An Intranasal Exposure Model of Lethal Nipah Virus Infection in African Green Monkeys

Joan B. Geisbert; Viktoriya Borisevich; Abhishek N. Prasad; Krystle N. Agans; Stephanie L. Foster; Daniel J. Deer; Robert W. Cross; Chad E. Mire; Thomas W. Geisbert; Karla A. Fenton

doi:10.1093/infdis/jiz391

An Intranasal Exposure Model of Lethal Nipah Virus Infection in African Green Monkeys

Joan B. Geisbert, Viktoriya Borisevich, Abhishek N. Prasad, Krystle N. Agans, Stephanie L. Foster, Daniel J. Deer, Robert W. Cross, Chad E. Mire, Thomas W. Geisbert, Karla A. Fenton

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Due to the difficulty in conducting clinical trials for vaccines and treatments against Nipah virus (NiV), licensure will likely require animal models, most importantly non-human primates (NHPs). The NHP models of infection have primarily relied on intratracheal instillation or small particle aerosolization of NiV. However, neither of these routes adequately models natural mucosal exposure to NiV. To develop a more natural NHP model, we challenged African green monkeys with the Bangladesh strain of NiV by the intranasal route using the laryngeal mask airway (LMA) mucosal atomization device (MAD). LMA MAD exposure resulted in uniformly lethal disease that accurately reflected the human condition.

Original language	English (US)
Pages (from-to)	S414-S418
Journal	Journal of Infectious Diseases
Volume	221
DOIs	https://doi.org/10.1093/infdis/jiz391
State	Published - May 11 2020

Keywords

Nipah virus
animal model
henipavirus
pathogenesis
primate

ASJC Scopus subject areas

General Medicine

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10.1093/infdis/jiz391

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title = "An Intranasal Exposure Model of Lethal Nipah Virus Infection in African Green Monkeys",

abstract = "Due to the difficulty in conducting clinical trials for vaccines and treatments against Nipah virus (NiV), licensure will likely require animal models, most importantly non-human primates (NHPs). The NHP models of infection have primarily relied on intratracheal instillation or small particle aerosolization of NiV. However, neither of these routes adequately models natural mucosal exposure to NiV. To develop a more natural NHP model, we challenged African green monkeys with the Bangladesh strain of NiV by the intranasal route using the laryngeal mask airway (LMA) mucosal atomization device (MAD). LMA MAD exposure resulted in uniformly lethal disease that accurately reflected the human condition.",

keywords = "Nipah virus, animal model, henipavirus, pathogenesis, primate",

author = "Geisbert, {Joan B.} and Viktoriya Borisevich and Prasad, {Abhishek N.} and Agans, {Krystle N.} and Foster, {Stephanie L.} and Deer, {Daniel J.} and Cross, {Robert W.} and Mire, {Chad E.} and Geisbert, {Thomas W.} and Fenton, {Karla A.}",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].",

year = "2020",

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doi = "10.1093/infdis/jiz391",

language = "English (US)",

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AU - Geisbert, Joan B.

AU - Borisevich, Viktoriya

AU - Prasad, Abhishek N.

AU - Agans, Krystle N.

AU - Foster, Stephanie L.

AU - Deer, Daniel J.

AU - Cross, Robert W.

AU - Mire, Chad E.

AU - Geisbert, Thomas W.

AU - Fenton, Karla A.

N1 - Publisher Copyright: © 2019 The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

PY - 2020/5/11

Y1 - 2020/5/11

N2 - Due to the difficulty in conducting clinical trials for vaccines and treatments against Nipah virus (NiV), licensure will likely require animal models, most importantly non-human primates (NHPs). The NHP models of infection have primarily relied on intratracheal instillation or small particle aerosolization of NiV. However, neither of these routes adequately models natural mucosal exposure to NiV. To develop a more natural NHP model, we challenged African green monkeys with the Bangladesh strain of NiV by the intranasal route using the laryngeal mask airway (LMA) mucosal atomization device (MAD). LMA MAD exposure resulted in uniformly lethal disease that accurately reflected the human condition.

AB - Due to the difficulty in conducting clinical trials for vaccines and treatments against Nipah virus (NiV), licensure will likely require animal models, most importantly non-human primates (NHPs). The NHP models of infection have primarily relied on intratracheal instillation or small particle aerosolization of NiV. However, neither of these routes adequately models natural mucosal exposure to NiV. To develop a more natural NHP model, we challenged African green monkeys with the Bangladesh strain of NiV by the intranasal route using the laryngeal mask airway (LMA) mucosal atomization device (MAD). LMA MAD exposure resulted in uniformly lethal disease that accurately reflected the human condition.

KW - Nipah virus

KW - animal model

KW - henipavirus

KW - pathogenesis

KW - primate

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An Intranasal Exposure Model of Lethal Nipah Virus Infection in African Green Monkeys

Abstract

Keywords

ASJC Scopus subject areas

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