An AAV promoter-driven neuropeptide Y gene delivery system using Sendai virosomes for neurons and rat brain

Ping Wu, C. M. De Fiebre, W. J. Millard, M. A. King, S. Wang, S. O. Bryant, Y. P. Gao, E. J. Martin, E. M. Meyer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An adeno-associated virus (AAV)-derived construct (pJDT95npy) containing rat neuropeptide Y (NPY) cDNA inserted downstream of endogenous AAV promoters was used to investigate AAV-driven NPY expression in post-mitotic neurons in vitro and in the brain. NPY mRNA was expressed in NT2/N and rat brain primary neuronal cultures after transfection. There was a corresponding increase in the number of neurons staining for NPY-like immunoreactivity and an increase in NPY release during depolarization in the primary cultures. Injections of Sendai-virosome encapsulated pJDT95npy into neocortex increased NPY-like immunoreactivity in neurons but not glia indicating that the latter cell type did not have the translational, post-translational or storage capacity to accumulate the peptide. Injections into the rat hypothalamic paraventricular nucleus increased body weight and food intake for 21 days, though NPY-like immunoreactivity remained elevated for at least 50 days. These studies demonstrate that AAV-derived constructs may be useful for delivering genes into post-mitotic neurons, and that Sendai virosomes are effective for delivering these constructs in vivo.

Original languageEnglish (US)
Pages (from-to)246-253
Number of pages8
JournalGene Therapy
Volume3
Issue number3
StatePublished - Mar 1996
Externally publishedYes

Fingerprint

Virosomes
Gene Transfer Techniques
Dependovirus
Neuropeptide Y
Neurons
Brain
Injections
Paraventricular Hypothalamic Nucleus
Neocortex
Neuroglia
Transfection
Complementary DNA
Eating
Body Weight
Staining and Labeling
Messenger RNA
Peptides

Keywords

  • Adeno-associated virus
  • Brain
  • Feeding behavior
  • Virosome
  • Y

ASJC Scopus subject areas

  • Genetics

Cite this

Wu, P., De Fiebre, C. M., Millard, W. J., King, M. A., Wang, S., Bryant, S. O., ... Meyer, E. M. (1996). An AAV promoter-driven neuropeptide Y gene delivery system using Sendai virosomes for neurons and rat brain. Gene Therapy, 3(3), 246-253.

An AAV promoter-driven neuropeptide Y gene delivery system using Sendai virosomes for neurons and rat brain. / Wu, Ping; De Fiebre, C. M.; Millard, W. J.; King, M. A.; Wang, S.; Bryant, S. O.; Gao, Y. P.; Martin, E. J.; Meyer, E. M.

In: Gene Therapy, Vol. 3, No. 3, 03.1996, p. 246-253.

Research output: Contribution to journalArticle

Wu, P, De Fiebre, CM, Millard, WJ, King, MA, Wang, S, Bryant, SO, Gao, YP, Martin, EJ & Meyer, EM 1996, 'An AAV promoter-driven neuropeptide Y gene delivery system using Sendai virosomes for neurons and rat brain', Gene Therapy, vol. 3, no. 3, pp. 246-253.
Wu P, De Fiebre CM, Millard WJ, King MA, Wang S, Bryant SO et al. An AAV promoter-driven neuropeptide Y gene delivery system using Sendai virosomes for neurons and rat brain. Gene Therapy. 1996 Mar;3(3):246-253.
Wu, Ping ; De Fiebre, C. M. ; Millard, W. J. ; King, M. A. ; Wang, S. ; Bryant, S. O. ; Gao, Y. P. ; Martin, E. J. ; Meyer, E. M. / An AAV promoter-driven neuropeptide Y gene delivery system using Sendai virosomes for neurons and rat brain. In: Gene Therapy. 1996 ; Vol. 3, No. 3. pp. 246-253.
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