An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat

Sun Ho Kim, Jin Chung

Research output: Contribution to journalArticle

2417 Citations (Scopus)

Abstract

We attempted to develop an experimental animal model for peripheral neuropathic pain. Under sodium pentobarbital anesthesia, both the L5 and L6 spinal nerves (group 1) or the L5 spinal nerve alone (group 2) of one side of the rat were tightly ligated. For comparison, a parallel study was conducted with another group of rats (group 3) which received a partial tight sciatic nerve ligation, a paradigm developed previously as a neuropathy model. Withdrawal latencies to application of radiant heat to the foot were tested for the next 16 weeks in all 3 groups. Sensitivity of the hind paw to mechanical stimulation was tested with von Frey filaments. The general behavior of each rat was noted during the entire test period. Results suggested that the surgical procedure in all 3 groups produced a long-lasting hyperalgesia to noxious heat (at least 5 weeks) and mechanical allodynia (at least 10 weeks) of the affected foot. In addition, there were behavioral signs of the presence of spontaneous pain in the affected foot. Therefore, we believe we have developed an experimental animal model for peripheral neuropathy using tight ligations of spinal nerves. The model manifests the symptoms of human patients with causalgia and is compatible with a previously developed neuropathy model. The present model has two unique features. First, the surgical procedure is stereotyped. Second, the levels of injured and intact spinal segments are completely separated, allowing independent experimental manipulations of the injured and intact spinal segments in future experiments to answer questions regarding mechanisms underlying causalgia.

Original languageEnglish (US)
Pages (from-to)355-363
Number of pages9
JournalPain
Volume50
Issue number3
DOIs
StatePublished - 1992

Fingerprint

Spinal Nerves
Peripheral Nervous System Diseases
Ligation
Foot
Theoretical Models
Hyperalgesia
Animal Models
Hot Temperature
Neuralgia
Sciatic Nerve
Pentobarbital
Anesthesia
Pain

Keywords

  • Allodynia
  • Causalgia
  • Hyperalgesia
  • Peripheral nerve injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Psychiatry and Mental health
  • Neuroscience(all)
  • Neurology
  • Pharmacology
  • Clinical Psychology

Cite this

An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. / Ho Kim, Sun; Chung, Jin.

In: Pain, Vol. 50, No. 3, 1992, p. 355-363.

Research output: Contribution to journalArticle

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