TY - JOUR
T1 - Amniotic fluid eicosanoids in preterm and term births
T2 - Effects of risk factors for spontaneous preterm labor
AU - Menon, Ramkumar
AU - Fortunato, Stephen J.
AU - Milne, Ginger L.
AU - Brou, Lina
AU - Carnevale, Claudine
AU - Sanchez, Stephanie C.
AU - Hubbard, Leah
AU - Lappas, Martha
AU - Drobek, Cayce Owens
AU - Taylor, Robert N.
PY - 2011/7
Y1 - 2011/7
N2 - Objective: To evaluate amniotic fluid arachidonic acid metabolites using enzymatic and nonenzymatic (lipid peroxidation) pathways in spontaneous preterm birth and term births, and to estimate whether prostanoid concentrations correlate with risk factors (race, cigarette smoking, and microbial invasion of amniotic cavity) associated with preterm birth. Methods: In a case-control study, amniotic fluid was collected at the time of labor or during cesarean delivery. Amniotic fluid samples were subjected to gas chromatography, negative ion chemical ionization, and mass spectrometry for prostaglandin (PG) E 2, PGF2α, and PGD2 and for 6-keto-PGF1α (thromboxane 2 and F2-isoprostane). Primary analysis examined differences between prostanoid concentrations in preterm birth (n=133) compared with term births (n=189). Secondary stratified analyses (by race, cigarette smoking, and microbial invasion of amniotic cavity) compared eicosanoid concentrations in three epidemiological risk factors. Results: Amniotic fluid F2-isoprostane, PGE2, and PGD 2 were significantly higher at term than in preterm birth, whereas PGF2α was higher in preterm birth 6-keto-PGF1α and thromboxane 2 concentrations were not different. Data stratified by race (African American or white) showed no significant disparity among prostanoid concentrations. Regardless of gestational age status, F2-isoprostane was threefold higher in smokers, and other eicosanoids were also higher in smokers compared with nonsmokers. Preterm birth with microbial invasion of amniotic cavity had significantly higher F2-isoprostane compared with preterm birth without microbial invasion of amniotic cavity. Conclusion: Most amniotic fluid eicosanoid concentrations (F2-isoprostane, PGE 2, and PGD2), are higher at term than in preterm births. The only amniotic fluid eicosanoid that is not higher at term is PGF 2α.
AB - Objective: To evaluate amniotic fluid arachidonic acid metabolites using enzymatic and nonenzymatic (lipid peroxidation) pathways in spontaneous preterm birth and term births, and to estimate whether prostanoid concentrations correlate with risk factors (race, cigarette smoking, and microbial invasion of amniotic cavity) associated with preterm birth. Methods: In a case-control study, amniotic fluid was collected at the time of labor or during cesarean delivery. Amniotic fluid samples were subjected to gas chromatography, negative ion chemical ionization, and mass spectrometry for prostaglandin (PG) E 2, PGF2α, and PGD2 and for 6-keto-PGF1α (thromboxane 2 and F2-isoprostane). Primary analysis examined differences between prostanoid concentrations in preterm birth (n=133) compared with term births (n=189). Secondary stratified analyses (by race, cigarette smoking, and microbial invasion of amniotic cavity) compared eicosanoid concentrations in three epidemiological risk factors. Results: Amniotic fluid F2-isoprostane, PGE2, and PGD 2 were significantly higher at term than in preterm birth, whereas PGF2α was higher in preterm birth 6-keto-PGF1α and thromboxane 2 concentrations were not different. Data stratified by race (African American or white) showed no significant disparity among prostanoid concentrations. Regardless of gestational age status, F2-isoprostane was threefold higher in smokers, and other eicosanoids were also higher in smokers compared with nonsmokers. Preterm birth with microbial invasion of amniotic cavity had significantly higher F2-isoprostane compared with preterm birth without microbial invasion of amniotic cavity. Conclusion: Most amniotic fluid eicosanoid concentrations (F2-isoprostane, PGE 2, and PGD2), are higher at term than in preterm births. The only amniotic fluid eicosanoid that is not higher at term is PGF 2α.
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U2 - 10.1097/AOG.0b013e3182204eaa
DO - 10.1097/AOG.0b013e3182204eaa
M3 - Article
C2 - 21691170
AN - SCOPUS:79959676509
SN - 0029-7844
VL - 118
SP - 121
EP - 134
JO - Obstetrics and gynecology
JF - Obstetrics and gynecology
IS - 1
ER -