An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope

Jing Jin, Michael B. Sherman, Daniel Chafets, Nuntana Dinglasan, Kai Lu, Tzong Hae Lee, Lars Anders Carlson, Marcus O. Muench, Graham Simmons

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Abstract

Background: Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. Methodology/Principal findings: To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice. Conclusions/Significance: Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases.

Original languageEnglish (US)
Article numbere0006693
JournalPLoS neglected tropical diseases
Volume12
Issue number7
DOIs
StatePublished - Jul 31 2018

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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    Jin, J., Sherman, M. B., Chafets, D., Dinglasan, N., Lu, K., Lee, T. H., Carlson, L. A., Muench, M. O., & Simmons, G. (2018). An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. PLoS neglected tropical diseases, 12(7), [e0006693]. https://doi.org/10.1371/journal.pntd.0006693