An optofluidic nanoplasmonic biosensor for direct detection of live viruses from biological media

Ahmet A. Yanik, Min Huang, Osami Kamohara, Alp Artar, Thomas Geisbert, John H. Connor, Hatice Altug

Research output: Contribution to journalArticle

264 Citations (Scopus)

Abstract

Fast and sensitive virus detection techniques, which can be rapidly deployed at multiple sites, are essential to prevent and control future epidemics and bioterrorism threats. In this Letter, we demonstrate a label-free optofluidic nanoplasmonic sensor that can directly detect intact viruses from biological media at clinically relevant concentrations with little to no sample preparation. Our sensing platform is based on an extraordinary light transmission effect in plasmonic nanoholes and utilizes group-specific antibodies for highly divergent strains of rapidly evolving viruses. So far, the questions remain for the possible limitations of this technique for virus detection, as the penetration depths of the surface plasmon polaritons are comparable to the dimensions of the pathogens. Here, we demonstrate detection and recognition of small enveloped RNA viruses (vesicular stomatitis virus and pseudotyped Ebola) as well as large enveloped DNA viruses (vaccinia virus) within a dynamic range spanning 3 orders of magnitude. Our platform, by enabling high signal to noise measurements without any mechanical or optical isolation, opens up opportunities for detection of a broad range of pathogens in typical biology laboratory settings.

Original languageEnglish (US)
Pages (from-to)4962-4969
Number of pages8
JournalNano Letters
Volume10
Issue number12
DOIs
StatePublished - Dec 8 2010
Externally publishedYes

Fingerprint

viruses
Viruses
bioinstrumentation
Biosensors
pathogens
Pathogens
platforms
Bioterrorism
light transmission
noise measurement
Light transmission
RNA
antibodies
biology
Antibodies
polaritons
dynamic range
Labels
isolation
DNA

Keywords

  • Biosensing
  • ebola
  • plasmonics
  • vaccinia
  • vesicular stomatitis virus
  • virus detection

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Yanik, A. A., Huang, M., Kamohara, O., Artar, A., Geisbert, T., Connor, J. H., & Altug, H. (2010). An optofluidic nanoplasmonic biosensor for direct detection of live viruses from biological media. Nano Letters, 10(12), 4962-4969. https://doi.org/10.1021/nl103025u

An optofluidic nanoplasmonic biosensor for direct detection of live viruses from biological media. / Yanik, Ahmet A.; Huang, Min; Kamohara, Osami; Artar, Alp; Geisbert, Thomas; Connor, John H.; Altug, Hatice.

In: Nano Letters, Vol. 10, No. 12, 08.12.2010, p. 4962-4969.

Research output: Contribution to journalArticle

Yanik, AA, Huang, M, Kamohara, O, Artar, A, Geisbert, T, Connor, JH & Altug, H 2010, 'An optofluidic nanoplasmonic biosensor for direct detection of live viruses from biological media', Nano Letters, vol. 10, no. 12, pp. 4962-4969. https://doi.org/10.1021/nl103025u
Yanik, Ahmet A. ; Huang, Min ; Kamohara, Osami ; Artar, Alp ; Geisbert, Thomas ; Connor, John H. ; Altug, Hatice. / An optofluidic nanoplasmonic biosensor for direct detection of live viruses from biological media. In: Nano Letters. 2010 ; Vol. 10, No. 12. pp. 4962-4969.
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