An exponential gradient maker for use with minigel polyacrylamide electrophoresis systems

Thomas J. Smith, J. Ellis Bell

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Minigel electrophoresis has reduced the required sample size and cost of running polyacrylamide gels without loss of resolution. The many problems incurred in the minigel system when pouring multiple gradient gels are eliminated by pouring individal gels with the described economical, exponential gradient maker, which can accurately deal with the small gel volumes. The advantages of exponential gradients are discussed.

Original languageEnglish
Pages (from-to)74-77
Number of pages4
JournalAnalytical Biochemistry
Volume152
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

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Keywords

  • electrophoresis
  • gel electrophoresis
  • proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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