Amniotic fluid prostaglandin E2 in pregnancies complicated by preterm prelabor rupture of the membranes

Ivana Musilova, Ctirad Andrys, Marcela Drahosova, Helena Hornychova, B. Jacobsson, Ramkumar Menon, Piotr Laudanski, Martin Stepan, Tomas Bestvina, Marian Kacerovsky

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Objective: To determine amniotic fluid prostaglandin E2 concentrations in women preterm prelabor rupture of the membranes (PPROM) with respect to microbial invasion of the amniotic cavity (MIAC), intraamniotic inflammation (IAI), microbial-associated IAI, histological chorioamnionitis, and short-term neonatal morbidity. Methods: One hundred forty-five women with singleton pregnancies were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis and were assayed for prostaglandin E2 concentrations by ELISA. IAI was defined as amniotic fluid interleukin-6 >745 pg/mL. Microbial-associated IAI was defined as the presence of both MIAC and IAI. Result: No differences in prostaglandin E2 concentrations were found between women with and without MIAC (p = 0.27). Women with IAI (p = 0.0008) and microbial-associated IAI (p = 0.01) had higher prostaglandin E2 concentrations than women without these complications. Women with histological chorioamnionitis had higher prostaglandin E2 concentrations only in crude analysis (p = 0.02), but not after adjustment for gestational age at sampling (p = 0.10). No associations between amniotic fluid prostaglandin E2 concentrations and the selected conditions of severe neonatal morbidity were found. Conclusions: The intraamniotic inflammatory response either to infectious or to non-infectious stimulus, but not MIAC per se, seems to be a main factor associated with the elevation of the amniotic fluid PGE2 concentrations in women with PPROM.

    Original languageEnglish (US)
    Pages (from-to)1-9
    Number of pages9
    JournalJournal of Maternal-Fetal and Neonatal Medicine
    DOIs
    StateAccepted/In press - Nov 20 2015

    Fingerprint

    Amniotic Fluid
    Dinoprostone
    Rupture
    Inflammation
    Pregnancy
    Membranes
    Chorioamnionitis
    Morbidity
    Amniocentesis
    Gestational Age
    Interleukin-6
    Enzyme-Linked Immunosorbent Assay

    Keywords

    • Infection
    • inflammation
    • placenta
    • preterm delivery

    ASJC Scopus subject areas

    • Pediatrics, Perinatology, and Child Health
    • Obstetrics and Gynecology

    Cite this

    Amniotic fluid prostaglandin E2 in pregnancies complicated by preterm prelabor rupture of the membranes. / Musilova, Ivana; Andrys, Ctirad; Drahosova, Marcela; Hornychova, Helena; Jacobsson, B.; Menon, Ramkumar; Laudanski, Piotr; Stepan, Martin; Bestvina, Tomas; Kacerovsky, Marian.

    In: Journal of Maternal-Fetal and Neonatal Medicine, 20.11.2015, p. 1-9.

    Research output: Contribution to journalArticle

    Musilova, I, Andrys, C, Drahosova, M, Hornychova, H, Jacobsson, B, Menon, R, Laudanski, P, Stepan, M, Bestvina, T & Kacerovsky, M 2015, 'Amniotic fluid prostaglandin E2 in pregnancies complicated by preterm prelabor rupture of the membranes', Journal of Maternal-Fetal and Neonatal Medicine, pp. 1-9. https://doi.org/10.3109/14767058.2015.1112372
    Musilova, Ivana ; Andrys, Ctirad ; Drahosova, Marcela ; Hornychova, Helena ; Jacobsson, B. ; Menon, Ramkumar ; Laudanski, Piotr ; Stepan, Martin ; Bestvina, Tomas ; Kacerovsky, Marian. / Amniotic fluid prostaglandin E2 in pregnancies complicated by preterm prelabor rupture of the membranes. In: Journal of Maternal-Fetal and Neonatal Medicine. 2015 ; pp. 1-9.
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    AU - Musilova, Ivana

    AU - Andrys, Ctirad

    AU - Drahosova, Marcela

    AU - Hornychova, Helena

    AU - Jacobsson, B.

    AU - Menon, Ramkumar

    AU - Laudanski, Piotr

    AU - Stepan, Martin

    AU - Bestvina, Tomas

    AU - Kacerovsky, Marian

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    N2 - Objective: To determine amniotic fluid prostaglandin E2 concentrations in women preterm prelabor rupture of the membranes (PPROM) with respect to microbial invasion of the amniotic cavity (MIAC), intraamniotic inflammation (IAI), microbial-associated IAI, histological chorioamnionitis, and short-term neonatal morbidity. Methods: One hundred forty-five women with singleton pregnancies were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis and were assayed for prostaglandin E2 concentrations by ELISA. IAI was defined as amniotic fluid interleukin-6 >745 pg/mL. Microbial-associated IAI was defined as the presence of both MIAC and IAI. Result: No differences in prostaglandin E2 concentrations were found between women with and without MIAC (p = 0.27). Women with IAI (p = 0.0008) and microbial-associated IAI (p = 0.01) had higher prostaglandin E2 concentrations than women without these complications. Women with histological chorioamnionitis had higher prostaglandin E2 concentrations only in crude analysis (p = 0.02), but not after adjustment for gestational age at sampling (p = 0.10). No associations between amniotic fluid prostaglandin E2 concentrations and the selected conditions of severe neonatal morbidity were found. Conclusions: The intraamniotic inflammatory response either to infectious or to non-infectious stimulus, but not MIAC per se, seems to be a main factor associated with the elevation of the amniotic fluid PGE2 concentrations in women with PPROM.

    AB - Objective: To determine amniotic fluid prostaglandin E2 concentrations in women preterm prelabor rupture of the membranes (PPROM) with respect to microbial invasion of the amniotic cavity (MIAC), intraamniotic inflammation (IAI), microbial-associated IAI, histological chorioamnionitis, and short-term neonatal morbidity. Methods: One hundred forty-five women with singleton pregnancies were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis and were assayed for prostaglandin E2 concentrations by ELISA. IAI was defined as amniotic fluid interleukin-6 >745 pg/mL. Microbial-associated IAI was defined as the presence of both MIAC and IAI. Result: No differences in prostaglandin E2 concentrations were found between women with and without MIAC (p = 0.27). Women with IAI (p = 0.0008) and microbial-associated IAI (p = 0.01) had higher prostaglandin E2 concentrations than women without these complications. Women with histological chorioamnionitis had higher prostaglandin E2 concentrations only in crude analysis (p = 0.02), but not after adjustment for gestational age at sampling (p = 0.10). No associations between amniotic fluid prostaglandin E2 concentrations and the selected conditions of severe neonatal morbidity were found. Conclusions: The intraamniotic inflammatory response either to infectious or to non-infectious stimulus, but not MIAC per se, seems to be a main factor associated with the elevation of the amniotic fluid PGE2 concentrations in women with PPROM.

    KW - Infection

    KW - inflammation

    KW - placenta

    KW - preterm delivery

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