An animal model for hemolytic disease of the fetus and newborn: I. Alloimmunization techniques

Kenneth J. Moise, L. Scott Rodkey, George Saade, Alfredo Gei, Maurice Duré, Annelle Graham, Carol Creech

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

OBJECTIVE: Our purpose was to establish animal model for hemolytic disease of the fetus and newborn by developing red blood cell alloimmunization techniques in the rabbit. STUDY DESIGN: Twenty-six nonpregnant New Zealand White or Red does underwent blood typing to identify them as homozygous at the HgA or HgF red blood cells antigen locus. Alloimmunization to incompatible red blood cells was attempted through a series of subcutaneous injections using complete then incomplete Freund's adjuvant. RESULTS: Successful induction of an antibody response occurred in 96% of cases. The median response in FF rabbits was 2560 (range 40 to 10,240), whereas the response in AA does was 2560 (range 320 to 20,480). These responses were not statistically different (p - 0.77). Responses were categorized as poor, moderate, or good. No difference was noted between FF and AA does in distribution of the categories of response (p = 0.53). CONCLUSION: Red blood cell alloantibodies in high titer can be induced successfully in the rabbit.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalAmerican journal of obstetrics and gynecology
Volume173
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • Rabbit
  • antibody
  • hemolytic disease of the newborn
  • red blood cell alloimmunization

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Fingerprint

Dive into the research topics of 'An animal model for hemolytic disease of the fetus and newborn: I. Alloimmunization techniques'. Together they form a unique fingerprint.

Cite this