An Arabidopsis thaliana cDNA complementing a hamster apoptosis suppressor mutant

Patrick Gallois, Tomoko Makishima, Valérie Hecht, Barbara Despres, Michele Laudié, Takeharu Nishimoto, Richard Cooke

Research output: Contribution to journalArticle

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Abstract

Programmed cell death or apoptosis is a process in which unwanted cells are eliminated during growth and development. In mammals, several genes have been identified whose products are necessary to prevent entry into the apoptotic process. We have isolated a clone from an Arabidopsis thaliana cDNA library whose predicted translation product shows highly significant similarity to the mammalian defender against apoptotic death 1 (DAD1) protein. Transformation of the mutant hamster tsBN7 cells, which undergo apoptosis at restrictive temperature, demonstrates that the plant protein is as efficient as human DAD1 in rescuing these hamster cells from apoptosis. In contrast to mammals, Southern hybridisation and genomic data indicate that there are probably two genes in Arabidopsis thaliana. Northern blot analysis shows that AtDAD transcripts are present in all tissues examined, although the abundance of the transcripts is reduced in siliques during the maturation and desiccation phase of the seed. This is the first experimental proof that a homologue of an animal gene involved in apoptosis exists in plants and the first demonstration of complementation of a vertebrate mutant by a plant cDNA. Our results suggest that this process of suppression of apoptosis has been conserved in animals and plants.

Original languageEnglish (US)
Pages (from-to)1325-1331
Number of pages7
JournalPlant Journal
Volume11
Issue number6
DOIs
StatePublished - Jun 1997
Externally publishedYes

Fingerprint

Arabidopsis
hamsters
Cricetinae
apoptosis
Arabidopsis thaliana
Complementary DNA
Apoptosis
mutants
Mammals
Genes
Nucleic Acid Hybridization
Desiccation
Plant Proteins
mammals
death
Gene Library
Growth and Development
genes
Northern Blotting
cells

ASJC Scopus subject areas

  • Plant Science

Cite this

Gallois, P., Makishima, T., Hecht, V., Despres, B., Laudié, M., Nishimoto, T., & Cooke, R. (1997). An Arabidopsis thaliana cDNA complementing a hamster apoptosis suppressor mutant. Plant Journal, 11(6), 1325-1331. https://doi.org/10.1046/j.1365-313X.1997.11061325.x

An Arabidopsis thaliana cDNA complementing a hamster apoptosis suppressor mutant. / Gallois, Patrick; Makishima, Tomoko; Hecht, Valérie; Despres, Barbara; Laudié, Michele; Nishimoto, Takeharu; Cooke, Richard.

In: Plant Journal, Vol. 11, No. 6, 06.1997, p. 1325-1331.

Research output: Contribution to journalArticle

Gallois, P, Makishima, T, Hecht, V, Despres, B, Laudié, M, Nishimoto, T & Cooke, R 1997, 'An Arabidopsis thaliana cDNA complementing a hamster apoptosis suppressor mutant', Plant Journal, vol. 11, no. 6, pp. 1325-1331. https://doi.org/10.1046/j.1365-313X.1997.11061325.x
Gallois, Patrick ; Makishima, Tomoko ; Hecht, Valérie ; Despres, Barbara ; Laudié, Michele ; Nishimoto, Takeharu ; Cooke, Richard. / An Arabidopsis thaliana cDNA complementing a hamster apoptosis suppressor mutant. In: Plant Journal. 1997 ; Vol. 11, No. 6. pp. 1325-1331.
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