Altered expression and loss of heterozygosity of the migration and invasion inhibitory protein (MIIP) gene in breast cancer

Fangfang Song, Ling Zhang, Ping Ji, Hong Zheng, Yanrui Zhao, Wei Zhang, Kexin Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Previous studies have characterized the migration and invasion inhibitory protein (MIIP) as a novel putative tumor-suppressor gene that regulates cell migration and invasion as well as the mitotic checkpoint. The MIIP gene is located on chromosome 1p36.22, a common site for deletion in many solid tumors including breast cancer. In the present study, we evaluated MIIP expression and allelic deletion to gain insight into the role of the MIIP gene in breast cancer. MIIP gene mRNA and protein expression was assessed in 86 matched breast cancer and adjacent normal tissues. Loss of heterogeneity (LOH) of the MIIP gene was determined using single-nucleotide polymorphism (SNP) and microsatellite (MS) markers in 149 breast carcinomas and the corresponding normal lymphocytes. The analysis revealed that the expression levels of MIIP mRNA and protein were downregulated in tumor specimens compared to those in corresponding adjacent tissues. Advanced clinical stage and tumor size >2 cm were associated with a decreased MIIP expression level. Twenty-six percent (37/142) of tumors were shown to have LOH at the MIIP locus by MS and SNP markers. Breast cancer patients with LOH at the SNP marker rs2295283 experienced shorter survival time. The attenuated expression and LOH of the MIIP gene may contribute to the poor prognosis of breast cancer, supporting a tumor-suppressing role of MIIP gene in the pathogenesis of this disease.

Original languageEnglish (US)
Pages (from-to)2771-2778
Number of pages8
JournalOncology Reports
Volume33
Issue number6
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

Fingerprint

Loss of Heterozygosity
Breast Neoplasms
Proteins
Single Nucleotide Polymorphism
Neoplasms
Microsatellite Repeats
M Phase Cell Cycle Checkpoints
Messenger RNA
Tumor Suppressor Genes
Cell Movement
Down-Regulation
Chromosomes

Keywords

  • Breast cancer
  • Gene inactivation
  • LOH
  • MIIP
  • Mitosis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Altered expression and loss of heterozygosity of the migration and invasion inhibitory protein (MIIP) gene in breast cancer. / Song, Fangfang; Zhang, Ling; Ji, Ping; Zheng, Hong; Zhao, Yanrui; Zhang, Wei; Chen, Kexin.

In: Oncology Reports, Vol. 33, No. 6, 01.06.2015, p. 2771-2778.

Research output: Contribution to journalArticle

Song, Fangfang ; Zhang, Ling ; Ji, Ping ; Zheng, Hong ; Zhao, Yanrui ; Zhang, Wei ; Chen, Kexin. / Altered expression and loss of heterozygosity of the migration and invasion inhibitory protein (MIIP) gene in breast cancer. In: Oncology Reports. 2015 ; Vol. 33, No. 6. pp. 2771-2778.
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