An animal model of neonatal cytomegalovirus infection

Fernando J. Bravo, Nigel Bourne, Mark R. Schleiss, David I. Bernstein

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cytomegalovirus (CMV) is the most common cause of congenital infection in the developed world and can lead to a life-threatening disease. We therefore developed an animal model to evaluate candidate anti-CMV drugs and to further define the pathogenesis of CMV infections. Newborn guinea pigs were infected by intraperitoneal administration of 106pfu of a virulent salivary gland (SG) passaged guinea pig CMV (gpCMV) within 48h of birth. Inoculation of animals produced 50% overall mortality. A lack of weight gain was also a hallmark of infection. By day 14 after inoculation the weight of gpCMV-infected animals was significantly less than controls (152.9±45g versus 254.7±38.5g, P<0.0001). The most consistent isolation and highest titers of virus were found in the liver and spleen early while lung titers were maximal at day 10. A quantitative competitive PCR (qcPCR) assay confirmed the presence of a high CMV viral load in infected organs. Antiviral treatment with cyclic HPMPC (cHPMPC) for 7 days significantly reduced mortality (1/20 versus 14/20, P<0.001) and viral replication but did not improve weight gain. This model should be useful for further evaluations of the pathogenesis of CMV infections and for evaluation of antiviral drugs and vaccines.

Original languageEnglish (US)
Pages (from-to)41-49
Number of pages9
JournalAntiviral Research
Volume60
Issue number1
DOIs
StatePublished - Sep 1 2003

Fingerprint

Cytomegalovirus Infections
Cytomegalovirus
Animal Models
Viral Load
Weight Gain
Antiviral Agents
Roseolovirus
Guinea Pigs
Mortality
Infection
Salivary Glands
Vaccines
Spleen
Parturition
Weights and Measures
Polymerase Chain Reaction
Lung
Liver
Pharmaceutical Preparations

Keywords

  • Animal model
  • cHPMPC
  • Congenital CMV
  • Guinea pig cytomegalovirus
  • Neonatal CMV

ASJC Scopus subject areas

  • Virology
  • Pharmacology

Cite this

An animal model of neonatal cytomegalovirus infection. / Bravo, Fernando J.; Bourne, Nigel; Schleiss, Mark R.; Bernstein, David I.

In: Antiviral Research, Vol. 60, No. 1, 01.09.2003, p. 41-49.

Research output: Contribution to journalArticle

Bravo, Fernando J. ; Bourne, Nigel ; Schleiss, Mark R. ; Bernstein, David I. / An animal model of neonatal cytomegalovirus infection. In: Antiviral Research. 2003 ; Vol. 60, No. 1. pp. 41-49.
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