An immunological fingerprint differentiates muscular lymphatics from arteries and veins

Eric A. Bridenbaugh, Wei Wang, Maya Srimushnam, Walter E. Cromer, Scott D. Zawieja, Susan E. Schmidt, Daniel Jupiter, Hung Chung Huang, Vincent Van Buren, David C. Zawieja

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The principal function of the lymphatic system is to transport lymph from the interstitium to the nodes and then from the nodes to the blood. In doing so lymphatics play important roles in fluid homeostasis, macromolecular/antigen transport and immune cell trafficking. To better understand the genes that contribute to their unique physiology, we compared the transcriptional profile of muscular lymphatics (prenodal mesenteric microlymphatics and large, postnodal thoracic duct) to axillary and mesenteric arteries and veins isolated from rats. Clustering of the differentially expressed genes demonstrated that the lymph versus blood vessel differences were more profound than between blood vessels, particularly the microvessels. Gene ontology functional category analysis indicated that microlymphatics were enriched in antigen processing/presentation, IgE receptor signaling, catabolic processes, translation and ribosome; while they were diminished in oxygen transport, regulation of cell proliferation, glycolysis and inhibition of adenylate cyclase activity by G-proteins. We evaluated the differentially expressed microarray genes/products by qPCR and/or immunofluorescence. Immunofluorescence documented that multiple MHC class II antigen presentation proteins were highly expressed by an antigen-presenting cell (APC) type found resident within the lymphatic wall. These APCs also expressed CD86, a co-stimulatory protein necessary for T-cell activation. We evaluated the distribution and phenotype of APCs within the pre and postnodal lymphatic network. This study documents a novel population of APCs resident within the walls of muscular, prenodal lymphatics that indicates novel roles in antigen sampling and immune responses. In conclusion, these prenodal lymphatics exhibit a unique profile that distinguishes them from blood vessels and highlights the role of the lymphatic system as an immunovascular system linking the parenchymal interstitium, lymph nodes and the blood.

Original languageEnglish (US)
Pages (from-to)155-171
Number of pages17
JournalLymphatic Research and Biology
Volume11
Issue number3
DOIs
StatePublished - Sep 1 2013
Externally publishedYes

Fingerprint

Antigen Presentation
Dermatoglyphics
Blood Vessels
Veins
Lymphatic System
Arteries
Histocompatibility Antigens Class II
Lymph
Fluorescent Antibody Technique
Axillary Vein
Axillary Artery
Genes
IgE Receptors
Mesenteric Veins
Thoracic Duct
Gene Ontology
Mesenteric Arteries
Glycolysis
Antigen-Presenting Cells
Microvessels

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Bridenbaugh, E. A., Wang, W., Srimushnam, M., Cromer, W. E., Zawieja, S. D., Schmidt, S. E., ... Zawieja, D. C. (2013). An immunological fingerprint differentiates muscular lymphatics from arteries and veins. Lymphatic Research and Biology, 11(3), 155-171. https://doi.org/10.1089/lrb.2013.0023

An immunological fingerprint differentiates muscular lymphatics from arteries and veins. / Bridenbaugh, Eric A.; Wang, Wei; Srimushnam, Maya; Cromer, Walter E.; Zawieja, Scott D.; Schmidt, Susan E.; Jupiter, Daniel; Huang, Hung Chung; Van Buren, Vincent; Zawieja, David C.

In: Lymphatic Research and Biology, Vol. 11, No. 3, 01.09.2013, p. 155-171.

Research output: Contribution to journalArticle

Bridenbaugh, EA, Wang, W, Srimushnam, M, Cromer, WE, Zawieja, SD, Schmidt, SE, Jupiter, D, Huang, HC, Van Buren, V & Zawieja, DC 2013, 'An immunological fingerprint differentiates muscular lymphatics from arteries and veins', Lymphatic Research and Biology, vol. 11, no. 3, pp. 155-171. https://doi.org/10.1089/lrb.2013.0023
Bridenbaugh EA, Wang W, Srimushnam M, Cromer WE, Zawieja SD, Schmidt SE et al. An immunological fingerprint differentiates muscular lymphatics from arteries and veins. Lymphatic Research and Biology. 2013 Sep 1;11(3):155-171. https://doi.org/10.1089/lrb.2013.0023
Bridenbaugh, Eric A. ; Wang, Wei ; Srimushnam, Maya ; Cromer, Walter E. ; Zawieja, Scott D. ; Schmidt, Susan E. ; Jupiter, Daniel ; Huang, Hung Chung ; Van Buren, Vincent ; Zawieja, David C. / An immunological fingerprint differentiates muscular lymphatics from arteries and veins. In: Lymphatic Research and Biology. 2013 ; Vol. 11, No. 3. pp. 155-171.
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