
Helper-Inducer T-Lymphocytes
Amputation
Laparotomy

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Alterations in splenic lymphocyte subpopulations and increased mortality from sepsis following anesthesia in mice. / Hansbrough, J. F.; Zapata Sirvent, Ramon; Bartle, E. J.; Anderson, J. K.; Elliott, L.; Mansour, M. A.; Carter, W. H.

In: Anesthesiology, Vol. 63, No. 3, 01.01.1985, p. 267-273.

Research output: Contribution to journalArticle

Hansbrough, J. F. ; Zapata Sirvent, Ramon ; Bartle, E. J. ; Anderson, J. K. ; Elliott, L. ; Mansour, M. A. ; Carter, W. H. / Alterations in splenic lymphocyte subpopulations and increased mortality from sepsis following anesthesia in mice. In: Anesthesiology. 1985 ; Vol. 63, No. 3. pp. 267-273.
@article{a8b4df04696f482babf685e746488ca8,
title = "Alterations in splenic lymphocyte subpopulations and increased mortality from sepsis following anesthesia in mice",
abstract = "The authors evaluated the potential of a variety of anesthetics in mice to produce subsequent alterations in host defenses. Specific monoclonal antibodies and immunofluorescent microscopy were used to enumerate splenic helper/inducer: suppressor/cytotoxic lymphocyte ratios (HSR), and resistance to bacterial challenge was evaluated by a cecal ligation and puncture (CLP) model. Two hours of anesthesia with the intravenous agents ketamine and pentobarbital and with the inhalational agents isoflurane, enflurane, halothane, and halothane-nitrous oxide, were utilized. All anesthetics produced marked depression in the HSR, measured 24 h postanesthesia (P < 0.05); with all agents, helper T-cell populations were decreased and suppressor populations increased. The HSR remained depressed 72 h postanesthetic, following both ketamine and halothane anesthesia (P< 0.05). A dose-response curve was determined with enflurane; increasing the anesthetic time from 1 to 6 h resulted in progressively greater depression of the HSR 24 h later. Changes in lymphocyte subtypes of similar magnitude were found in mice after burn injury or hind limb crush injury and amputation, whereas simple laparotomy did not produce such changes. Serum corticosterone levels were not elevated 24 h post-anesthetic with enflurane, suggesting that the alterations were not nonspecific stress reactions. Resistance to sepsis was determined by measuring survival for 96 h after CLP. With CLP performed 24 h following 2 h anesthesia, mortality was increased from normal: control mortality 36.3{\%}; ketamine 65.0{\%} (P < 0.023); isoflurane 69.5{\%} (P < 0.006); enflurane 84.2{\%} (P < 0.0002). Anesthesia produces dose-related alterations in splenic helper/inducer and suppressor/cytotoxic lymphocyte populations in mice, which persist or at least 72 h; resistance to subsequent bacterial challenge also is reduced, although the two effects are not proven to be related.",
author = "Hansbrough, {J. F.} and {Zapata Sirvent}, Ramon and Bartle, {E. J.} and Anderson, {J. K.} and L. Elliott and Mansour, {M. A.} and Carter, {W. H.}",
year = "1985",
month = "1",
day = "1",
doi = "10.1097/00000542-198509000-00005",
language = "English (US)",
volume = "63",
pages = "267--273",
journal = "Anesthesiology",
issn = "0003-3022",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Alterations in splenic lymphocyte subpopulations and increased mortality from sepsis following anesthesia in mice

AU - Hansbrough, J. F.

AU - Zapata Sirvent, Ramon

AU - Bartle, E. J.

AU - Anderson, J. K.

AU - Elliott, L.

AU - Mansour, M. A.

AU - Carter, W. H.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - The authors evaluated the potential of a variety of anesthetics in mice to produce subsequent alterations in host defenses. Specific monoclonal antibodies and immunofluorescent microscopy were used to enumerate splenic helper/inducer: suppressor/cytotoxic lymphocyte ratios (HSR), and resistance to bacterial challenge was evaluated by a cecal ligation and puncture (CLP) model. Two hours of anesthesia with the intravenous agents ketamine and pentobarbital and with the inhalational agents isoflurane, enflurane, halothane, and halothane-nitrous oxide, were utilized. All anesthetics produced marked depression in the HSR, measured 24 h postanesthesia (P < 0.05); with all agents, helper T-cell populations were decreased and suppressor populations increased. The HSR remained depressed 72 h postanesthetic, following both ketamine and halothane anesthesia (P< 0.05). A dose-response curve was determined with enflurane; increasing the anesthetic time from 1 to 6 h resulted in progressively greater depression of the HSR 24 h later. Changes in lymphocyte subtypes of similar magnitude were found in mice after burn injury or hind limb crush injury and amputation, whereas simple laparotomy did not produce such changes. Serum corticosterone levels were not elevated 24 h post-anesthetic with enflurane, suggesting that the alterations were not nonspecific stress reactions. Resistance to sepsis was determined by measuring survival for 96 h after CLP. With CLP performed 24 h following 2 h anesthesia, mortality was increased from normal: control mortality 36.3%; ketamine 65.0% (P < 0.023); isoflurane 69.5% (P < 0.006); enflurane 84.2% (P < 0.0002). Anesthesia produces dose-related alterations in splenic helper/inducer and suppressor/cytotoxic lymphocyte populations in mice, which persist or at least 72 h; resistance to subsequent bacterial challenge also is reduced, although the two effects are not proven to be related.

AB - The authors evaluated the potential of a variety of anesthetics in mice to produce subsequent alterations in host defenses. Specific monoclonal antibodies and immunofluorescent microscopy were used to enumerate splenic helper/inducer: suppressor/cytotoxic lymphocyte ratios (HSR), and resistance to bacterial challenge was evaluated by a cecal ligation and puncture (CLP) model. Two hours of anesthesia with the intravenous agents ketamine and pentobarbital and with the inhalational agents isoflurane, enflurane, halothane, and halothane-nitrous oxide, were utilized. All anesthetics produced marked depression in the HSR, measured 24 h postanesthesia (P < 0.05); with all agents, helper T-cell populations were decreased and suppressor populations increased. The HSR remained depressed 72 h postanesthetic, following both ketamine and halothane anesthesia (P< 0.05). A dose-response curve was determined with enflurane; increasing the anesthetic time from 1 to 6 h resulted in progressively greater depression of the HSR 24 h later. Changes in lymphocyte subtypes of similar magnitude were found in mice after burn injury or hind limb crush injury and amputation, whereas simple laparotomy did not produce such changes. Serum corticosterone levels were not elevated 24 h post-anesthetic with enflurane, suggesting that the alterations were not nonspecific stress reactions. Resistance to sepsis was determined by measuring survival for 96 h after CLP. With CLP performed 24 h following 2 h anesthesia, mortality was increased from normal: control mortality 36.3%; ketamine 65.0% (P < 0.023); isoflurane 69.5% (P < 0.006); enflurane 84.2% (P < 0.0002). Anesthesia produces dose-related alterations in splenic helper/inducer and suppressor/cytotoxic lymphocyte populations in mice, which persist or at least 72 h; resistance to subsequent bacterial challenge also is reduced, although the two effects are not proven to be related.

UR - http://www.scopus.com/inward/record.url?scp=0021933960&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021933960&partnerID=8YFLogxK

U2 - 10.1097/00000542-198509000-00005

DO - 10.1097/00000542-198509000-00005

M3 - Article

C2 - 4025889

AN - SCOPUS:0021933960

VL - 63

SP - 267

EP - 273

JO - Anesthesiology

JF - Anesthesiology

SN - 0003-3022

IS - 3

ER -