An experimental model combining microdialysis with electrophysiology, histology, and neurochemistry for studying excitotoxicity in spinal cord injury - Effect of NMDA and Kainate

Danxia Liu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We used an experimental model that we previously developed to characterize the damage caused by the agonists of glutamate receptors, N-methyl-d-aspartate (NMDA) and kainate, in the spinal cord in vivo, thereby testing further the utility of this model. Microdialysis was used to, administer the toxins and to sample the release of other substances in response to these agents. The blockage of electrical conduction was monitored by recording the amplitudes of evoked potentials during administration of the damaging substances, and damage was assessed, by postmortem histological examination. The released amino acids in microdialysates were measured by HPLC. Administration of 5 m M NMDA+5m M kainate into the gray matter blocked most postsynaptic responses and caused the release of amino acids. Administration of 10 m M NMDA and 10 m M kainate significantly destroyed cell bodies near the fiber. The advantage of this model is that histological, neurochemical, and electrophysiological parameters were obtained in the same experiment.

Original languageEnglish (US)
Pages (from-to)77-92
Number of pages16
JournalMolecular and Chemical Neuropathology
Volume23
Issue number2-3
DOIs
StatePublished - Oct 1994

Fingerprint

Neurochemistry
Kainic Acid
Electrophysiology
Microdialysis
Spinal Cord Injuries
Aspartic Acid
Histology
Theoretical Models
Amino Acids
Glutamate Receptors
Evoked Potentials
Autopsy
Spinal Cord
High Pressure Liquid Chromatography

Keywords

  • Glutamate
  • kainate
  • microdialysis
  • N-methyl-d-aspartate
  • secondary damage
  • spinal cord injury

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Molecular Biology

Cite this

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