An invasive Mimosa in India does not adopt the symbionts of its native relatives

Hukam Singh Gehlot, Nisha Tak, Muskan Kaushik, Shubhajit Mitra, Wen Ming Chen, Nicole Poweleit, Dheeren Panwar, Neetu Poonar, Rashmita Parihar, Alkesh Tak, Indu Singh Sankhla, Archana Ojha, Satyawada Rama Rao, Marcelo F. Simon, Fabio Bueno Dos Reis Junior, Natalia Perigolo, Anil K. Tripathi, Janet I. Sprent, J. Peter W Young, Euan K. JamesPrasad Gyaneshwar

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.

Original languageEnglish (US)
Pages (from-to)179-196
Number of pages18
JournalAnnals of Botany
Volume112
Issue number1
DOIs
StatePublished - Jul 2013
Externally publishedYes

Fingerprint

Mimosa
symbionts
India
Ensifer
Burkholderia
Rhizobium
Cupriavidus
ribosomal RNA
microsymbionts
beta-Proteobacteria
nodulation
invasive species
light microscopy
rhizosphere
electron microscopy
indigenous species
legumes
nucleotide sequences
Brazil
phylogeny

Keywords

  • arid regions
  • bacterial symbionts
  • Betaproteobacteria
  • Burkholderia
  • Cupriavidus
  • Ensifer
  • Mimosa hamata
  • Mimosa himalayana
  • Mimosa pudica
  • nitrogen fixation
  • nodulation
  • rhizobia
  • Thar Desert

ASJC Scopus subject areas

  • Plant Science

Cite this

Gehlot, H. S., Tak, N., Kaushik, M., Mitra, S., Chen, W. M., Poweleit, N., ... Gyaneshwar, P. (2013). An invasive Mimosa in India does not adopt the symbionts of its native relatives. Annals of Botany, 112(1), 179-196. https://doi.org/10.1093/aob/mct112

An invasive Mimosa in India does not adopt the symbionts of its native relatives. / Gehlot, Hukam Singh; Tak, Nisha; Kaushik, Muskan; Mitra, Shubhajit; Chen, Wen Ming; Poweleit, Nicole; Panwar, Dheeren; Poonar, Neetu; Parihar, Rashmita; Tak, Alkesh; Sankhla, Indu Singh; Ojha, Archana; Rao, Satyawada Rama; Simon, Marcelo F.; Reis Junior, Fabio Bueno Dos; Perigolo, Natalia; Tripathi, Anil K.; Sprent, Janet I.; Young, J. Peter W; James, Euan K.; Gyaneshwar, Prasad.

In: Annals of Botany, Vol. 112, No. 1, 07.2013, p. 179-196.

Research output: Contribution to journalArticle

Gehlot, HS, Tak, N, Kaushik, M, Mitra, S, Chen, WM, Poweleit, N, Panwar, D, Poonar, N, Parihar, R, Tak, A, Sankhla, IS, Ojha, A, Rao, SR, Simon, MF, Reis Junior, FBD, Perigolo, N, Tripathi, AK, Sprent, JI, Young, JPW, James, EK & Gyaneshwar, P 2013, 'An invasive Mimosa in India does not adopt the symbionts of its native relatives', Annals of Botany, vol. 112, no. 1, pp. 179-196. https://doi.org/10.1093/aob/mct112
Gehlot HS, Tak N, Kaushik M, Mitra S, Chen WM, Poweleit N et al. An invasive Mimosa in India does not adopt the symbionts of its native relatives. Annals of Botany. 2013 Jul;112(1):179-196. https://doi.org/10.1093/aob/mct112
Gehlot, Hukam Singh ; Tak, Nisha ; Kaushik, Muskan ; Mitra, Shubhajit ; Chen, Wen Ming ; Poweleit, Nicole ; Panwar, Dheeren ; Poonar, Neetu ; Parihar, Rashmita ; Tak, Alkesh ; Sankhla, Indu Singh ; Ojha, Archana ; Rao, Satyawada Rama ; Simon, Marcelo F. ; Reis Junior, Fabio Bueno Dos ; Perigolo, Natalia ; Tripathi, Anil K. ; Sprent, Janet I. ; Young, J. Peter W ; James, Euan K. ; Gyaneshwar, Prasad. / An invasive Mimosa in India does not adopt the symbionts of its native relatives. In: Annals of Botany. 2013 ; Vol. 112, No. 1. pp. 179-196.
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title = "An invasive Mimosa in India does not adopt the symbionts of its native relatives",
abstract = "Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.",
keywords = "arid regions, bacterial symbionts, Betaproteobacteria, Burkholderia, Cupriavidus, Ensifer, Mimosa hamata, Mimosa himalayana, Mimosa pudica, nitrogen fixation, nodulation, rhizobia, Thar Desert",
author = "Gehlot, {Hukam Singh} and Nisha Tak and Muskan Kaushik and Shubhajit Mitra and Chen, {Wen Ming} and Nicole Poweleit and Dheeren Panwar and Neetu Poonar and Rashmita Parihar and Alkesh Tak and Sankhla, {Indu Singh} and Archana Ojha and Rao, {Satyawada Rama} and Simon, {Marcelo F.} and {Reis Junior}, {Fabio Bueno Dos} and Natalia Perigolo and Tripathi, {Anil K.} and Sprent, {Janet I.} and Young, {J. Peter W} and James, {Euan K.} and Prasad Gyaneshwar",
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T1 - An invasive Mimosa in India does not adopt the symbionts of its native relatives

AU - Gehlot, Hukam Singh

AU - Tak, Nisha

AU - Kaushik, Muskan

AU - Mitra, Shubhajit

AU - Chen, Wen Ming

AU - Poweleit, Nicole

AU - Panwar, Dheeren

AU - Poonar, Neetu

AU - Parihar, Rashmita

AU - Tak, Alkesh

AU - Sankhla, Indu Singh

AU - Ojha, Archana

AU - Rao, Satyawada Rama

AU - Simon, Marcelo F.

AU - Reis Junior, Fabio Bueno Dos

AU - Perigolo, Natalia

AU - Tripathi, Anil K.

AU - Sprent, Janet I.

AU - Young, J. Peter W

AU - James, Euan K.

AU - Gyaneshwar, Prasad

PY - 2013/7

Y1 - 2013/7

N2 - Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.

AB - Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.

KW - arid regions

KW - bacterial symbionts

KW - Betaproteobacteria

KW - Burkholderia

KW - Cupriavidus

KW - Ensifer

KW - Mimosa hamata

KW - Mimosa himalayana

KW - Mimosa pudica

KW - nitrogen fixation

KW - nodulation

KW - rhizobia

KW - Thar Desert

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