Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM)

Sanjay Srivastava; Chandra S. Joshi; Prithi P.S. Sethi; Ashok K. Agrawal; Satish K. Srivastava; Prahlad K. Seth

doi:10.1016/0049-3848(95)90177-H

Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM)

Sanjay Srivastava, Chandra S. Joshi, Prithi P.S. Sethi, Ashok K. Agrawal, Satish K. Srivastava, Prahlad K. Seth

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

We have studied platelet aggregation and membrane fluidity, along with various biochemical parameters, in 19 non-insulin-dependent diabetes mellitus (NIDDM) patients prior to control of blood sugar, 11 patients after control of blood sugar, and 26 normal subjects. Platelet cholestrol: phospholipid ratio and basal levels of)[Ca+]were comparable between normal and uncontrolled NIDDM. Basal levels of malonaldehyde (MDA) and the levels of [Ca⁺⁺]_i, as well as MDA after the addition of arachidonic acid, were 2.5-fold, 5-fold, and 2.5-fold higher, respectively, in the uncontrolled NIDDM group than normals. Platelet aggregation in response to ADP (0.25 μM), epinephrine (1.25 μM), and arachidonic acid (0.25 mM) was significantly higher in uncontrolled NIDDM than in normals (75%, 40% and 52%, respectively). Platelet fluorescence polarization was also higher in NIDDM patients indicating decreased membrane fluidity in such patients as compared to normals. After control of blood sugar in 11 NIDDM patients, agonist-stimulated platelet aggregation and other biochemical parameters were comparable to normals.

Original language	English (US)
Pages (from-to)	451-461
Number of pages	11
Journal	Thrombosis Research
Volume	76
Issue number	5
DOIs	https://doi.org/10.1016/0049-3848(95)90177-H
State	Published - Dec 1 1994
Externally published	Yes

Keywords

Diabetes
Human
Malonaldehyde
Platelet aggregation
membrane fluidity

ASJC Scopus subject areas

Hematology

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10.1016/0049-3848(95)90177-H

Cite this

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title = "Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM)",

abstract = "We have studied platelet aggregation and membrane fluidity, along with various biochemical parameters, in 19 non-insulin-dependent diabetes mellitus (NIDDM) patients prior to control of blood sugar, 11 patients after control of blood sugar, and 26 normal subjects. Platelet cholestrol: phospholipid ratio and basal levels of)[Ca+]were comparable between normal and uncontrolled NIDDM. Basal levels of malonaldehyde (MDA) and the levels of [Ca++]i, as well as MDA after the addition of arachidonic acid, were 2.5-fold, 5-fold, and 2.5-fold higher, respectively, in the uncontrolled NIDDM group than normals. Platelet aggregation in response to ADP (0.25 μM), epinephrine (1.25 μM), and arachidonic acid (0.25 mM) was significantly higher in uncontrolled NIDDM than in normals (75%, 40% and 52%, respectively). Platelet fluorescence polarization was also higher in NIDDM patients indicating decreased membrane fluidity in such patients as compared to normals. After control of blood sugar in 11 NIDDM patients, agonist-stimulated platelet aggregation and other biochemical parameters were comparable to normals.",

keywords = "Diabetes, Human, Malonaldehyde, Platelet aggregation, membrane fluidity",

author = "Sanjay Srivastava and Joshi, {Chandra S.} and Sethi, {Prithi P.S.} and Agrawal, {Ashok K.} and Srivastava, {Satish K.} and Seth, {Prahlad K.}",

note = "Funding Information: The authors are grateful to Dr. R.C. Srimal, Director, I.T.R.C. for his keen interest and support for this work. The financial support by the Indian Council of Medical Research and The National Institutes of Health grant DK 36118 is gratefully acknowledged.",

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AU - Srivastava, Sanjay

AU - Joshi, Chandra S.

AU - Sethi, Prithi P.S.

AU - Agrawal, Ashok K.

AU - Srivastava, Satish K.

AU - Seth, Prahlad K.

N1 - Funding Information: The authors are grateful to Dr. R.C. Srimal, Director, I.T.R.C. for his keen interest and support for this work. The financial support by the Indian Council of Medical Research and The National Institutes of Health grant DK 36118 is gratefully acknowledged.

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Y1 - 1994/12/1

N2 - We have studied platelet aggregation and membrane fluidity, along with various biochemical parameters, in 19 non-insulin-dependent diabetes mellitus (NIDDM) patients prior to control of blood sugar, 11 patients after control of blood sugar, and 26 normal subjects. Platelet cholestrol: phospholipid ratio and basal levels of)[Ca+]were comparable between normal and uncontrolled NIDDM. Basal levels of malonaldehyde (MDA) and the levels of [Ca++]i, as well as MDA after the addition of arachidonic acid, were 2.5-fold, 5-fold, and 2.5-fold higher, respectively, in the uncontrolled NIDDM group than normals. Platelet aggregation in response to ADP (0.25 μM), epinephrine (1.25 μM), and arachidonic acid (0.25 mM) was significantly higher in uncontrolled NIDDM than in normals (75%, 40% and 52%, respectively). Platelet fluorescence polarization was also higher in NIDDM patients indicating decreased membrane fluidity in such patients as compared to normals. After control of blood sugar in 11 NIDDM patients, agonist-stimulated platelet aggregation and other biochemical parameters were comparable to normals.

AB - We have studied platelet aggregation and membrane fluidity, along with various biochemical parameters, in 19 non-insulin-dependent diabetes mellitus (NIDDM) patients prior to control of blood sugar, 11 patients after control of blood sugar, and 26 normal subjects. Platelet cholestrol: phospholipid ratio and basal levels of)[Ca+]were comparable between normal and uncontrolled NIDDM. Basal levels of malonaldehyde (MDA) and the levels of [Ca++]i, as well as MDA after the addition of arachidonic acid, were 2.5-fold, 5-fold, and 2.5-fold higher, respectively, in the uncontrolled NIDDM group than normals. Platelet aggregation in response to ADP (0.25 μM), epinephrine (1.25 μM), and arachidonic acid (0.25 mM) was significantly higher in uncontrolled NIDDM than in normals (75%, 40% and 52%, respectively). Platelet fluorescence polarization was also higher in NIDDM patients indicating decreased membrane fluidity in such patients as compared to normals. After control of blood sugar in 11 NIDDM patients, agonist-stimulated platelet aggregation and other biochemical parameters were comparable to normals.

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Altered platelet functions in non-insulin-dependent diabetes mellitus (NIDDM)

Abstract

Keywords

ASJC Scopus subject areas

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