Amniotic fluid concentrations of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 during pregnancy and labor

Gregory J. Locksmith, Penny Clark, Patrick Duff, George Saade, Gregory S. Schultz

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

OBJECTIVE: This study was undertaken to determine whether obstetric factors affect the concentrations of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the amniotic fluid. STUDY DESIGN: We prospectively collected amniotic fluid samples from 109 women at various stages of pregnancy and labor and determined matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 concentrations by means of enzyme-linked immunosorbent sandwich assay systems. With multiple regression analysis we evaluated relationships between amniotic fluid matrix metalloproteinase 9 concentration and tissue inhibitor of metalloproteinase 1 concentration and the following factors: gestational age, presence of labor, cervical dilatation, membrane status, presence of clinical chorioamnionitis, and microbial colonization of the amniotic fluid. RESULTS: The detectable presence of amniotic fluid matrix metalloproteinase 9 was independently associated with intra-amniotic infection, labor, cervical dilatation, and spontaneous rupture of membranes. Chorioamnionitis and amniotic fluid matrix metalloproteinase 9 concentrations were correlated with tissue inhibitor of metalloproteinase 1 levels. CONCLUSIONS: Intra-amniotic infection, advanced labor, and rupture of membranes before the onset of labor were independently associated with the presence of matrix metalloproteinase 9 in the amniotic fluid. Both pathologic and physiologic processes appear to produce shifts in the balance between degradation and synthesis of the extracellular matrix.

Original languageEnglish (US)
Pages (from-to)159-164
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume184
Issue number2
DOIs
StatePublished - 2001

Fingerprint

Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 9
Amniotic Fluid
Pregnancy
Chorioamnionitis
First Labor Stage
Membranes
Labor Onset
Spontaneous Rupture
Pathologic Processes
Infection
Gestational Age
Obstetrics
Extracellular Matrix
Rupture
Enzyme-Linked Immunosorbent Assay
Regression Analysis

Keywords

  • Amniotic fluid
  • Intra-amniotic infection
  • Labor
  • Matrix metalloproteinase 9
  • Spontaneous rupture of membranes
  • Tissue inhibitor of metalloproteinase 1

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Amniotic fluid concentrations of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 during pregnancy and labor. / Locksmith, Gregory J.; Clark, Penny; Duff, Patrick; Saade, George; Schultz, Gregory S.

In: American Journal of Obstetrics and Gynecology, Vol. 184, No. 2, 2001, p. 159-164.

Research output: Contribution to journalArticle

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