Amniochorion

A source of interleukin-8

S. J. Fortunato, Ramkumar Menon, K. F. Swan

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

PROBLEM: Preterm labor and premature rupture of the membranes are major complications of pregnancy. We have reported the possible role of amniochorionic membrane in the production of inflammatory cytokines and the early onset of labor. This study was conducted to detect the expression of IL-8 mRNA and peptide production in cultured fetal membranes. METHOD: Amniochorionic membranes were collected from women undergoing elective cesarean section at term. Membranes were cultured in an organ explant system and the expression of IL-8 was studied over a 10-day period by RT-PCR and in situ hybridization. IL-8 peptide localization was accomplished using immunocytochemistry. RESULTS: Constitutive expression of IL-8 mRNA in cultured fetal membranes was demonstrated in both amniotic and chorionic leave cells. mRNA and peptide for IL-8 was homogeneously distributed throughout the amniotic and chorionic cells. CONCLUSION: Human amniochorionic membrane is a source of IL-8 mRNA and peptide.

Original languageEnglish (US)
Pages (from-to)156-162
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume34
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Interleukin-8
Extraembryonic Membranes
Messenger RNA
Peptides
Membranes
Labor Onset
Pregnancy Complications
Premature Obstetric Labor
Cesarean Section
In Situ Hybridization
Immunohistochemistry
Cytokines
Polymerase Chain Reaction

Keywords

  • Amniochorion
  • Chorioamnionitis
  • Cytokines
  • IL-8
  • Inflammation
  • Preterm labor

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Amniochorion : A source of interleukin-8. / Fortunato, S. J.; Menon, Ramkumar; Swan, K. F.

In: American Journal of Reproductive Immunology, Vol. 34, No. 3, 1995, p. 156-162.

Research output: Contribution to journalArticle

Fortunato, SJ, Menon, R & Swan, KF 1995, 'Amniochorion: A source of interleukin-8', American Journal of Reproductive Immunology, vol. 34, no. 3, pp. 156-162.
Fortunato, S. J. ; Menon, Ramkumar ; Swan, K. F. / Amniochorion : A source of interleukin-8. In: American Journal of Reproductive Immunology. 1995 ; Vol. 34, No. 3. pp. 156-162.
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