Amniotic fluid eicosanoids in preterm and term births

Effects of risk factors for spontaneous preterm labor

Ramkumar Menon, Stephen J. Fortunato, Ginger L. Milne, Lina Brou, Claudine Carnevale, Stephanie C. Sanchez, Leah Hubbard, Martha Lappas, Cayce Owens Drobek, Robert N. Taylor

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Objective: To evaluate amniotic fluid arachidonic acid metabolites using enzymatic and nonenzymatic (lipid peroxidation) pathways in spontaneous preterm birth and term births, and to estimate whether prostanoid concentrations correlate with risk factors (race, cigarette smoking, and microbial invasion of amniotic cavity) associated with preterm birth. Methods: In a case-control study, amniotic fluid was collected at the time of labor or during cesarean delivery. Amniotic fluid samples were subjected to gas chromatography, negative ion chemical ionization, and mass spectrometry for prostaglandin (PG) E 2, PGF, and PGD2 and for 6-keto-PGF (thromboxane 2 and F2-isoprostane). Primary analysis examined differences between prostanoid concentrations in preterm birth (n=133) compared with term births (n=189). Secondary stratified analyses (by race, cigarette smoking, and microbial invasion of amniotic cavity) compared eicosanoid concentrations in three epidemiological risk factors. Results: Amniotic fluid F2-isoprostane, PGE2, and PGD 2 were significantly higher at term than in preterm birth, whereas PGF was higher in preterm birth 6-keto-PGF and thromboxane 2 concentrations were not different. Data stratified by race (African American or white) showed no significant disparity among prostanoid concentrations. Regardless of gestational age status, F2-isoprostane was threefold higher in smokers, and other eicosanoids were also higher in smokers compared with nonsmokers. Preterm birth with microbial invasion of amniotic cavity had significantly higher F2-isoprostane compared with preterm birth without microbial invasion of amniotic cavity. Conclusion: Most amniotic fluid eicosanoid concentrations (F2-isoprostane, PGE 2, and PGD2), are higher at term than in preterm births. The only amniotic fluid eicosanoid that is not higher at term is PGF .

Original languageEnglish (US)
Pages (from-to)121-134
Number of pages14
JournalObstetrics and Gynecology
Volume118
Issue number1
DOIs
StatePublished - Jul 2011
Externally publishedYes

Fingerprint

Term Birth
Eicosanoids
Premature Obstetric Labor
Premature Birth
Amniotic Fluid
F2-Isoprostanes
Prostaglandins
Prostaglandin D2
Dinoprost
Thromboxanes
Prostaglandins E
Smoking
Prostaglandins D
Prostaglandins F
Dinoprostone
Arachidonic Acid
African Americans
Gas Chromatography
Lipid Peroxidation
Gestational Age

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Amniotic fluid eicosanoids in preterm and term births : Effects of risk factors for spontaneous preterm labor. / Menon, Ramkumar; Fortunato, Stephen J.; Milne, Ginger L.; Brou, Lina; Carnevale, Claudine; Sanchez, Stephanie C.; Hubbard, Leah; Lappas, Martha; Drobek, Cayce Owens; Taylor, Robert N.

In: Obstetrics and Gynecology, Vol. 118, No. 1, 07.2011, p. 121-134.

