Amino acids important for DNA recognition by the response regulator OmpR

Eun Rhee Jee, Wanyun Sheng, Leslie K. Morgan, Ryan Nolet, Xiubei Liao, Linda Kenney

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Response regulators undergo regulated phosphorylation and dephosphorylation at conserved aspartic acid residues in bacterial signal transduction systems. OmpR is a winged helix-turn-helix DNA-binding protein that functions as a global regulator in bacteria and is also important in pathogenesis. A detailed mechanistic picture of how OmpR binds to DNA and activates transcription is lacking. We used NMR spectroscopy to solve the solution structure of the C-terminal domain of OmpR (OmpRC) and to analyze the chemical shift changes that occur upon DNA binding. There is little overlap in the interaction surface with residues of PhoB that were reportedly involved in protein/protein interactions in its head-to-tail dimer. Multiple factors account for the lack of overlap. One is that the spacing between the OmpR half-sites is shorter than observed with PhoB, requiring the arrangement of the two OmpR molecules to be different from that of the PhoB dimer on DNA. A second is the demonstration herein that OmpR can bind to its high affinity site as a monomer. As a result, OmpRC appears to be capable of adopting alternative orientations depending on the precise base composition of the binding site, which also contributes to the lack of overlap. In the presence of DNA, chemical shift changes occur in OmpR in the recognition α-helix 3, the loop between β-strand 4 and α-helix 1, and the loop between β-strands 5 and 6. DNA contact residues are Val203 (T), Arg207 (G), and Arg209 (phosphate backbone). Our results suggest that OmpR binds to DNA as a monomer and then forms a symmetric or asymmetric dimer, depending on the binding site. We propose that during activation OmpR binds to DNA and undergoes a conformational change that promotes phosphorylation of the N-terminal receiver domain, the receiver domains dimerize, and then the second monomer binds to DNA. The flexible linker of OmpR enables the second monomer to bind in multiple orientations (head-to-tail and head-to-head), depending on the specific DNA contacts.

Original languageEnglish (US)
Pages (from-to)8664-8677
Number of pages14
JournalJournal of Biological Chemistry
Volume283
Issue number13
DOIs
StatePublished - Mar 28 2008
Externally publishedYes

Fingerprint

Amino Acids
DNA
Monomers
Dimers
Phosphorylation
Chemical shift
Binding Sites
Signal transduction
Base Composition
DNA-Binding Proteins
Transcription
Aspartic Acid
Nuclear magnetic resonance spectroscopy
Signal Transduction
Bacteria
Proteins
Magnetic Resonance Spectroscopy
Demonstrations
Chemical activation
Phosphates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Amino acids important for DNA recognition by the response regulator OmpR. / Jee, Eun Rhee; Sheng, Wanyun; Morgan, Leslie K.; Nolet, Ryan; Liao, Xiubei; Kenney, Linda.

In: Journal of Biological Chemistry, Vol. 283, No. 13, 28.03.2008, p. 8664-8677.

Research output: Contribution to journalArticle

Jee, Eun Rhee ; Sheng, Wanyun ; Morgan, Leslie K. ; Nolet, Ryan ; Liao, Xiubei ; Kenney, Linda. / Amino acids important for DNA recognition by the response regulator OmpR. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 13. pp. 8664-8677.
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