Altered expression of vascular endothelial growth factor and its receptors in normal saphenous vein and in arterialized and stenotic vein grafts

Kenneth J. Woodside, Joseph J. Naoum, Ronald J. Torry, Xiang Y. Xue, Ann S. Burke, Lyuba Levine, John A. Daller, Glenn C. Hunter

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Background: Myointimal thickening is a major cause saphenous vein graft failure. The prominence of medial and adventitial microvessels in stenotic vein grafts and the known angiogenic effects of vascular endothelial growth factor (VEGF) lead us to investigate the expression of VEGF and its receptors in vein graft arterialization and stenosis. Methods: Normal and arterialized vein graft segments were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) for expression of VEGF-R1 (flt), VEGF-R2 (KDR), and neuropilin-1. The cells expressing VEGF, VEGF-R1, VEGF-R2, and neuropilin-1 were identified in normal, stenotic, and arterialized vein graft segments by immunohistochemistry. Results: Vascular endothelial growth factor, detected in the wall in endothelial cells and adventitial microvessels in normal vein, localized to smooth muscle cells, endothelial cells and adventitial microvessels in arterialized and stenotic vein. VEGF-R1 and VEGF-R2 were expressed infrequently on endothelial cells, macrophages, and smooth muscle cells in arterialized and stenotic vein. Neuropilin-1 was detected in all specimens. RT-PCR demonstrated significantly greater expression of neuropilin-1 in normal vein compared with arterialized vein (P <0.05). Conclusions: The differential expression of VEGF and its receptors in normal, arterialized, and stenotic vein grafts suggests that alterations in VEGF/VEGF-R2/neuropilin-1 interactions may be important determinants of the adaptive response of vein grafts to arterialization.

    Original languageEnglish (US)
    Pages (from-to)561-568
    Number of pages8
    JournalAmerican Journal of Surgery
    Volume186
    Issue number5
    DOIs
    StatePublished - Nov 2003

    Fingerprint

    Vascular Endothelial Growth Factor Receptor
    Saphenous Vein
    Vascular Endothelial Growth Factor A
    Veins
    Neuropilin-1
    Transplants
    Adventitia
    Microvessels
    Endothelial Cells
    Reverse Transcription
    Smooth Muscle Myocytes
    Polymerase Chain Reaction
    Pathologic Constriction
    Immunohistochemistry
    Macrophages

    Keywords

    • Flt-1
    • KDR
    • Neuropilin-1
    • Stenosis
    • Vascular endothelial growth factor
    • Vein graft arterialization

    ASJC Scopus subject areas

    • Surgery

    Cite this

    Altered expression of vascular endothelial growth factor and its receptors in normal saphenous vein and in arterialized and stenotic vein grafts. / Woodside, Kenneth J.; Naoum, Joseph J.; Torry, Ronald J.; Xue, Xiang Y.; Burke, Ann S.; Levine, Lyuba; Daller, John A.; Hunter, Glenn C.

    In: American Journal of Surgery, Vol. 186, No. 5, 11.2003, p. 561-568.

    Research output: Contribution to journalArticle

    Woodside, Kenneth J. ; Naoum, Joseph J. ; Torry, Ronald J. ; Xue, Xiang Y. ; Burke, Ann S. ; Levine, Lyuba ; Daller, John A. ; Hunter, Glenn C. / Altered expression of vascular endothelial growth factor and its receptors in normal saphenous vein and in arterialized and stenotic vein grafts. In: American Journal of Surgery. 2003 ; Vol. 186, No. 5. pp. 561-568.
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    abstract = "Background: Myointimal thickening is a major cause saphenous vein graft failure. The prominence of medial and adventitial microvessels in stenotic vein grafts and the known angiogenic effects of vascular endothelial growth factor (VEGF) lead us to investigate the expression of VEGF and its receptors in vein graft arterialization and stenosis. Methods: Normal and arterialized vein graft segments were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) for expression of VEGF-R1 (flt), VEGF-R2 (KDR), and neuropilin-1. The cells expressing VEGF, VEGF-R1, VEGF-R2, and neuropilin-1 were identified in normal, stenotic, and arterialized vein graft segments by immunohistochemistry. Results: Vascular endothelial growth factor, detected in the wall in endothelial cells and adventitial microvessels in normal vein, localized to smooth muscle cells, endothelial cells and adventitial microvessels in arterialized and stenotic vein. VEGF-R1 and VEGF-R2 were expressed infrequently on endothelial cells, macrophages, and smooth muscle cells in arterialized and stenotic vein. Neuropilin-1 was detected in all specimens. RT-PCR demonstrated significantly greater expression of neuropilin-1 in normal vein compared with arterialized vein (P <0.05). Conclusions: The differential expression of VEGF and its receptors in normal, arterialized, and stenotic vein grafts suggests that alterations in VEGF/VEGF-R2/neuropilin-1 interactions may be important determinants of the adaptive response of vein grafts to arterialization.",
    keywords = "Flt-1, KDR, Neuropilin-1, Stenosis, Vascular endothelial growth factor, Vein graft arterialization",
    author = "Woodside, {Kenneth J.} and Naoum, {Joseph J.} and Torry, {Ronald J.} and Xue, {Xiang Y.} and Burke, {Ann S.} and Lyuba Levine and Daller, {John A.} and Hunter, {Glenn C.}",
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    T1 - Altered expression of vascular endothelial growth factor and its receptors in normal saphenous vein and in arterialized and stenotic vein grafts

