Abstract
Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 179-196 |
| Number of pages | 18 |
| Journal | Annals of Botany |
| Volume | 112 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 2013 |
| Externally published | Yes |
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Keywords
- arid regions
- bacterial symbionts
- Betaproteobacteria
- Burkholderia
- Cupriavidus
- Ensifer
- Mimosa hamata
- Mimosa himalayana
- Mimosa pudica
- nitrogen fixation
- nodulation
- rhizobia
- Thar Desert
ASJC Scopus subject areas
- Plant Science
Cite this
An invasive Mimosa in India does not adopt the symbionts of its native relatives. / Gehlot, Hukam Singh; Tak, Nisha; Kaushik, Muskan; Mitra, Shubhajit; Chen, Wen Ming; Poweleit, Nicole; Panwar, Dheeren; Poonar, Neetu; Parihar, Rashmita; Tak, Alkesh; Sankhla, Indu Singh; Ojha, Archana; Rao, Satyawada Rama; Simon, Marcelo F.; Reis Junior, Fabio Bueno Dos; Perigolo, Natalia; Tripathi, Anil K.; Sprent, Janet I.; Young, J. Peter W; James, Euan K.; Gyaneshwar, Prasad.
In: Annals of Botany, Vol. 112, No. 1, 07.2013, p. 179-196.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An invasive Mimosa in India does not adopt the symbionts of its native relatives
AU - Gehlot, Hukam Singh
AU - Tak, Nisha
AU - Kaushik, Muskan
AU - Mitra, Shubhajit
AU - Chen, Wen Ming
AU - Poweleit, Nicole
AU - Panwar, Dheeren
AU - Poonar, Neetu
AU - Parihar, Rashmita
AU - Tak, Alkesh
AU - Sankhla, Indu Singh
AU - Ojha, Archana
AU - Rao, Satyawada Rama
AU - Simon, Marcelo F.
AU - Reis Junior, Fabio Bueno Dos
AU - Perigolo, Natalia
AU - Tripathi, Anil K.
AU - Sprent, Janet I.
AU - Young, J. Peter W
AU - James, Euan K.
AU - Gyaneshwar, Prasad
PY - 2013/7
Y1 - 2013/7
N2 - Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.
AB - Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.
KW - arid regions
KW - bacterial symbionts
KW - Betaproteobacteria
KW - Burkholderia
KW - Cupriavidus
KW - Ensifer
KW - Mimosa hamata
KW - Mimosa himalayana
KW - Mimosa pudica
KW - nitrogen fixation
KW - nodulation
KW - rhizobia
KW - Thar Desert
UR - http://www.scopus.com/inward/record.url?scp=84880199238&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880199238&partnerID=8YFLogxK
U2 - 10.1093/aob/mct112
DO - 10.1093/aob/mct112
M3 - Article
C2 - 23712450
AN - SCOPUS:84880199238
VL - 112
SP - 179
EP - 196
JO - Annals of Botany
JF - Annals of Botany
SN - 0305-7364
IS - 1
ER -