Amino acids at positions 273 and 394 in rabies virus nucleoprotein are important for both evasion of host RIG-I-mediated antiviral response and pathogenicity

Tatsunori Masatani, Naoto Ito, Kenta Shimizu, Yuki Ito, Keisuke Nakagawa, Masako Abe, Satoko Yamaoka, Makoto Sugiyama

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We previously reported that nucleoprotein (N) is related to the different pathogenicities of the virulent rabies virus strain Nishigahara (Ni) and avirulent strain Ni-CE and also that Ni N, but not Ni-CE N, functions to evade retinoic acid-inducible gene I (RIG-I)-mediated innate immunity. There are three amino acid differences between Ni and Ni-CE N (at positions 273, 394 and 395), indicating that one of these mutations or a combination of mutations is important for the pathogenicity and evasion of innate immunity. We generated Ni-CE mutants in which the amino acids in Ni-CE N were replaced with those of Ni in all combinations. Among the mutants, CE(NiN273/394) with mutations at positions 273 and 394 evaded activation of RIG-I-mediated signaling most efficiently and also showed the highest pathogenicity. This correlation reinforces the relation between evasion of host RIG-I-mediated innate immunity and pathogenicity of rabies virus.

Original languageEnglish (US)
Pages (from-to)168-174
Number of pages7
JournalVirus Research
Volume155
Issue number1
DOIs
StatePublished - Jan 2011
Externally publishedYes

Fingerprint

Rabies virus
Nucleoproteins
Tretinoin
Antiviral Agents
Virulence
Innate Immunity
Amino Acids
Mutation
Genes

Keywords

  • Innate immunity
  • Nucleoprotein
  • Pathogenicity
  • Rabies virus
  • Retinoic acid-inducible gene I

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Amino acids at positions 273 and 394 in rabies virus nucleoprotein are important for both evasion of host RIG-I-mediated antiviral response and pathogenicity. / Masatani, Tatsunori; Ito, Naoto; Shimizu, Kenta; Ito, Yuki; Nakagawa, Keisuke; Abe, Masako; Yamaoka, Satoko; Sugiyama, Makoto.

In: Virus Research, Vol. 155, No. 1, 01.2011, p. 168-174.

Research output: Contribution to journalArticle

Masatani, Tatsunori ; Ito, Naoto ; Shimizu, Kenta ; Ito, Yuki ; Nakagawa, Keisuke ; Abe, Masako ; Yamaoka, Satoko ; Sugiyama, Makoto. / Amino acids at positions 273 and 394 in rabies virus nucleoprotein are important for both evasion of host RIG-I-mediated antiviral response and pathogenicity. In: Virus Research. 2011 ; Vol. 155, No. 1. pp. 168-174.
@article{8a4edece05ca43d69fcf17894cfc731d,
title = "Amino acids at positions 273 and 394 in rabies virus nucleoprotein are important for both evasion of host RIG-I-mediated antiviral response and pathogenicity",
abstract = "We previously reported that nucleoprotein (N) is related to the different pathogenicities of the virulent rabies virus strain Nishigahara (Ni) and avirulent strain Ni-CE and also that Ni N, but not Ni-CE N, functions to evade retinoic acid-inducible gene I (RIG-I)-mediated innate immunity. There are three amino acid differences between Ni and Ni-CE N (at positions 273, 394 and 395), indicating that one of these mutations or a combination of mutations is important for the pathogenicity and evasion of innate immunity. We generated Ni-CE mutants in which the amino acids in Ni-CE N were replaced with those of Ni in all combinations. Among the mutants, CE(NiN273/394) with mutations at positions 273 and 394 evaded activation of RIG-I-mediated signaling most efficiently and also showed the highest pathogenicity. This correlation reinforces the relation between evasion of host RIG-I-mediated innate immunity and pathogenicity of rabies virus.",
keywords = "Innate immunity, Nucleoprotein, Pathogenicity, Rabies virus, Retinoic acid-inducible gene I",
author = "Tatsunori Masatani and Naoto Ito and Kenta Shimizu and Yuki Ito and Keisuke Nakagawa and Masako Abe and Satoko Yamaoka and Makoto Sugiyama",
year = "2011",
month = "1",
doi = "10.1016/j.virusres.2010.09.016",
language = "English (US)",
volume = "155",
pages = "168--174",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Amino acids at positions 273 and 394 in rabies virus nucleoprotein are important for both evasion of host RIG-I-mediated antiviral response and pathogenicity

AU - Masatani, Tatsunori

AU - Ito, Naoto

AU - Shimizu, Kenta

AU - Ito, Yuki

AU - Nakagawa, Keisuke

AU - Abe, Masako

AU - Yamaoka, Satoko

AU - Sugiyama, Makoto

PY - 2011/1

Y1 - 2011/1

N2 - We previously reported that nucleoprotein (N) is related to the different pathogenicities of the virulent rabies virus strain Nishigahara (Ni) and avirulent strain Ni-CE and also that Ni N, but not Ni-CE N, functions to evade retinoic acid-inducible gene I (RIG-I)-mediated innate immunity. There are three amino acid differences between Ni and Ni-CE N (at positions 273, 394 and 395), indicating that one of these mutations or a combination of mutations is important for the pathogenicity and evasion of innate immunity. We generated Ni-CE mutants in which the amino acids in Ni-CE N were replaced with those of Ni in all combinations. Among the mutants, CE(NiN273/394) with mutations at positions 273 and 394 evaded activation of RIG-I-mediated signaling most efficiently and also showed the highest pathogenicity. This correlation reinforces the relation between evasion of host RIG-I-mediated innate immunity and pathogenicity of rabies virus.

AB - We previously reported that nucleoprotein (N) is related to the different pathogenicities of the virulent rabies virus strain Nishigahara (Ni) and avirulent strain Ni-CE and also that Ni N, but not Ni-CE N, functions to evade retinoic acid-inducible gene I (RIG-I)-mediated innate immunity. There are three amino acid differences between Ni and Ni-CE N (at positions 273, 394 and 395), indicating that one of these mutations or a combination of mutations is important for the pathogenicity and evasion of innate immunity. We generated Ni-CE mutants in which the amino acids in Ni-CE N were replaced with those of Ni in all combinations. Among the mutants, CE(NiN273/394) with mutations at positions 273 and 394 evaded activation of RIG-I-mediated signaling most efficiently and also showed the highest pathogenicity. This correlation reinforces the relation between evasion of host RIG-I-mediated innate immunity and pathogenicity of rabies virus.

KW - Innate immunity

KW - Nucleoprotein

KW - Pathogenicity

KW - Rabies virus

KW - Retinoic acid-inducible gene I

UR - http://www.scopus.com/inward/record.url?scp=78650532560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650532560&partnerID=8YFLogxK

U2 - 10.1016/j.virusres.2010.09.016

DO - 10.1016/j.virusres.2010.09.016

M3 - Article

VL - 155

SP - 168

EP - 174

JO - Virus Research

JF - Virus Research

SN - 0168-1702

IS - 1

ER -