An animal model for hemolytic disease of the fetus or newborn in New Zealand white and New Zealand red rabbits: Newborn effects

Jr Moise K.J., L. S. Rodkey, M. Yared, L. Hudon, George Saade, K. Dorman, A. Graham

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

OBJECTIVE: Our purpose was to study the neonatal effects of red blood cell alloimmunization in a rabbit model. STUDY DESIGN: Eighteen does were alloimmunized to incompatible red blood cells. Does were bred twice, once with a homozygous buck of incompatible blood type and once with a homozygous buck of compatible blood type. Fetal blood sampling was undertaken on day 27 of gestation (term 28 to 31 days). Does were delivered on day 30 and the neonatal pups were anesthetized. Direct cardiac samplings were performed for hemoglobin, reticulocyte count, and direct Coombs' test. Hepatic, splenic, and renal wet weights were measured. RESULTS: Twenty-two pregnancies (12 compatible and 10 incompatible) were studied. Neonatal hemoglobin was higher in the compatible litters (11.1 gm/dL [7.7 to 12.6 gm/dL] vs 4.9 gm/dL [2.1 to 9.1 gm/dL], P < .001), whereas no difference could be detected between the respective reticulocyte counts (34.0/100 red blood cells [27.3 to 36.1/100 red blood cells] vs 32.6/100 red blood cells [26.8 to 43.5/100 red blood cells], P = .55). The direct Coombs' assay was negative in 23 pups from 8 compatible litters and false positive (weakly positive result) in 2 pups of a ninth compatible litter. The Coombs' assay was positive in all 22 incompatible pups tested. Hepatosplenomegaly was noted in affected pups but not in controls. CONCLUSIONS: A disease analogous to human hemolytic disease of the newborn can be induced in the rabbit neonate.

Original languageEnglish (US)
Pages (from-to)1353-1358
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume179
Issue number5
StatePublished - 1998
Externally publishedYes

Fingerprint

Animal Disease Models
New Zealand
Fetus
Erythrocytes
Rabbits
Reticulocyte Count
Hemoglobins
Fetal Erythroblastosis
Coombs Test
Pregnancy
Fetal Blood
Kidney
Weights and Measures
Liver

Keywords

  • Animal model
  • Hemolytic disease of the newborn
  • New Zealand Red rabbit
  • New Zealand White rabbit
  • Rabbit
  • Red blood cell alloimmunization
  • Rhesus disease

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

An animal model for hemolytic disease of the fetus or newborn in New Zealand white and New Zealand red rabbits : Newborn effects. / Moise K.J., Jr; Rodkey, L. S.; Yared, M.; Hudon, L.; Saade, George; Dorman, K.; Graham, A.

In: American Journal of Obstetrics and Gynecology, Vol. 179, No. 5, 1998, p. 1353-1358.

Research output: Contribution to journalArticle

Moise K.J., Jr ; Rodkey, L. S. ; Yared, M. ; Hudon, L. ; Saade, George ; Dorman, K. ; Graham, A. / An animal model for hemolytic disease of the fetus or newborn in New Zealand white and New Zealand red rabbits : Newborn effects. In: American Journal of Obstetrics and Gynecology. 1998 ; Vol. 179, No. 5. pp. 1353-1358.
@article{102d469625924d6b9dbff020fd0b63f5,
title = "An animal model for hemolytic disease of the fetus or newborn in New Zealand white and New Zealand red rabbits: Newborn effects",
abstract = "OBJECTIVE: Our purpose was to study the neonatal effects of red blood cell alloimmunization in a rabbit model. STUDY DESIGN: Eighteen does were alloimmunized to incompatible red blood cells. Does were bred twice, once with a homozygous buck of incompatible blood type and once with a homozygous buck of compatible blood type. Fetal blood sampling was undertaken on day 27 of gestation (term 28 to 31 days). Does were delivered on day 30 and the neonatal pups were anesthetized. Direct cardiac samplings were performed for hemoglobin, reticulocyte count, and direct Coombs' test. Hepatic, splenic, and renal wet weights were measured. RESULTS: Twenty-two pregnancies (12 compatible and 10 incompatible) were studied. Neonatal hemoglobin was higher in the compatible litters (11.1 gm/dL [7.7 to 12.6 gm/dL] vs 4.9 gm/dL [2.1 to 9.1 gm/dL], P < .001), whereas no difference could be detected between the respective reticulocyte counts (34.0/100 red blood cells [27.3 to 36.1/100 red blood cells] vs 32.6/100 red blood cells [26.8 to 43.5/100 red blood cells], P = .55). The direct Coombs' assay was negative in 23 pups from 8 compatible litters and false positive (weakly positive result) in 2 pups of a ninth compatible litter. The Coombs' assay was positive in all 22 incompatible pups tested. Hepatosplenomegaly was noted in affected pups but not in controls. CONCLUSIONS: A disease analogous to human hemolytic disease of the newborn can be induced in the rabbit neonate.",
keywords = "Animal model, Hemolytic disease of the newborn, New Zealand Red rabbit, New Zealand White rabbit, Rabbit, Red blood cell alloimmunization, Rhesus disease",
author = "{Moise K.J.}, Jr and Rodkey, {L. S.} and M. Yared and L. Hudon and George Saade and K. Dorman and A. Graham",
year = "1998",
language = "English (US)",
volume = "179",
pages = "1353--1358",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - An animal model for hemolytic disease of the fetus or newborn in New Zealand white and New Zealand red rabbits

