An exponential gradient maker for use with minigel polyacrylamide electrophoresis systems

Thomas J. Smith; J. Ellis Bell

doi:10.1016/0003-2697(86)90121-1

An exponential gradient maker for use with minigel polyacrylamide electrophoresis systems

Thomas J. Smith, J. Ellis Bell

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Minigel electrophoresis has reduced the required sample size and cost of running polyacrylamide gels without loss of resolution. The many problems incurred in the minigel system when pouring multiple gradient gels are eliminated by pouring individal gels with the described economical, exponential gradient maker, which can accurately deal with the small gel volumes. The advantages of exponential gradients are discussed.

Original language	English (US)
Pages (from-to)	74-77
Number of pages	4
Journal	Analytical Biochemistry
Volume	152
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(86)90121-1
State	Published - Jan 1986
Externally published	Yes

Keywords

electrophoresis
gel electrophoresis
proteins

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0003-2697(86)90121-1

Cite this

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title = "An exponential gradient maker for use with minigel polyacrylamide electrophoresis systems",

abstract = "Minigel electrophoresis has reduced the required sample size and cost of running polyacrylamide gels without loss of resolution. The many problems incurred in the minigel system when pouring multiple gradient gels are eliminated by pouring individal gels with the described economical, exponential gradient maker, which can accurately deal with the small gel volumes. The advantages of exponential gradients are discussed.",

keywords = "electrophoresis, gel electrophoresis, proteins",

author = "Smith, {Thomas J.} and Bell, {J. Ellis}",

note = "Funding Information: by NIH Grant GM 30195.",

year = "1986",

month = jan,

doi = "10.1016/0003-2697(86)90121-1",

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AU - Bell, J. Ellis

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PY - 1986/1

Y1 - 1986/1

N2 - Minigel electrophoresis has reduced the required sample size and cost of running polyacrylamide gels without loss of resolution. The many problems incurred in the minigel system when pouring multiple gradient gels are eliminated by pouring individal gels with the described economical, exponential gradient maker, which can accurately deal with the small gel volumes. The advantages of exponential gradients are discussed.

AB - Minigel electrophoresis has reduced the required sample size and cost of running polyacrylamide gels without loss of resolution. The many problems incurred in the minigel system when pouring multiple gradient gels are eliminated by pouring individal gels with the described economical, exponential gradient maker, which can accurately deal with the small gel volumes. The advantages of exponential gradients are discussed.

KW - electrophoresis

KW - gel electrophoresis

KW - proteins

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ER -

An exponential gradient maker for use with minigel polyacrylamide electrophoresis systems

Abstract

Keywords

ASJC Scopus subject areas

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