An evaluation of Congo red fluorescence for the diagnosis of amyloidosis

Cecilia Clement, Luan D. Truong

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Congo red stain apple-green birefringence under polarized light is the most popular method for detecting amyloid; however, it has limitations. The goal of this study was to evaluate if examination of Congo red stain by fluorescent microscopy (FM) significantly enhances the diagnostic yield. Congo red-stained tissue sections were retrospectively and prospectively examined by light microscopy (LM) with and without polarizer and by FM using the Texas red filter and results by each method compared. Congo red-stained amyloid recognized by LM was unequivocally and easily identified by FM in each of 48 cases. In 22 of them, FM either confirmed the presence of a small amount of amyloid or lead to a definitive diagnosis, which was otherwise missed. Eight cases with Congo red-negative by LM were also negative by FM. In 8 cases with a false-positive Congo red stain, FM still detected the signal in 5, but it was absent in 3 cases. In conclusion, Congo red fluorescence improves the diagnostic yield of LM for both positive and negative cases.

Original languageEnglish (US)
Pages (from-to)1766-1772
Number of pages7
JournalHuman Pathology
Volume45
Issue number8
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Congo Red
Amyloidosis
Microscopy
Fluorescence
Light
Amyloid
Coloring Agents
Birefringence

Keywords

  • Amyloid
  • Birefringence
  • Congo red stain
  • Fluorescence
  • Polarized light
  • Texas red filter

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

An evaluation of Congo red fluorescence for the diagnosis of amyloidosis. / Clement, Cecilia; Truong, Luan D.

In: Human Pathology, Vol. 45, No. 8, 2014, p. 1766-1772.

Research output: Contribution to journalArticle

@article{f2f76e4e3f144148aa34d7dae8f6bdb1,
title = "An evaluation of Congo red fluorescence for the diagnosis of amyloidosis",
abstract = "Congo red stain apple-green birefringence under polarized light is the most popular method for detecting amyloid; however, it has limitations. The goal of this study was to evaluate if examination of Congo red stain by fluorescent microscopy (FM) significantly enhances the diagnostic yield. Congo red-stained tissue sections were retrospectively and prospectively examined by light microscopy (LM) with and without polarizer and by FM using the Texas red filter and results by each method compared. Congo red-stained amyloid recognized by LM was unequivocally and easily identified by FM in each of 48 cases. In 22 of them, FM either confirmed the presence of a small amount of amyloid or lead to a definitive diagnosis, which was otherwise missed. Eight cases with Congo red-negative by LM were also negative by FM. In 8 cases with a false-positive Congo red stain, FM still detected the signal in 5, but it was absent in 3 cases. In conclusion, Congo red fluorescence improves the diagnostic yield of LM for both positive and negative cases.",
keywords = "Amyloid, Birefringence, Congo red stain, Fluorescence, Polarized light, Texas red filter",
author = "Cecilia Clement and Truong, {Luan D.}",
year = "2014",
doi = "10.1016/j.humpath.2014.04.016",
language = "English (US)",
volume = "45",
pages = "1766--1772",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "8",

}

TY - JOUR

T1 - An evaluation of Congo red fluorescence for the diagnosis of amyloidosis

AU - Clement, Cecilia

AU - Truong, Luan D.

PY - 2014

Y1 - 2014

N2 - Congo red stain apple-green birefringence under polarized light is the most popular method for detecting amyloid; however, it has limitations. The goal of this study was to evaluate if examination of Congo red stain by fluorescent microscopy (FM) significantly enhances the diagnostic yield. Congo red-stained tissue sections were retrospectively and prospectively examined by light microscopy (LM) with and without polarizer and by FM using the Texas red filter and results by each method compared. Congo red-stained amyloid recognized by LM was unequivocally and easily identified by FM in each of 48 cases. In 22 of them, FM either confirmed the presence of a small amount of amyloid or lead to a definitive diagnosis, which was otherwise missed. Eight cases with Congo red-negative by LM were also negative by FM. In 8 cases with a false-positive Congo red stain, FM still detected the signal in 5, but it was absent in 3 cases. In conclusion, Congo red fluorescence improves the diagnostic yield of LM for both positive and negative cases.

AB - Congo red stain apple-green birefringence under polarized light is the most popular method for detecting amyloid; however, it has limitations. The goal of this study was to evaluate if examination of Congo red stain by fluorescent microscopy (FM) significantly enhances the diagnostic yield. Congo red-stained tissue sections were retrospectively and prospectively examined by light microscopy (LM) with and without polarizer and by FM using the Texas red filter and results by each method compared. Congo red-stained amyloid recognized by LM was unequivocally and easily identified by FM in each of 48 cases. In 22 of them, FM either confirmed the presence of a small amount of amyloid or lead to a definitive diagnosis, which was otherwise missed. Eight cases with Congo red-negative by LM were also negative by FM. In 8 cases with a false-positive Congo red stain, FM still detected the signal in 5, but it was absent in 3 cases. In conclusion, Congo red fluorescence improves the diagnostic yield of LM for both positive and negative cases.

KW - Amyloid

KW - Birefringence

KW - Congo red stain

KW - Fluorescence

KW - Polarized light

KW - Texas red filter

UR - http://www.scopus.com/inward/record.url?scp=84904635401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904635401&partnerID=8YFLogxK

U2 - 10.1016/j.humpath.2014.04.016

DO - 10.1016/j.humpath.2014.04.016

M3 - Article

VL - 45

SP - 1766

EP - 1772

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 8

ER -