Alterations to the maternal circulating proteome after preeclampsia Presented in poster format at the 62nd annual meeting of the Society for Reproductive Investigation, San Francisco, CA, March 25-28, 2015.

Malia Su Qin Murphy, Egle Bytautiene, George Saade, Graeme Neil Smith

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective The long-term maternal cardiovascular and metabolic implications associated with preeclampsia (PE) include risk of hypertension, heart disease, and metabolic syndrome. The objective of this study was to investigate if a recent history of PE was associated with detectable alterations in the circulating maternal proteome. Study Design Six-month postpartum plasma from women with a history of PE (n = 12) and women with uncomplicated obstetrical history (n = 12) were used for analysis. Depleted maternal plasma was analyzed by label-free liquid chromatography-mass spectrometry assay. Identified peptides were searched against the International Protein Index human database version 3.87. Exponentially modified protein abundance indices were used for comparison. Results were analyzed using pathway analysis software. Results A total of 126 eligible peptides were identified for analysis; 3 peptides were differentially expressed in the PE proteome, and an additional 5 peptides were unique to control subjects and 7 to PE subjects. PE peptide profiles were more strongly associated with markers of coagulation and complement activation compared to controls and mapped more significantly to cardiovascular disease (CVD) functions. Stratification of subjects by low (

Original languageEnglish (US)
Pages (from-to)853.e1-853.e9
JournalAmerican Journal of Obstetrics and Gynecology
Volume213
Issue number6
DOIs
StatePublished - 2015

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Posters
San Francisco
Proteome
Pre-Eclampsia
Mothers
Peptides
Complement Activation
Liquid Chromatography
Postpartum Period
Heart Diseases
Mass Spectrometry
Proteins
Cardiovascular Diseases
Software
History
Databases
Hypertension

Keywords

  • cardiovascular risk
  • maternal health
  • preeclampsia
  • proteome

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

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title = "Alterations to the maternal circulating proteome after preeclampsia Presented in poster format at the 62nd annual meeting of the Society for Reproductive Investigation, San Francisco, CA, March 25-28, 2015.",
abstract = "Objective The long-term maternal cardiovascular and metabolic implications associated with preeclampsia (PE) include risk of hypertension, heart disease, and metabolic syndrome. The objective of this study was to investigate if a recent history of PE was associated with detectable alterations in the circulating maternal proteome. Study Design Six-month postpartum plasma from women with a history of PE (n = 12) and women with uncomplicated obstetrical history (n = 12) were used for analysis. Depleted maternal plasma was analyzed by label-free liquid chromatography-mass spectrometry assay. Identified peptides were searched against the International Protein Index human database version 3.87. Exponentially modified protein abundance indices were used for comparison. Results were analyzed using pathway analysis software. Results A total of 126 eligible peptides were identified for analysis; 3 peptides were differentially expressed in the PE proteome, and an additional 5 peptides were unique to control subjects and 7 to PE subjects. PE peptide profiles were more strongly associated with markers of coagulation and complement activation compared to controls and mapped more significantly to cardiovascular disease (CVD) functions. Stratification of subjects by low (",
keywords = "cardiovascular risk, maternal health, preeclampsia, proteome",
author = "Murphy, {Malia Su Qin} and Egle Bytautiene and George Saade and Smith, {Graeme Neil}",
year = "2015",
doi = "10.1016/j.ajog.2015.10.008",
language = "English (US)",
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journal = "American Journal of Obstetrics and Gynecology",
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TY - JOUR

T1 - Alterations to the maternal circulating proteome after preeclampsia Presented in poster format at the 62nd annual meeting of the Society for Reproductive Investigation, San Francisco, CA, March 25-28, 2015.

AU - Murphy, Malia Su Qin

AU - Bytautiene, Egle

AU - Saade, George

AU - Smith, Graeme Neil

PY - 2015

Y1 - 2015

N2 - Objective The long-term maternal cardiovascular and metabolic implications associated with preeclampsia (PE) include risk of hypertension, heart disease, and metabolic syndrome. The objective of this study was to investigate if a recent history of PE was associated with detectable alterations in the circulating maternal proteome. Study Design Six-month postpartum plasma from women with a history of PE (n = 12) and women with uncomplicated obstetrical history (n = 12) were used for analysis. Depleted maternal plasma was analyzed by label-free liquid chromatography-mass spectrometry assay. Identified peptides were searched against the International Protein Index human database version 3.87. Exponentially modified protein abundance indices were used for comparison. Results were analyzed using pathway analysis software. Results A total of 126 eligible peptides were identified for analysis; 3 peptides were differentially expressed in the PE proteome, and an additional 5 peptides were unique to control subjects and 7 to PE subjects. PE peptide profiles were more strongly associated with markers of coagulation and complement activation compared to controls and mapped more significantly to cardiovascular disease (CVD) functions. Stratification of subjects by low (

AB - Objective The long-term maternal cardiovascular and metabolic implications associated with preeclampsia (PE) include risk of hypertension, heart disease, and metabolic syndrome. The objective of this study was to investigate if a recent history of PE was associated with detectable alterations in the circulating maternal proteome. Study Design Six-month postpartum plasma from women with a history of PE (n = 12) and women with uncomplicated obstetrical history (n = 12) were used for analysis. Depleted maternal plasma was analyzed by label-free liquid chromatography-mass spectrometry assay. Identified peptides were searched against the International Protein Index human database version 3.87. Exponentially modified protein abundance indices were used for comparison. Results were analyzed using pathway analysis software. Results A total of 126 eligible peptides were identified for analysis; 3 peptides were differentially expressed in the PE proteome, and an additional 5 peptides were unique to control subjects and 7 to PE subjects. PE peptide profiles were more strongly associated with markers of coagulation and complement activation compared to controls and mapped more significantly to cardiovascular disease (CVD) functions. Stratification of subjects by low (

KW - cardiovascular risk

KW - maternal health

KW - preeclampsia

KW - proteome

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