Altered subcutaneous abdominal adipose tissue lipid synthesis in obese, insulin-resistant humans

Demidmaa Tuvdendorj, Manisha Chandalia, Tumurbaatar Batbayar, Manish Saraf, Carine Beysen, Elizabeth J. Murphy, Nicola Abate

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the variability of subcutaneous abdominal adipose tissue (AT) dynamics in obese subjects with a wide range of insulin sensitivity (IS) and the correlation between these two metabolic measures. Ten obese (BMI 30-40 kg/m2) nondiabetic subjects with (n = 6) and without (n = 4) the metabolic syndrome were studied following a 12-wk 2H2O labeling period. Subcutaneous abdominal AT biopsies were collected. Deuterium incorporation into triglyceride (TG)-glycerol and TG-palmitate were measured by gas chromatography-mass spectrometry for the calculation of fractional TG synthesis (fTG) and fractional de novo lipogenesis (fDNL). Muscle IS and insulin-mediated nonesterified fatty acid (NEFA) suppression (a measure for adipose IS) indexes were derived from the oral glucose tolerance test (OGTT). The ability of subcutaneous abdominal AT to synthesize lipids varied significantly in obese subjects (fTG range 7-28%, fDNL range 1.1-4.6%) with significantly lower values (>35% reduction) for both parameters in obese with the metabolic syndrome. fTG correlated positively with muscle IS (r = 0.64, P = 0.04) and inversely with NEFA suppression during the OGTT (r=0.69, P = 0.03). These results demonstrate a large variability in subcutaneous abdominal AT lipid turnover in obesity. Moreover, a reduced capacity for subcutaneous abdominal AT fat storage is associated with muscle and adipose tissue insulin resistance as well as with the metabolic syndrome, thus identifying a form of obesity at heightened risk for type 2 diabetes and cardiovascular disease.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume305
Issue number8
DOIs
StatePublished - Oct 15 2013

Fingerprint

Abdominal Subcutaneous Fat
Insulin Resistance
Insulin
Lipids
Lipogenesis
Triglycerides
Glucose Tolerance Test
Nonesterified Fatty Acids
Muscles
Obesity
Palmitates
Deuterium
Glycerol
Gas Chromatography-Mass Spectrometry
Type 2 Diabetes Mellitus
Adipose Tissue
Cardiovascular Diseases
Fats
Biopsy

Keywords

  • Adipocyte metabolism
  • Adipose tissue
  • Insulin resistance
  • Lipid metabolism
  • Obesity

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

Cite this

Altered subcutaneous abdominal adipose tissue lipid synthesis in obese, insulin-resistant humans. / Tuvdendorj, Demidmaa; Chandalia, Manisha; Batbayar, Tumurbaatar; Saraf, Manish; Beysen, Carine; Murphy, Elizabeth J.; Abate, Nicola.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 305, No. 8, 15.10.2013.

Research output: Contribution to journalArticle

Tuvdendorj, Demidmaa ; Chandalia, Manisha ; Batbayar, Tumurbaatar ; Saraf, Manish ; Beysen, Carine ; Murphy, Elizabeth J. ; Abate, Nicola. / Altered subcutaneous abdominal adipose tissue lipid synthesis in obese, insulin-resistant humans. In: American Journal of Physiology - Endocrinology and Metabolism. 2013 ; Vol. 305, No. 8.
@article{cafbfaaae42843caab9a91d5d1eab159,
title = "Altered subcutaneous abdominal adipose tissue lipid synthesis in obese, insulin-resistant humans",
abstract = "The purpose of this study was to evaluate the variability of subcutaneous abdominal adipose tissue (AT) dynamics in obese subjects with a wide range of insulin sensitivity (IS) and the correlation between these two metabolic measures. Ten obese (BMI 30-40 kg/m2) nondiabetic subjects with (n = 6) and without (n = 4) the metabolic syndrome were studied following a 12-wk 2H2O labeling period. Subcutaneous abdominal AT biopsies were collected. Deuterium incorporation into triglyceride (TG)-glycerol and TG-palmitate were measured by gas chromatography-mass spectrometry for the calculation of fractional TG synthesis (fTG) and fractional de novo lipogenesis (fDNL). Muscle IS and insulin-mediated nonesterified fatty acid (NEFA) suppression (a measure for adipose IS) indexes were derived from the oral glucose tolerance test (OGTT). The ability of subcutaneous abdominal AT to synthesize lipids varied significantly in obese subjects (fTG range 7-28{\%}, fDNL range 1.1-4.6{\%}) with significantly lower values (>35{\%} reduction) for both parameters in obese with the metabolic syndrome. fTG correlated positively with muscle IS (r = 0.64, P = 0.04) and inversely with NEFA suppression during the OGTT (r=0.69, P = 0.03). These results demonstrate a large variability in subcutaneous abdominal AT lipid turnover in obesity. Moreover, a reduced capacity for subcutaneous abdominal AT fat storage is associated with muscle and adipose tissue insulin resistance as well as with the metabolic syndrome, thus identifying a form of obesity at heightened risk for type 2 diabetes and cardiovascular disease.",
keywords = "Adipocyte metabolism, Adipose tissue, Insulin resistance, Lipid metabolism, Obesity",
author = "Demidmaa Tuvdendorj and Manisha Chandalia and Tumurbaatar Batbayar and Manish Saraf and Carine Beysen and Murphy, {Elizabeth J.} and Nicola Abate",
year = "2013",
month = "10",
day = "15",
doi = "10.1152/ajpendo.00194.2013",
language = "English (US)",
volume = "305",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "8",

