Abstract
Highly active antiretroviral therapy has decreased the morbidity and mortality of human immunodeficiency virus (HIV) infection, but latently infected cells remain for prolonged periods. CD4+ CD45RO+ T cells are a major latent virus reservoir in HIV-infected persons. Replication-competent, latently HIV-infected T cells can be generated in vitro by infecting peripheral blood mononuclear cells with HIV and then eliminating the HIV-producing cells with an anti-CD25 immunotoxin (IT). The CD25- latently infected cells then can be eliminated with an anti-CD45RO IT. This study determined whether this IT also could kill latently infected CD4 T cells from HIV-infected persons with or without detectable plasma viremia. The results show that ex vivo treatment of cells from HIV-positive persons by anti-CD45RO IT reduces the frequency of both productively and latently infected cells. In contrast, CD4+ CD45A+ naive T cells and a proportion of CD4+ CD45ROlo memory T cells are spared.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 306-314 |
| Number of pages | 9 |
| Journal | Journal of Infectious Diseases |
| Volume | 185 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 1 2002 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Immunology
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An anti-CD45RO immunotoxin kills latently infected human immunodeficiency virus (HIV) CD4 T cells in the blood of HIV-positive persons. / Saavedra-Lozano, Jesús; McCoig, Cynthia; Xu, Jinbo; Cao, Yanying; Keiser, Philip; Ghetie, Victor; Siliciano, Robert F.; Siliciano, Janet D.; Picker, Louis J.; Ramilo, Octavio; Vitetta, Ellen S.
In: Journal of Infectious Diseases, Vol. 185, No. 3, 01.02.2002, p. 306-314.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An anti-CD45RO immunotoxin kills latently infected human immunodeficiency virus (HIV) CD4 T cells in the blood of HIV-positive persons
AU - Saavedra-Lozano, Jesús
AU - McCoig, Cynthia
AU - Xu, Jinbo
AU - Cao, Yanying
AU - Keiser, Philip
AU - Ghetie, Victor
AU - Siliciano, Robert F.
AU - Siliciano, Janet D.
AU - Picker, Louis J.
AU - Ramilo, Octavio
AU - Vitetta, Ellen S.
PY - 2002/2/1
Y1 - 2002/2/1
N2 - Highly active antiretroviral therapy has decreased the morbidity and mortality of human immunodeficiency virus (HIV) infection, but latently infected cells remain for prolonged periods. CD4+ CD45RO+ T cells are a major latent virus reservoir in HIV-infected persons. Replication-competent, latently HIV-infected T cells can be generated in vitro by infecting peripheral blood mononuclear cells with HIV and then eliminating the HIV-producing cells with an anti-CD25 immunotoxin (IT). The CD25- latently infected cells then can be eliminated with an anti-CD45RO IT. This study determined whether this IT also could kill latently infected CD4 T cells from HIV-infected persons with or without detectable plasma viremia. The results show that ex vivo treatment of cells from HIV-positive persons by anti-CD45RO IT reduces the frequency of both productively and latently infected cells. In contrast, CD4+ CD45A+ naive T cells and a proportion of CD4+ CD45ROlo memory T cells are spared.
AB - Highly active antiretroviral therapy has decreased the morbidity and mortality of human immunodeficiency virus (HIV) infection, but latently infected cells remain for prolonged periods. CD4+ CD45RO+ T cells are a major latent virus reservoir in HIV-infected persons. Replication-competent, latently HIV-infected T cells can be generated in vitro by infecting peripheral blood mononuclear cells with HIV and then eliminating the HIV-producing cells with an anti-CD25 immunotoxin (IT). The CD25- latently infected cells then can be eliminated with an anti-CD45RO IT. This study determined whether this IT also could kill latently infected CD4 T cells from HIV-infected persons with or without detectable plasma viremia. The results show that ex vivo treatment of cells from HIV-positive persons by anti-CD45RO IT reduces the frequency of both productively and latently infected cells. In contrast, CD4+ CD45A+ naive T cells and a proportion of CD4+ CD45ROlo memory T cells are spared.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036467792&partnerID=8YFLogxK
U2 - 10.1086/338565
DO - 10.1086/338565
M3 - Article
VL - 185
SP - 306
EP - 314
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 3
ER -