Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM)

Sanjay Srivastava, Chandra S. Joshi, Prithi P S Sethi, Ashok K. Agrawal, Satish Srivastava, Prahlad K. Seth

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We have studied platelet aggregation and membrane fluidity, along with various biochemical parameters, in 19 non-insulin-dependent diabetes mellitus (NIDDM) patients prior to control of blood sugar, 11 patients after control of blood sugar, and 26 normal subjects. Platelet cholestrol: phospholipid ratio and basal levels of)[Ca+]were comparable between normal and uncontrolled NIDDM. Basal levels of malonaldehyde (MDA) and the levels of [Ca++]i, as well as MDA after the addition of arachidonic acid, were 2.5-fold, 5-fold, and 2.5-fold higher, respectively, in the uncontrolled NIDDM group than normals. Platelet aggregation in response to ADP (0.25 μM), epinephrine (1.25 μM), and arachidonic acid (0.25 mM) was significantly higher in uncontrolled NIDDM than in normals (75%, 40% and 52%, respectively). Platelet fluorescence polarization was also higher in NIDDM patients indicating decreased membrane fluidity in such patients as compared to normals. After control of blood sugar in 11 NIDDM patients, agonist-stimulated platelet aggregation and other biochemical parameters were comparable to normals.

Original languageEnglish (US)
Pages (from-to)451-461
Number of pages11
JournalThrombosis Research
Volume76
Issue number5
DOIs
StatePublished - Dec 1 1994
Externally publishedYes

Fingerprint

Type 2 Diabetes Mellitus
Blood Platelets
Platelet Aggregation
Blood Glucose
Membrane Fluidity
Arachidonic Acid
Fluorescence Polarization
Malondialdehyde
Adenosine Diphosphate
Epinephrine
Phospholipids

Keywords

  • Diabetes
  • Human
  • Malonaldehyde
  • membrane fluidity
  • Platelet aggregation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Srivastava, S., Joshi, C. S., Sethi, P. P. S., Agrawal, A. K., Srivastava, S., & Seth, P. K. (1994). Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM). Thrombosis Research, 76(5), 451-461. https://doi.org/10.1016/0049-3848(95)90177-H

Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM). / Srivastava, Sanjay; Joshi, Chandra S.; Sethi, Prithi P S; Agrawal, Ashok K.; Srivastava, Satish; Seth, Prahlad K.

In: Thrombosis Research, Vol. 76, No. 5, 01.12.1994, p. 451-461.

Research output: Contribution to journalArticle

Srivastava, S, Joshi, CS, Sethi, PPS, Agrawal, AK, Srivastava, S & Seth, PK 1994, 'Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM)', Thrombosis Research, vol. 76, no. 5, pp. 451-461. https://doi.org/10.1016/0049-3848(95)90177-H
Srivastava S, Joshi CS, Sethi PPS, Agrawal AK, Srivastava S, Seth PK. Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM). Thrombosis Research. 1994 Dec 1;76(5):451-461. https://doi.org/10.1016/0049-3848(95)90177-H
Srivastava, Sanjay ; Joshi, Chandra S. ; Sethi, Prithi P S ; Agrawal, Ashok K. ; Srivastava, Satish ; Seth, Prahlad K. / Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM). In: Thrombosis Research. 1994 ; Vol. 76, No. 5. pp. 451-461.
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