}

TY - JOUR

T1 - Altered subcutaneous abdominal adipose tissue lipid synthesis in obese, insulin-resistant humans

AU - Tuvdendorj, Demidmaa

AU - Chandalia, Manisha

AU - Batbayar, Tumurbaatar

AU - Saraf, Manish

AU - Beysen, Carine

AU - Murphy, Elizabeth J.

AU - Abate, Nicola

PY - 2013/10/15

Y1 - 2013/10/15

N2 - The purpose of this study was to evaluate the variability of subcutaneous abdominal adipose tissue (AT) dynamics in obese subjects with a wide range of insulin sensitivity (IS) and the correlation between these two metabolic measures. Ten obese (BMI 30-40 kg/m2) nondiabetic subjects with (n = 6) and without (n = 4) the metabolic syndrome were studied following a 12-wk 2H2O labeling period. Subcutaneous abdominal AT biopsies were collected. Deuterium incorporation into triglyceride (TG)-glycerol and TG-palmitate were measured by gas chromatography-mass spectrometry for the calculation of fractional TG synthesis (fTG) and fractional de novo lipogenesis (fDNL). Muscle IS and insulin-mediated nonesterified fatty acid (NEFA) suppression (a measure for adipose IS) indexes were derived from the oral glucose tolerance test (OGTT). The ability of subcutaneous abdominal AT to synthesize lipids varied significantly in obese subjects (fTG range 7-28%, fDNL range 1.1-4.6%) with significantly lower values (>35% reduction) for both parameters in obese with the metabolic syndrome. fTG correlated positively with muscle IS (r = 0.64, P = 0.04) and inversely with NEFA suppression during the OGTT (r=0.69, P = 0.03). These results demonstrate a large variability in subcutaneous abdominal AT lipid turnover in obesity. Moreover, a reduced capacity for subcutaneous abdominal AT fat storage is associated with muscle and adipose tissue insulin resistance as well as with the metabolic syndrome, thus identifying a form of obesity at heightened risk for type 2 diabetes and cardiovascular disease.

AB - The purpose of this study was to evaluate the variability of subcutaneous abdominal adipose tissue (AT) dynamics in obese subjects with a wide range of insulin sensitivity (IS) and the correlation between these two metabolic measures. Ten obese (BMI 30-40 kg/m2) nondiabetic subjects with (n = 6) and without (n = 4) the metabolic syndrome were studied following a 12-wk 2H2O labeling period. Subcutaneous abdominal AT biopsies were collected. Deuterium incorporation into triglyceride (TG)-glycerol and TG-palmitate were measured by gas chromatography-mass spectrometry for the calculation of fractional TG synthesis (fTG) and fractional de novo lipogenesis (fDNL). Muscle IS and insulin-mediated nonesterified fatty acid (NEFA) suppression (a measure for adipose IS) indexes were derived from the oral glucose tolerance test (OGTT). The ability of subcutaneous abdominal AT to synthesize lipids varied significantly in obese subjects (fTG range 7-28%, fDNL range 1.1-4.6%) with significantly lower values (>35% reduction) for both parameters in obese with the metabolic syndrome. fTG correlated positively with muscle IS (r = 0.64, P = 0.04) and inversely with NEFA suppression during the OGTT (r=0.69, P = 0.03). These results demonstrate a large variability in subcutaneous abdominal AT lipid turnover in obesity. Moreover, a reduced capacity for subcutaneous abdominal AT fat storage is associated with muscle and adipose tissue insulin resistance as well as with the metabolic syndrome, thus identifying a form of obesity at heightened risk for type 2 diabetes and cardiovascular disease.

KW - Adipocyte metabolism

KW - Adipose tissue

KW - Insulin resistance

KW - Lipid metabolism

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=84885579074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885579074&partnerID=8YFLogxK

U2 - 10.1152/ajpendo.00194.2013

DO - 10.1152/ajpendo.00194.2013

M3 - Article

VL - 305

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 8

ER -