An integrated system for identifying the hidden assassins in traditional medicines containing aristolochic acids

Lan Wu, Wei Sun, Bo Wang, Haiyu Zhao, Yaoli Li, Shaoqing Cai, Li Xiang, Yingjie Zhu, Hui Yao, Jingyuan Song, Yung Chi Cheng, Shilin Chen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Traditional herbal medicines adulterated and contaminated with plant materials from the Aristolochiaceae family, which contain aristolochic acids (AAs), cause aristolochic acid nephropathy. Approximately 256 traditional Chinese patent medicines, containing Aristolochiaceous materials, are still being sold in Chinese markets today. In order to protect consumers from health risks due to AAs, the hidden assassins, efficient methods to differentiate Aristolochiaceous herbs from their putative substitutes need to be established. In this study, 158 Aristolochiaceous samples representing 46 species and four genera as well as 131 non-Aristolochiaceous samples representing 33 species, 20 genera and 12 families were analyzed using DNA barcodes based on the ITS2 and psbA-trnH sequences. Aristolochiaceous materials and their non-Aristolochiaceous substitutes were successfully identified using BLAST1, the nearest distance method and the neighbor-joining (NJ) tree. In addition, based on sequence information of ITS2, we developed a Real-Time PCR assay which successfully identified herbal material from the Aristolochiaceae family. Using Ultra High Performance Liquid Chromatography-Mass Spectrometer (UHPLC-HR-MS), we demonstrated that most representatives from the Aristolochiaceae family contain toxic AAs. Therefore, integrated DNA barcodes, Real-Time PCR assays using TaqMan probes and UHPLC-HR-MS system provides an efficient and reliable authentication system to protect consumers from health risks due to the hidden assassins (AAs).

Original languageEnglish (US)
Article number11318
JournalScientific Reports
Volume5
DOIs
StatePublished - Aug 13 2015
Externally publishedYes

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Aristolochiaceae
Aristolochic Acids
Traditional Medicine
Real-Time Polymerase Chain Reaction
High Pressure Liquid Chromatography
Nonprescription Drugs
Herbal Medicine
Poisons
DNA
Health

ASJC Scopus subject areas

  • General

Cite this

An integrated system for identifying the hidden assassins in traditional medicines containing aristolochic acids. / Wu, Lan; Sun, Wei; Wang, Bo; Zhao, Haiyu; Li, Yaoli; Cai, Shaoqing; Xiang, Li; Zhu, Yingjie; Yao, Hui; Song, Jingyuan; Cheng, Yung Chi; Chen, Shilin.

In: Scientific Reports, Vol. 5, 11318, 13.08.2015.

Research output: Contribution to journalArticle

Wu, L, Sun, W, Wang, B, Zhao, H, Li, Y, Cai, S, Xiang, L, Zhu, Y, Yao, H, Song, J, Cheng, YC & Chen, S 2015, 'An integrated system for identifying the hidden assassins in traditional medicines containing aristolochic acids', Scientific Reports, vol. 5, 11318. https://doi.org/10.1038/srep11318
Wu, Lan ; Sun, Wei ; Wang, Bo ; Zhao, Haiyu ; Li, Yaoli ; Cai, Shaoqing ; Xiang, Li ; Zhu, Yingjie ; Yao, Hui ; Song, Jingyuan ; Cheng, Yung Chi ; Chen, Shilin. / An integrated system for identifying the hidden assassins in traditional medicines containing aristolochic acids. In: Scientific Reports. 2015 ; Vol. 5.
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abstract = "Traditional herbal medicines adulterated and contaminated with plant materials from the Aristolochiaceae family, which contain aristolochic acids (AAs), cause aristolochic acid nephropathy. Approximately 256 traditional Chinese patent medicines, containing Aristolochiaceous materials, are still being sold in Chinese markets today. In order to protect consumers from health risks due to AAs, the hidden assassins, efficient methods to differentiate Aristolochiaceous herbs from their putative substitutes need to be established. In this study, 158 Aristolochiaceous samples representing 46 species and four genera as well as 131 non-Aristolochiaceous samples representing 33 species, 20 genera and 12 families were analyzed using DNA barcodes based on the ITS2 and psbA-trnH sequences. Aristolochiaceous materials and their non-Aristolochiaceous substitutes were successfully identified using BLAST1, the nearest distance method and the neighbor-joining (NJ) tree. In addition, based on sequence information of ITS2, we developed a Real-Time PCR assay which successfully identified herbal material from the Aristolochiaceae family. Using Ultra High Performance Liquid Chromatography-Mass Spectrometer (UHPLC-HR-MS), we demonstrated that most representatives from the Aristolochiaceae family contain toxic AAs. Therefore, integrated DNA barcodes, Real-Time PCR assays using TaqMan probes and UHPLC-HR-MS system provides an efficient and reliable authentication system to protect consumers from health risks due to the hidden assassins (AAs).",
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