Research output: Contribution to journalArticle

Menon, R, Fortunato, SJ, Milne, GL, Brou, L, Carnevale, C, Sanchez, SC, Hubbard, L, Lappas, M, Drobek, CO & Taylor, RN 2011, 'Amniotic fluid eicosanoids in preterm and term births: Effects of risk factors for spontaneous preterm labor', Obstetrics and Gynecology, vol. 118, no. 1, pp. 121-134. https://doi.org/10.1097/AOG.0b013e3182204eaa
Menon, Ramkumar ; Fortunato, Stephen J. ; Milne, Ginger L. ; Brou, Lina ; Carnevale, Claudine ; Sanchez, Stephanie C. ; Hubbard, Leah ; Lappas, Martha ; Drobek, Cayce Owens ; Taylor, Robert N. / Amniotic fluid eicosanoids in preterm and term births : Effects of risk factors for spontaneous preterm labor. In: Obstetrics and Gynecology. 2011 ; Vol. 118, No. 1. pp. 121-134.
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abstract = "Objective: To evaluate amniotic fluid arachidonic acid metabolites using enzymatic and nonenzymatic (lipid peroxidation) pathways in spontaneous preterm birth and term births, and to estimate whether prostanoid concentrations correlate with risk factors (race, cigarette smoking, and microbial invasion of amniotic cavity) associated with preterm birth. Methods: In a case-control study, amniotic fluid was collected at the time of labor or during cesarean delivery. Amniotic fluid samples were subjected to gas chromatography, negative ion chemical ionization, and mass spectrometry for prostaglandin (PG) E 2, PGF2α, and PGD2 and for 6-keto-PGF1α (thromboxane 2 and F2-isoprostane). Primary analysis examined differences between prostanoid concentrations in preterm birth (n=133) compared with term births (n=189). Secondary stratified analyses (by race, cigarette smoking, and microbial invasion of amniotic cavity) compared eicosanoid concentrations in three epidemiological risk factors. Results: Amniotic fluid F2-isoprostane, PGE2, and PGD 2 were significantly higher at term than in preterm birth, whereas PGF2α was higher in preterm birth 6-keto-PGF1α and thromboxane 2 concentrations were not different. Data stratified by race (African American or white) showed no significant disparity among prostanoid concentrations. Regardless of gestational age status, F2-isoprostane was threefold higher in smokers, and other eicosanoids were also higher in smokers compared with nonsmokers. Preterm birth with microbial invasion of amniotic cavity had significantly higher F2-isoprostane compared with preterm birth without microbial invasion of amniotic cavity. Conclusion: Most amniotic fluid eicosanoid concentrations (F2-isoprostane, PGE 2, and PGD2), are higher at term than in preterm births. The only amniotic fluid eicosanoid that is not higher at term is PGF 2α.",
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T2 - Effects of risk factors for spontaneous preterm labor

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AU - Fortunato, Stephen J.

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AU - Brou, Lina

AU - Carnevale, Claudine

AU - Sanchez, Stephanie C.

AU - Hubbard, Leah

AU - Lappas, Martha

AU - Drobek, Cayce Owens

AU - Taylor, Robert N.

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N2 - Objective: To evaluate amniotic fluid arachidonic acid metabolites using enzymatic and nonenzymatic (lipid peroxidation) pathways in spontaneous preterm birth and term births, and to estimate whether prostanoid concentrations correlate with risk factors (race, cigarette smoking, and microbial invasion of amniotic cavity) associated with preterm birth. Methods: In a case-control study, amniotic fluid was collected at the time of labor or during cesarean delivery. Amniotic fluid samples were subjected to gas chromatography, negative ion chemical ionization, and mass spectrometry for prostaglandin (PG) E 2, PGF2α, and PGD2 and for 6-keto-PGF1α (thromboxane 2 and F2-isoprostane). Primary analysis examined differences between prostanoid concentrations in preterm birth (n=133) compared with term births (n=189). Secondary stratified analyses (by race, cigarette smoking, and microbial invasion of amniotic cavity) compared eicosanoid concentrations in three epidemiological risk factors. Results: Amniotic fluid F2-isoprostane, PGE2, and PGD 2 were significantly higher at term than in preterm birth, whereas PGF2α was higher in preterm birth 6-keto-PGF1α and thromboxane 2 concentrations were not different. Data stratified by race (African American or white) showed no significant disparity among prostanoid concentrations. Regardless of gestational age status, F2-isoprostane was threefold higher in smokers, and other eicosanoids were also higher in smokers compared with nonsmokers. Preterm birth with microbial invasion of amniotic cavity had significantly higher F2-isoprostane compared with preterm birth without microbial invasion of amniotic cavity. Conclusion: Most amniotic fluid eicosanoid concentrations (F2-isoprostane, PGE 2, and PGD2), are higher at term than in preterm births. The only amniotic fluid eicosanoid that is not higher at term is PGF 2α.

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