Abstract
Highly active antiretroviral therapy has decreased the morbidity and mortality of human immunodeficiency virus (HIV) infection, but latently infected cells remain for prolonged periods. CD4+ CD45RO+ T cells are a major latent virus reservoir in HIV-infected persons. Replication-competent, latently HIV-infected T cells can be generated in vitro by infecting peripheral blood mononuclear cells with HIV and then eliminating the HIV-producing cells with an anti-CD25 immunotoxin (IT). The CD25- latently infected cells then can be eliminated with an anti-CD45RO IT. This study determined whether this IT also could kill latently infected CD4 T cells from HIV-infected persons with or without detectable plasma viremia. The results show that ex vivo treatment of cells from HIV-positive persons by anti-CD45RO IT reduces the frequency of both productively and latently infected cells. In contrast, CD4+ CD45A+ naive T cells and a proportion of CD4+ CD45ROlo memory T cells are spared.
Original language | English (US) |
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Pages (from-to) | 306-314 |
Number of pages | 9 |
Journal | Journal of Infectious Diseases |
Volume | 185 |
Issue number | 3 |
DOIs | |
State | Published - Feb 1 2002 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases