Altered interleukin-6 expression in fibroblasts from hypertrophic burn scars

H. Xue, R. L. McCauley, W. Zhang, D. K. Martini

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    Hypertrophic burn scars (HBSs) are characterized by a net accumulation of collagen. The role of growth factors and various cytokines in the pathogenesis of these lesions has not been fully characterized. Interleukin-6 (IL-6) is an important immunoregulatory cytokine that can affect fibroblast function. This study showed increased expression of the IL-6 gene in fibroblasts isolated from patients with HBSs compared with control fibroblasts when the ribonuclease protection assay was used. A subsequent increase in unstimulated and stimulated IL-6 secretion was noted in HBS fibroblasts compared with normal control fibroblasts (unstimulated, 0.3627 ± 0.2017 pg/cell vs 0.0662 ± 0.0786 pg/cell, P = .0054; serum, 0.770 ± 0.523 pg/cell vs 0.233 ± 0.231 pg/cell, P = .0381; serum and interferon-γ, 0.912 ± 0.650 pg/cell and 0.244 ± 0.199 pg/cell, P = .0327). These results are further evidence of the role of inflammatory cytokines in the development of HBSs.

    Original languageEnglish (US)
    Pages (from-to)142-146
    Number of pages5
    JournalJournal of Burn Care and Rehabilitation
    Volume21
    Issue number2
    StatePublished - Mar 2000

    Fingerprint

    Hypertrophic Cicatrix
    Interleukin-6
    Fibroblasts
    Cytokines
    Ribonucleases
    Serum
    Interferons
    Intercellular Signaling Peptides and Proteins
    Collagen
    Genes

    ASJC Scopus subject areas

    • Rehabilitation
    • Surgery
    • Nursing(all)
    • Health Professions(all)
    • Emergency Medicine

    Cite this

    Xue, H., McCauley, R. L., Zhang, W., & Martini, D. K. (2000). Altered interleukin-6 expression in fibroblasts from hypertrophic burn scars. Journal of Burn Care and Rehabilitation, 21(2), 142-146.

    Altered interleukin-6 expression in fibroblasts from hypertrophic burn scars. / Xue, H.; McCauley, R. L.; Zhang, W.; Martini, D. K.

    In: Journal of Burn Care and Rehabilitation, Vol. 21, No. 2, 03.2000, p. 142-146.

    Research output: Contribution to journalArticle

    Xue, H, McCauley, RL, Zhang, W & Martini, DK 2000, 'Altered interleukin-6 expression in fibroblasts from hypertrophic burn scars', Journal of Burn Care and Rehabilitation, vol. 21, no. 2, pp. 142-146.
    Xue, H. ; McCauley, R. L. ; Zhang, W. ; Martini, D. K. / Altered interleukin-6 expression in fibroblasts from hypertrophic burn scars. In: Journal of Burn Care and Rehabilitation. 2000 ; Vol. 21, No. 2. pp. 142-146.
    @article{f5788618fe764314a7b97f6d50948bfa,
    title = "Altered interleukin-6 expression in fibroblasts from hypertrophic burn scars",
    abstract = "Hypertrophic burn scars (HBSs) are characterized by a net accumulation of collagen. The role of growth factors and various cytokines in the pathogenesis of these lesions has not been fully characterized. Interleukin-6 (IL-6) is an important immunoregulatory cytokine that can affect fibroblast function. This study showed increased expression of the IL-6 gene in fibroblasts isolated from patients with HBSs compared with control fibroblasts when the ribonuclease protection assay was used. A subsequent increase in unstimulated and stimulated IL-6 secretion was noted in HBS fibroblasts compared with normal control fibroblasts (unstimulated, 0.3627 ± 0.2017 pg/cell vs 0.0662 ± 0.0786 pg/cell, P = .0054; serum, 0.770 ± 0.523 pg/cell vs 0.233 ± 0.231 pg/cell, P = .0381; serum and interferon-γ, 0.912 ± 0.650 pg/cell and 0.244 ± 0.199 pg/cell, P = .0327). These results are further evidence of the role of inflammatory cytokines in the development of HBSs.",
    author = "H. Xue and McCauley, {R. L.} and W. Zhang and Martini, {D. K.}",
    year = "2000",
    month = "3",
    language = "English (US)",
    volume = "21",
    pages = "142--146",
    journal = "Journal of Burn Care and Research",
    issn = "1559-047X",
    publisher = "Lippincott Williams and Wilkins",
    number = "2",

    }

    TY - JOUR

    T1 - Altered interleukin-6 expression in fibroblasts from hypertrophic burn scars

    AU - Xue, H.

    AU - McCauley, R. L.

    AU - Zhang, W.

    AU - Martini, D. K.

    PY - 2000/3

    Y1 - 2000/3

    N2 - Hypertrophic burn scars (HBSs) are characterized by a net accumulation of collagen. The role of growth factors and various cytokines in the pathogenesis of these lesions has not been fully characterized. Interleukin-6 (IL-6) is an important immunoregulatory cytokine that can affect fibroblast function. This study showed increased expression of the IL-6 gene in fibroblasts isolated from patients with HBSs compared with control fibroblasts when the ribonuclease protection assay was used. A subsequent increase in unstimulated and stimulated IL-6 secretion was noted in HBS fibroblasts compared with normal control fibroblasts (unstimulated, 0.3627 ± 0.2017 pg/cell vs 0.0662 ± 0.0786 pg/cell, P = .0054; serum, 0.770 ± 0.523 pg/cell vs 0.233 ± 0.231 pg/cell, P = .0381; serum and interferon-γ, 0.912 ± 0.650 pg/cell and 0.244 ± 0.199 pg/cell, P = .0327). These results are further evidence of the role of inflammatory cytokines in the development of HBSs.

    AB - Hypertrophic burn scars (HBSs) are characterized by a net accumulation of collagen. The role of growth factors and various cytokines in the pathogenesis of these lesions has not been fully characterized. Interleukin-6 (IL-6) is an important immunoregulatory cytokine that can affect fibroblast function. This study showed increased expression of the IL-6 gene in fibroblasts isolated from patients with HBSs compared with control fibroblasts when the ribonuclease protection assay was used. A subsequent increase in unstimulated and stimulated IL-6 secretion was noted in HBS fibroblasts compared with normal control fibroblasts (unstimulated, 0.3627 ± 0.2017 pg/cell vs 0.0662 ± 0.0786 pg/cell, P = .0054; serum, 0.770 ± 0.523 pg/cell vs 0.233 ± 0.231 pg/cell, P = .0381; serum and interferon-γ, 0.912 ± 0.650 pg/cell and 0.244 ± 0.199 pg/cell, P = .0327). These results are further evidence of the role of inflammatory cytokines in the development of HBSs.

    UR - http://www.scopus.com/inward/record.url?scp=0034024597&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0034024597&partnerID=8YFLogxK

    M3 - Article

    C2 - 10752747

    AN - SCOPUS:0034024597

    VL - 21

    SP - 142

    EP - 146

    JO - Journal of Burn Care and Research

    JF - Journal of Burn Care and Research

    SN - 1559-047X

    IS - 2

    ER -