Amelioration by mercaptoethylguanidine of the vascular and energetic failure in haemorrhagic shock in the anesthetised rat

Basilia Zingarelli, Harry Ischiropoulos, Andrew L. Salzman, Csaba Szabo

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The effects of mercaptoethylguanidine, a dual inhibitor of the inducible nitric oxide (NO) synthase and cyclooxygenase with scavenging effect on peroxynitrite, was studied on the delayed vascular decompensation and cellular energetic failure in a rat model of haemorrhagic shock. Shack was induced by bleeding of the animals to a mean arterial blood pressure of 50 mmHg. At 3 h, animals were resuscitated with Ringers-lactate and monitored for a subsequent 3 h period. In the treated group mercaptoethylguanidine (10 mg/kg/i.v. bolus, followed by 10 mg/kg/i.v. infusion) was administered from the beginning of the resuscitation. Haemorrhagic shock resulted in the upregulation of both the constitutive and the inducible NO synthase, as measured in the lung. In shocked rats mercaptoethylguanidine prevented the increase in plasma nitrite/nitrate and 6-keto-prostaglandin F(1α) levels, ameliorated the decrease in mean arterial blood pressure, and inhibited the development of vascular hyporeactivity of the thoracic aorta ex vivo. A significant nitrotyrosine staining, an indicator of peroxynitrite formation, was found in thoracic aortic rings from shocked animals, which was prevented by mercaptoethylgunnidine treatment. In ex vivo experiments in peritoneal macrophages obtained from shocked rats, treatment with mercaptoethylguanidine prevented the reduction in the intracellular NAD+ content, ameliorated the suppression of mitochondrial respiration and reduced the development of DNA single strand breaks. Our data suggest that mercaptoethylguanidine may be an useful tool for the experimental therapy of haemorrhagic shock.

Original languageEnglish (US)
Pages (from-to)55-65
Number of pages11
JournalEuropean Journal of Pharmacology
Volume338
Issue number1
DOIs
StatePublished - Oct 29 1997
Externally publishedYes

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Hemorrhagic Shock
Blood Vessels
Arterial Pressure
Peroxynitrous Acid
Nitric Oxide Synthase Type II
Single-Stranded DNA Breaks
Investigational Therapies
Peritoneal Macrophages
Prostaglandins F
Prostaglandin-Endoperoxide Synthases
Nitrites
Thoracic Aorta
Resuscitation
NAD
Nitrates
Respiration
Up-Regulation
Thorax
2-mercaptoethylguanidine
Staining and Labeling

Keywords

  • 6-Keto-prostaglandin-1α
  • Haemorrhagic shock
  • Mercaptoalkylguanidine
  • Nitric oxide (NO)
  • Peroxynitrite

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Amelioration by mercaptoethylguanidine of the vascular and energetic failure in haemorrhagic shock in the anesthetised rat. / Zingarelli, Basilia; Ischiropoulos, Harry; Salzman, Andrew L.; Szabo, Csaba.

In: European Journal of Pharmacology, Vol. 338, No. 1, 29.10.1997, p. 55-65.

Research output: Contribution to journalArticle

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