Amnion-epithelial-cell-derived exosomes demonstrate physiologic state of cell under oxidative stress

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

At term, the signals of fetal maturity and feto-placental tissue aging prompt uterine readiness for delivery by transitioning quiescent myometrium to an active stage. It is still unclear how the signals reach the distant myometrium. Exosomes are a specific type of extracellular vesicle (EVs) that transport molecular signals between cells, and are released from a wide range of cells, including the maternal and fetal cells. In this study, we hypothesize that i) exosomes act as carriers of signals in utero-placental compartments and ii) exosomes reflect the physiologic status of the origin cells. The primary aims of this study were to determine exosomal contents in exosomes derived from primary amnion epithelial cells (AEC). We also determined the effect of oxidative stress on AEC derived exosomal cargo contents. AEC were isolated from amniotic membrane obtained from normal, term, not in labor placentae at delivery, and culture under standard conditions. Oxidative stress was induced using cigarette smoke extract for 48 hours. AEC-conditioned media were collected and exosomes isolated by differential centrifugations. Both growth conditions (normal and oxidative stress induced) produced cup shaped exosomes of around 50 nm, expressed exosomes enriched markers, such as CD9, CD63, CD81 and HSC70, embryonic stem cell marker Nanog, and contained similar amounts of cell free AEC DNA. Using confocal microscopy, the colocalization of histone (H) 3, heat shock protein (HSP) 70 and activated form of prosenescence and term parturition associated marker p38 mitogen activated protein kinase (MAPK) (P-p38 MAPK) co-localized with exosome enrich marker CD9. HSP70 and P-p38MAPK were significantly higher in exosomes from AEC grown under oxidative stress conditions than standard conditions (p

Original languageEnglish (US)
Article numbere0157614
JournalPLoS One
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

exosomes
amnion
Exosomes
Amnion
Oxidative stress
Oxidative Stress
epithelial cells
oxidative stress
Epithelial Cells
p38 Mitogen-Activated Protein Kinases
cells
myometrium
Myometrium
mitogen-activated protein kinase
fetoplacental tissues
HSP70 Heat-Shock Proteins
Centrifugation
Confocal microscopy
Conditioned Culture Medium
Stem cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Amnion-epithelial-cell-derived exosomes demonstrate physiologic state of cell under oxidative stress. / Sheller-Miller, Samantha; Papaconstantinou, John; Urrabaz-Garza, Rheanna; Richardson, Lauren; Saade, George; Salomon, Carlos; Menon, Ramkumar.

In: PLoS One, Vol. 11, No. 6, e0157614, 01.06.2016.

Research output: Contribution to journalArticle

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