Altered epithelial cell lineage allocation and global expansion of the crypt epithelial stem cell population are associated with ileitis in SAMP1/YitFc mice

Aida Vidrich, Jenny M. Buzan, Sean Barnes, Brian K. Reuter, Kirstin Skaar, Chibuzo Ilo, Fabio Cominelli, Theresa Pizarro, Steven Cohn

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Crohn's disease is characterized by cycles of mucosal injury and ulceration followed by epithelial regeneration and restoration of normal epithelial function. In this study, we examined whether ileitis in SAMP1/YitFc mice, a recombinant-inbred line that spontaneously develops ileitis resembling human Crohn's disease, was associated with alterations in normal patterns of epithelial differentiation or changes in epithelial regeneration after experimental injury. Increased numbers of Paneth, goblet, and intermediate cells were present focally in the ileum of SAMP1/YitFc mice by 4 weeks of age, before any histological evidence of acute or chronic inflammation. This increase in secretory cells became more pronounced at sites of ileitis with increasing age and inflammation. Additionally, there was mispositioning of Paneth and intermediate cells along the crypt-to-villus unit. A concomitant reduction in the number of absorptive enterocytes was observed. In contrast to the ileal-specific changes in lineage allocation, crypt stem cell numbers began to increase in both the ileum and proximal jejunum at the onset of inflammation in SAMP1/YitFc mice. These data suggest that the alterations in epithelial cell differentiation and increases in the size of the crypt stem cell population observed in SAMP1/YitFc mice are regulated by distinct mechanisms. We speculate that these epithelial alterations may play a role in the pathogenesis of ileitis in this murine model of Crohn's disease.

Original languageEnglish (US)
Pages (from-to)1055-1067
Number of pages13
JournalAmerican Journal of Pathology
Volume166
Issue number4
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

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Ileitis
Cell Lineage
Stem Cells
Epithelial Cells
Crohn Disease
Inflammation
Ileum
Population
Regeneration
Paneth Cells
Goblet Cells
Enterocytes
Wounds and Injuries
Jejunum
Cell Differentiation
Cell Count

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Altered epithelial cell lineage allocation and global expansion of the crypt epithelial stem cell population are associated with ileitis in SAMP1/YitFc mice. / Vidrich, Aida; Buzan, Jenny M.; Barnes, Sean; Reuter, Brian K.; Skaar, Kirstin; Ilo, Chibuzo; Cominelli, Fabio; Pizarro, Theresa; Cohn, Steven.

In: American Journal of Pathology, Vol. 166, No. 4, 01.01.2005, p. 1055-1067.

Research output: Contribution to journalArticle

Vidrich, Aida ; Buzan, Jenny M. ; Barnes, Sean ; Reuter, Brian K. ; Skaar, Kirstin ; Ilo, Chibuzo ; Cominelli, Fabio ; Pizarro, Theresa ; Cohn, Steven. / Altered epithelial cell lineage allocation and global expansion of the crypt epithelial stem cell population are associated with ileitis in SAMP1/YitFc mice. In: American Journal of Pathology. 2005 ; Vol. 166, No. 4. pp. 1055-1067.
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