An Endothelial Cell-Surface Form of Annexin II Binds Human Cytomegalovirus

J. F. Wright, A. Kurosky, S. Wasi

    Research output: Contribution to journalArticle

    69 Citations (Scopus)

    Abstract

    Human cytomegalovirus was shown to bind to human umbilical vein endothelial cells in a specific, saturable and calcium-dependent manner (Kd = 7.9 pM (4 °C), 6469 virus binding sites/cell). Affinity adsorption of detergent-prepared lysates of surface-radiolabeled endothelial cells to virions resulted in the identification of cell-derived proteins of approximate Mr 36,000 and 32,000 that bound cytomegalovirus. Protein sequencing of peptides obtained by cyanogen bromide cleavage demonstrated that the 36 kDa protein corresponded to human annexin II, and the 32 kDa protein was likely a degradation product. Purified annexin II was demonstrated to bind directly to virions (Kd = 57 nM, 688 annexin II binding sites/virion). These results provide evidence that an endothelial cell-surface form of annexin II acts as a receptor for cytomegalovirus, and indicate a previously undescribed role for annexin II.

    Original languageEnglish (US)
    Pages (from-to)983-989
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume198
    Issue number3
    DOIs
    StatePublished - Feb 14 1994

    Fingerprint

    Annexin A2
    Endothelial cells
    Cytomegalovirus
    Endothelial Cells
    Virion
    Proteins
    Binding Sites
    Virus Attachment
    Cyanogen Bromide
    Protein Sequence Analysis
    Human Umbilical Vein Endothelial Cells
    Viruses
    Detergents
    Adsorption
    Calcium
    Degradation
    Peptides

    ASJC Scopus subject areas

    • Molecular Biology
    • Biophysics
    • Biochemistry

    Cite this

    An Endothelial Cell-Surface Form of Annexin II Binds Human Cytomegalovirus. / Wright, J. F.; Kurosky, A.; Wasi, S.

    In: Biochemical and Biophysical Research Communications, Vol. 198, No. 3, 14.02.1994, p. 983-989.

    Research output: Contribution to journalArticle

    Wright, J. F. ; Kurosky, A. ; Wasi, S. / An Endothelial Cell-Surface Form of Annexin II Binds Human Cytomegalovirus. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 198, No. 3. pp. 983-989.
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