Amino acids at positions 273 and 394 in rabies virus nucleoprotein are important for both evasion of host RIG-I-mediated antiviral response and pathogenicity

Tatsunori Masatani, Naoto Ito, Kenta Shimizu, Yuki Ito, Keisuke Nakagawa, Masako Abe, Satoko Yamaoka, Makoto Sugiyama

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

We previously reported that nucleoprotein (N) is related to the different pathogenicities of the virulent rabies virus strain Nishigahara (Ni) and avirulent strain Ni-CE and also that Ni N, but not Ni-CE N, functions to evade retinoic acid-inducible gene I (RIG-I)-mediated innate immunity. There are three amino acid differences between Ni and Ni-CE N (at positions 273, 394 and 395), indicating that one of these mutations or a combination of mutations is important for the pathogenicity and evasion of innate immunity. We generated Ni-CE mutants in which the amino acids in Ni-CE N were replaced with those of Ni in all combinations. Among the mutants, CE(NiN273/394) with mutations at positions 273 and 394 evaded activation of RIG-I-mediated signaling most efficiently and also showed the highest pathogenicity. This correlation reinforces the relation between evasion of host RIG-I-mediated innate immunity and pathogenicity of rabies virus.

Original languageEnglish (US)
Pages (from-to)168-174
Number of pages7
JournalVirus Research
Volume155
Issue number1
DOIs
StatePublished - Jan 1 2011

Keywords

  • Innate immunity
  • Nucleoprotein
  • Pathogenicity
  • Rabies virus
  • Retinoic acid-inducible gene I

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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