An ultrastructural study of the interaction of human eosinophils with respiratory syncytial virus

Jan L L Kimpen, Roberto Garofalo, Robert C. Welliver, Keiji Fujihara, Peary L. Ogra

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

It was shown previously that eosinophils are activated in vivo and in vitro by respiratory syncytial virus (RSV). For study of the interaction of eosinophils and RSV on the ultrastructural level, normodense eosinophils were purified from peripheral blood of healthy human volunteers. After incubation with RSV in the presence or absence of autologous serum, the eosinophils were examined with immunofluorescence microscopy employing an RSV-specific monoclonal antibody, and with transmission electron microscopy. After 2-h incubation in the presence of live RSV, 25.6 ± 12.9% of the eosinophils demonstrated positive fluorescence. This increased to 62.8 ± 8.9% when fresh autologous serum was added during incubation (P = 0.015). The effect was abolished when the serum was heat-treated, indicating Fc-receptor-independent enhancement of viral uptake by the cells. In transmission electron microscopy, virions were seen in phagocytic vacuoles at the periphery of the cells. Eosinophil activation characterized by piecemeal degranulation was evident. In conclusion, activation of eosinophils during RSV bronchiolitis probably occurs in part by direct interaction of the cells with the virus.

Original languageEnglish (US)
Pages (from-to)48-53
Number of pages6
JournalPediatric Allergy and Immunology
Volume7
Issue number1
StatePublished - Feb 1996

Fingerprint

Respiratory Syncytial Viruses
Eosinophils
Transmission Electron Microscopy
Serum
Bronchiolitis
Fc Receptors
Vacuoles
Fluorescence Microscopy
Cell Communication
Virion
Healthy Volunteers
Hot Temperature
Fluorescence
Monoclonal Antibodies
Viruses

Keywords

  • Electron microscopy
  • Eosinophils
  • Respiratory syncytial virus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pediatrics, Perinatology, and Child Health
  • Immunology

Cite this

An ultrastructural study of the interaction of human eosinophils with respiratory syncytial virus. / Kimpen, Jan L L; Garofalo, Roberto; Welliver, Robert C.; Fujihara, Keiji; Ogra, Peary L.

In: Pediatric Allergy and Immunology, Vol. 7, No. 1, 02.1996, p. 48-53.

Research output: Contribution to journalArticle

Kimpen, Jan L L ; Garofalo, Roberto ; Welliver, Robert C. ; Fujihara, Keiji ; Ogra, Peary L. / An ultrastructural study of the interaction of human eosinophils with respiratory syncytial virus. In: Pediatric Allergy and Immunology. 1996 ; Vol. 7, No. 1. pp. 48-53.
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