Amino acid availability regulates the effect of hyperinsulinemia on skin protein metabolism in pigs

Demidmaa Tuvdendorj, Elisabet Børsheim, Carwyn P. Sharp, Xiaojun Zhang, Carrie M. Barone, David L. Chinkes, Robert R. Wolfe

Research output: Contribution to journalArticle

Abstract

The effects of amino acid supply and insulin infusion on skin protein kinetics (fractional synthesis rate (FSR), fractional breakdown rate (FBR), and net balance (NB)) in pigs were investigated. Four-month-old pigs were divided into four groups as follows: control, insulin (INS), amino acid (AA), and INS + AA groups based on the nutritional and hormonal conditions. L-[ring-<sup>13</sup>C<inf>6</inf>]Phenylalanine was infused. FBR was estimated from the enrichment ratio of arterial phenylalanine to intracellular free phenylalanine. Plasma INS was increased (p<0.05) in the INS and INS + AA groups. Plasma glucose was maintained by infusion of glucose in the groups receiving INS. The interventions did not change the NB of skin protein. However, the interventions affected the FSR and FBR differently. An infusion of INS significantly increased both FSR and FBR, although AA infusion did not. When an AA infusion was added to the infusion of insulin (INS + AA group), FSR and FBR were both lower when compared with the INS group. Our data demonstrate that in anesthetized pigs INS infusion did not exert an anabolic effect, but rather it increased AA cycling into and out of skin protein. Because co-infusion of AAs with INS ameliorated this effect, it is likely that the increased AA cycling during INS infusion was related to AA supply. Although protein kinetics were affected by both INS and AAs, none of the interventions affected the skin protein deposition. Thus, skin protein content is closely regulated under normal circumstances and is not subject to transient changes in AAs or hormonal concentrations.

Original languageEnglish (US)
Pages (from-to)17776-17783
Number of pages8
JournalJournal of Biological Chemistry
Volume290
Issue number29
DOIs
StatePublished - Jul 17 2015

Fingerprint

Hyperinsulinism
Metabolism
Skin
Swine
Availability
Insulin
Amino Acids
Proteins
Phenylalanine
Plasmas
Anabolic Agents
Glucose
Kinetics

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Tuvdendorj, D., Børsheim, E., Sharp, C. P., Zhang, X., Barone, C. M., Chinkes, D. L., & Wolfe, R. R. (2015). Amino acid availability regulates the effect of hyperinsulinemia on skin protein metabolism in pigs. Journal of Biological Chemistry, 290(29), 17776-17783. https://doi.org/10.1074/jbc.M114.636100

Amino acid availability regulates the effect of hyperinsulinemia on skin protein metabolism in pigs. / Tuvdendorj, Demidmaa; Børsheim, Elisabet; Sharp, Carwyn P.; Zhang, Xiaojun; Barone, Carrie M.; Chinkes, David L.; Wolfe, Robert R.

In: Journal of Biological Chemistry, Vol. 290, No. 29, 17.07.2015, p. 17776-17783.

Research output: Contribution to journalArticle

Tuvdendorj, Demidmaa ; Børsheim, Elisabet ; Sharp, Carwyn P. ; Zhang, Xiaojun ; Barone, Carrie M. ; Chinkes, David L. ; Wolfe, Robert R. / Amino acid availability regulates the effect of hyperinsulinemia on skin protein metabolism in pigs. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 29. pp. 17776-17783.
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