An anti-CD45RO immunotoxin kills latently infected human immunodeficiency virus (HIV) CD4 T cells in the blood of HIV-positive persons

Jesús Saavedra-Lozano, Cynthia McCoig, Jinbo Xu, Yanying Cao, Philip Keiser, Victor Ghetie, Robert F. Siliciano, Janet D. Siliciano, Louis J. Picker, Octavio Ramilo, Ellen S. Vitetta

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Highly active antiretroviral therapy has decreased the morbidity and mortality of human immunodeficiency virus (HIV) infection, but latently infected cells remain for prolonged periods. CD4+ CD45RO+ T cells are a major latent virus reservoir in HIV-infected persons. Replication-competent, latently HIV-infected T cells can be generated in vitro by infecting peripheral blood mononuclear cells with HIV and then eliminating the HIV-producing cells with an anti-CD25 immunotoxin (IT). The CD25- latently infected cells then can be eliminated with an anti-CD45RO IT. This study determined whether this IT also could kill latently infected CD4 T cells from HIV-infected persons with or without detectable plasma viremia. The results show that ex vivo treatment of cells from HIV-positive persons by anti-CD45RO IT reduces the frequency of both productively and latently infected cells. In contrast, CD4+ CD45A+ naive T cells and a proportion of CD4+ CD45ROlo memory T cells are spared.

Original languageEnglish (US)
Pages (from-to)306-314
Number of pages9
JournalJournal of Infectious Diseases
Volume185
Issue number3
DOIs
StatePublished - Feb 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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