T2 - Newborn effects

AU - Moise K.J., Jr

AU - Rodkey, L. S.

AU - Yared, M.

AU - Hudon, L.

AU - Saade, George

AU - Dorman, K.

AU - Graham, A.

PY - 1998

Y1 - 1998

N2 - OBJECTIVE: Our purpose was to study the neonatal effects of red blood cell alloimmunization in a rabbit model. STUDY DESIGN: Eighteen does were alloimmunized to incompatible red blood cells. Does were bred twice, once with a homozygous buck of incompatible blood type and once with a homozygous buck of compatible blood type. Fetal blood sampling was undertaken on day 27 of gestation (term 28 to 31 days). Does were delivered on day 30 and the neonatal pups were anesthetized. Direct cardiac samplings were performed for hemoglobin, reticulocyte count, and direct Coombs' test. Hepatic, splenic, and renal wet weights were measured. RESULTS: Twenty-two pregnancies (12 compatible and 10 incompatible) were studied. Neonatal hemoglobin was higher in the compatible litters (11.1 gm/dL [7.7 to 12.6 gm/dL] vs 4.9 gm/dL [2.1 to 9.1 gm/dL], P < .001), whereas no difference could be detected between the respective reticulocyte counts (34.0/100 red blood cells [27.3 to 36.1/100 red blood cells] vs 32.6/100 red blood cells [26.8 to 43.5/100 red blood cells], P = .55). The direct Coombs' assay was negative in 23 pups from 8 compatible litters and false positive (weakly positive result) in 2 pups of a ninth compatible litter. The Coombs' assay was positive in all 22 incompatible pups tested. Hepatosplenomegaly was noted in affected pups but not in controls. CONCLUSIONS: A disease analogous to human hemolytic disease of the newborn can be induced in the rabbit neonate.

AB - OBJECTIVE: Our purpose was to study the neonatal effects of red blood cell alloimmunization in a rabbit model. STUDY DESIGN: Eighteen does were alloimmunized to incompatible red blood cells. Does were bred twice, once with a homozygous buck of incompatible blood type and once with a homozygous buck of compatible blood type. Fetal blood sampling was undertaken on day 27 of gestation (term 28 to 31 days). Does were delivered on day 30 and the neonatal pups were anesthetized. Direct cardiac samplings were performed for hemoglobin, reticulocyte count, and direct Coombs' test. Hepatic, splenic, and renal wet weights were measured. RESULTS: Twenty-two pregnancies (12 compatible and 10 incompatible) were studied. Neonatal hemoglobin was higher in the compatible litters (11.1 gm/dL [7.7 to 12.6 gm/dL] vs 4.9 gm/dL [2.1 to 9.1 gm/dL], P < .001), whereas no difference could be detected between the respective reticulocyte counts (34.0/100 red blood cells [27.3 to 36.1/100 red blood cells] vs 32.6/100 red blood cells [26.8 to 43.5/100 red blood cells], P = .55). The direct Coombs' assay was negative in 23 pups from 8 compatible litters and false positive (weakly positive result) in 2 pups of a ninth compatible litter. The Coombs' assay was positive in all 22 incompatible pups tested. Hepatosplenomegaly was noted in affected pups but not in controls. CONCLUSIONS: A disease analogous to human hemolytic disease of the newborn can be induced in the rabbit neonate.

KW - Animal model

KW - Hemolytic disease of the newborn

KW - New Zealand Red rabbit

KW - New Zealand White rabbit

KW - Rabbit

KW - Red blood cell alloimmunization

KW - Rhesus disease

UR - http://www.scopus.com/inward/record.url?scp=0031757146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031757146&partnerID=8YFLogxK

M3 - Article

C2 - 9822528

AN - SCOPUS:0031757146

VL - 179

SP - 1353

EP - 1358

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 5

ER -