Amiloride inhibits the growth of human colon cancer cells in vitro

J. Y. Koo, D. Parekh, C. M. Townsend, R. Saydjari, B. M. Evers, A. Farre, J. Ishizuka, J. C. Thompson

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Cytoplasmic alkalinization induced by activation of the Na+ H+ antiport plays an essential role in the initiation of cell proliferation. In the present study we examined the effects of amiloride, a specific and reversible inhibitor of Na+ H+ antiporter, on the growth of human colon cancer cells (HT-29). Amiloride (50-800 μm) inhibited the growth of HT-29 cells in a dose-dependent fashion. Forty-three percent inhibition of growth was found at an amiloride concentration of 400 μm after 4 days of treatment. The inhibitory effect of amiloride on growth of HT-29 cells was reversible since removal of amiloride by a media change after 48 h treatment lead to rapid regrowth to control levels. The reversibility of growth inhibition suggests that amiloride is not a non-specific cytotoxin for HT-29 cells. We examined the possible mechanisms for the inhibitory effects of amiloride. Amiloride (400 μM) completely abolished serum-stimulated ODC activity and inhibited difluoromethylornithine (DMFO)-stimulated putrescine uptake by 56%. We conclude that amiloride inhibits the in vitro growth of human colon cancer cells; since ODC-activity and polyamine transport were both inhibited, the inhibitory effects may be mediated in part by polyamine-dependent processes. Amiloride may be a useful agent in the treatment of colon cancer.

Original languageEnglish (US)
Pages (from-to)385-389
Number of pages5
JournalSurgical oncology
Volume1
Issue number6
DOIs
StatePublished - Dec 1992

Keywords

  • amiloride
  • antiport
  • colon cancer
  • difluoromethylornithine
  • ornithine decarboxylase
  • putrescine

ASJC Scopus subject areas

  • Surgery
  • Oncology

Fingerprint Dive into the research topics of 'Amiloride inhibits the growth of human colon cancer cells in vitro'. Together they form a unique fingerprint.

  • Cite this

    Koo, J. Y., Parekh, D., Townsend, C. M., Saydjari, R., Evers, B. M., Farre, A., Ishizuka, J., & Thompson, J. C. (1992). Amiloride inhibits the growth of human colon cancer cells in vitro. Surgical oncology, 1(6), 385-389. https://doi.org/10.1016/0960-7404(92)90040-R