An animal model for studying the pathogenesis of chikungunya virus infection

Sarah A. Ziegler, Liang Lu, Amelia P A Travassos Da Rosa, Shu Yuan Xiao, Robert B. Tesh

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Newborn and 14-day-old mice inoculated subcutaneously with chikungunya virus (CHIKV) developed lethargy, difficulty walking, dragging of hind limbs, and reduced weight gain within 7-10 days after infection (PI). During the initial 6-7 days PI, the animals had viremia; high levels (10 6-108 PFU) of CHIKV were also present in leg muscle. The virus persisted in muscle for several days after viremia disappeared. The major histopathologic changes were in skeletal muscle, which were focal necrosis and inflammation, followed by fibrosis and dystrophic calcification. Some mice also showed dystrophic calcification in the joint cartilage, but there were few deaths, and most of the animals eventually recovered. CHIKV antigen was shown by immunohistochemistry in the muscle for several weeks after infection. Based on the clinical and pathologic similarities with CHIKV infection in humans, young ICR and CD-1 mice offer a useful and realistic model for further study of the pathogenesis and treatment of CHIKV infection.

Original languageEnglish (US)
Pages (from-to)133-139
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume79
Issue number1
StatePublished - Jul 2008

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Chikungunya virus
Animal Models
Viremia
Muscles
Mobility Limitation
Lethargy
Infection
Weight Gain
Cartilage
Leg
Skeletal Muscle
Fibrosis
Necrosis
Extremities
Joints
Immunohistochemistry
Viruses
Inflammation
Antigens
Chikungunya Fever

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Ziegler, S. A., Lu, L., Travassos Da Rosa, A. P. A., Xiao, S. Y., & Tesh, R. B. (2008). An animal model for studying the pathogenesis of chikungunya virus infection. American Journal of Tropical Medicine and Hygiene, 79(1), 133-139.

An animal model for studying the pathogenesis of chikungunya virus infection. / Ziegler, Sarah A.; Lu, Liang; Travassos Da Rosa, Amelia P A; Xiao, Shu Yuan; Tesh, Robert B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 79, No. 1, 07.2008, p. 133-139.

Research output: Contribution to journalArticle

Ziegler, SA, Lu, L, Travassos Da Rosa, APA, Xiao, SY & Tesh, RB 2008, 'An animal model for studying the pathogenesis of chikungunya virus infection', American Journal of Tropical Medicine and Hygiene, vol. 79, no. 1, pp. 133-139.
Ziegler SA, Lu L, Travassos Da Rosa APA, Xiao SY, Tesh RB. An animal model for studying the pathogenesis of chikungunya virus infection. American Journal of Tropical Medicine and Hygiene. 2008 Jul;79(1):133-139.
Ziegler, Sarah A. ; Lu, Liang ; Travassos Da Rosa, Amelia P A ; Xiao, Shu Yuan ; Tesh, Robert B. / An animal model for studying the pathogenesis of chikungunya virus infection. In: American Journal of Tropical Medicine and Hygiene. 2008 ; Vol. 79, No. 1. pp. 133-139.
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