    AU - Woodside, Kenneth J.

    AU - Naoum, Joseph J.

    AU - Torry, Ronald J.

    AU - Xue, Xiang Y.

    AU - Burke, Ann S.

    AU - Levine, Lyuba

    AU - Daller, John A.

    AU - Hunter, Glenn C.

    PY - 2003/11

    Y1 - 2003/11

    N2 - Background: Myointimal thickening is a major cause saphenous vein graft failure. The prominence of medial and adventitial microvessels in stenotic vein grafts and the known angiogenic effects of vascular endothelial growth factor (VEGF) lead us to investigate the expression of VEGF and its receptors in vein graft arterialization and stenosis. Methods: Normal and arterialized vein graft segments were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) for expression of VEGF-R1 (flt), VEGF-R2 (KDR), and neuropilin-1. The cells expressing VEGF, VEGF-R1, VEGF-R2, and neuropilin-1 were identified in normal, stenotic, and arterialized vein graft segments by immunohistochemistry. Results: Vascular endothelial growth factor, detected in the wall in endothelial cells and adventitial microvessels in normal vein, localized to smooth muscle cells, endothelial cells and adventitial microvessels in arterialized and stenotic vein. VEGF-R1 and VEGF-R2 were expressed infrequently on endothelial cells, macrophages, and smooth muscle cells in arterialized and stenotic vein. Neuropilin-1 was detected in all specimens. RT-PCR demonstrated significantly greater expression of neuropilin-1 in normal vein compared with arterialized vein (P <0.05). Conclusions: The differential expression of VEGF and its receptors in normal, arterialized, and stenotic vein grafts suggests that alterations in VEGF/VEGF-R2/neuropilin-1 interactions may be important determinants of the adaptive response of vein grafts to arterialization.

    AB - Background: Myointimal thickening is a major cause saphenous vein graft failure. The prominence of medial and adventitial microvessels in stenotic vein grafts and the known angiogenic effects of vascular endothelial growth factor (VEGF) lead us to investigate the expression of VEGF and its receptors in vein graft arterialization and stenosis. Methods: Normal and arterialized vein graft segments were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) for expression of VEGF-R1 (flt), VEGF-R2 (KDR), and neuropilin-1. The cells expressing VEGF, VEGF-R1, VEGF-R2, and neuropilin-1 were identified in normal, stenotic, and arterialized vein graft segments by immunohistochemistry. Results: Vascular endothelial growth factor, detected in the wall in endothelial cells and adventitial microvessels in normal vein, localized to smooth muscle cells, endothelial cells and adventitial microvessels in arterialized and stenotic vein. VEGF-R1 and VEGF-R2 were expressed infrequently on endothelial cells, macrophages, and smooth muscle cells in arterialized and stenotic vein. Neuropilin-1 was detected in all specimens. RT-PCR demonstrated significantly greater expression of neuropilin-1 in normal vein compared with arterialized vein (P <0.05). Conclusions: The differential expression of VEGF and its receptors in normal, arterialized, and stenotic vein grafts suggests that alterations in VEGF/VEGF-R2/neuropilin-1 interactions may be important determinants of the adaptive response of vein grafts to arterialization.

    KW - Flt-1

    KW - KDR

    KW - Neuropilin-1

    KW - Stenosis

    KW - Vascular endothelial growth factor

    KW - Vein graft arterialization

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