Amniotic fluid concentration of collagenase-1 and collagenese-3 are increased in polyhydremnios

Stephen J. Fortunato, Ramkumar Menon, Nasar U. Ahmed, Michelle Bourgeois, Gary A. Dildy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: Polyhydramnios places increased tension on the fetal membranes and should conceptually be associated with an increased need for tissue remodeling to prevent premature rupture of membranes (PROM). Herein we use polyhydramnios as a model to study the association of the collagenase class of matrix metalloproteinases (MMPs) with tissue remodeling during pregnancy. Methods: Amniotic fluids were collected by transabdominal amniocentesis from women with polyhydramnios and from women with normal amniotic fluid volume at second trimester, third trimester and at term. Fluids were assayed for MMP-1 and MMP-13 (collagenase-1 and 3) using ELISA. The concentrations in polyhydramnios were compared with subjects undergoing amniocentesis with normal amniotic fluid volume. Statistical comparisons were made using Scheffe's method for correction in multiple means comparison. P of <0.05 was considered statistically significant. Results: MMP-1 and MMP-13 are present in the amniotic fluid at all stages of pregnancy. The concentrations of these MMPs were significantly elevated in the polyhydramnios samples independent of gestational age. Conclusion: Polyhydramnios is associated with increased amniotic fluid concentrations of MMP-1 and MMP-13 suggesting their role in tissue remodeling.

Original languageEnglish (US)
Pages (from-to)122-125
Number of pages4
JournalJournal of Perinatal Medicine
Volume32
Issue number2
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Polyhydramnios
Amniotic Fluid
Matrix Metalloproteinase 13
Matrix Metalloproteinase 1
Amniocentesis
Matrix Metalloproteinases
Extraembryonic Membranes
Pregnancy
Third Pregnancy Trimester
Second Pregnancy Trimester
Collagenases
Gestational Age
collagenase 1
Rupture
Enzyme-Linked Immunosorbent Assay
Membranes

Keywords

  • Amniotic fluid
  • Collagenases
  • Fetal membranes
  • Polyhydramnios
  • Pregnancy
  • PROM

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Pediatrics, Perinatology, and Child Health

Cite this

Amniotic fluid concentration of collagenase-1 and collagenese-3 are increased in polyhydremnios. / Fortunato, Stephen J.; Menon, Ramkumar; Ahmed, Nasar U.; Bourgeois, Michelle; Dildy, Gary A.

In: Journal of Perinatal Medicine, Vol. 32, No. 2, 2004, p. 122-125.

Research output: Contribution to journalArticle

Fortunato, Stephen J. ; Menon, Ramkumar ; Ahmed, Nasar U. ; Bourgeois, Michelle ; Dildy, Gary A. / Amniotic fluid concentration of collagenase-1 and collagenese-3 are increased in polyhydremnios. In: Journal of Perinatal Medicine. 2004 ; Vol. 32, No. 2. pp. 122-125.
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