Amniotic fluid and maternal race influence responsiveness of fetal membranes to bacteria

Morgan R. Peltier, Cayce O. Drobek, Geeta Bhat, George Saade, Stephen J. Fortunato, Ramkumar Menon

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Spontaneous preterm birth (PTB) and preterm prelabor rupture of membranes (pPROM) occur more frequently in African-American women than in other racial groups. This may be due to an enhanced inflammatory response to pathogens associated with the condition. It is also possible that amniotic fluid (AF) has different immunomodulatory properties in African-American women that increase their risk of PTB and pPROM. To test this, we cultured fetal membranes from European-American and African-American women with sterile medium (control), Escherichia coli, Gardnerella vaginalis, Group B streptococci (GBS), Polyporphorans gingivalis, Mycoplasma hominis, Ureaplasma urealyticum or Ureaplasma parvum in the presence and absence of 50% autologous AF. Cytokine concentrations were quantified in the conditioned medium. All bacterial species increased IL-8 production. IL-1β and TNF-α production were stimulated by LPS, E. coli, and G. vaginalis compared with control, but responses to Group B streptococci and P. gingivalis were limited to IL-1β and TNF-α respectively. Genital mycoplasmas stimulated TNF-α and IL-10 but had no effect on IL-1β production. African-Americans had twice the IL-1β response to E. coli as European-Americans (P= 0.031). Conversely, European-Americans produced more IL-8 in response to LPS than African-Americans (P= 0.026). AF had both pro- and anti-inflammatory properties that varied between races and pathogens. These results suggest that the host response to fetal membrane infections is complex and not generalizable. Interventions to prevent PTB and pPROM may need to be customized based on a patient's race, type of bacterial infection and factors in her AF.

Original languageEnglish (US)
Pages (from-to)68-78
Number of pages11
JournalJournal of Reproductive Immunology
Volume96
Issue number1-2
DOIs
StatePublished - Dec 2012

Fingerprint

Extraembryonic Membranes
Amniotic Fluid
African Americans
Interleukin-1
Mothers
Bacteria
Premature Birth
Gardnerella vaginalis
Rupture
Streptococcus agalactiae
Escherichia coli
Interleukin-8
Membranes
Mycoplasma hominis
Ureaplasma
Ureaplasma urealyticum
Mycoplasma
Conditioned Culture Medium
Bacterial Infections
Interleukin-10

Keywords

  • Amniotic fluid
  • Genital mycoplasma
  • Infection
  • Preterm birth
  • Race

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Amniotic fluid and maternal race influence responsiveness of fetal membranes to bacteria. / Peltier, Morgan R.; Drobek, Cayce O.; Bhat, Geeta; Saade, George; Fortunato, Stephen J.; Menon, Ramkumar.

In: Journal of Reproductive Immunology, Vol. 96, No. 1-2, 12.2012, p. 68-78.

Research output: Contribution to journalArticle

Peltier, Morgan R. ; Drobek, Cayce O. ; Bhat, Geeta ; Saade, George ; Fortunato, Stephen J. ; Menon, Ramkumar. / Amniotic fluid and maternal race influence responsiveness of fetal membranes to bacteria. In: Journal of Reproductive Immunology. 2012 ; Vol. 96, No. 1-2. pp. 68-78.
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