Analysis of a Yellow Fever Virus Isolated from a Fatal Case of Vaccine-Associated Human Encephalitis

A. D. Jennings, C. A. Gibson, B. R. Miller, J. H. Mathews, C. J. Mitchell, J. T. Roehrig, D. J. Wood, F. Taffs, B. K. Sil, S. N. Whitby, J. E. Whitby, T. P. Monath, P. D. Minor, P. G. Sanders, Alan Barrett

Research output: Contribution to journalArticle

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Abstract

The virulence of a yellow fever (YF) virus (P-16065) isolated from a fatal case of vaccine-associated viral encephalitis was investigated. P-16065 appeared identical to its parent vaccine virus (17D-204 USA, lot 6145) when examined with monoclonal antibodies except that YF wild type-specific MAb S24 recognized P-16065 but not 17D-204 USA 6145. Thus, a mutation of at least one epitope on the envelope (E) protein had occurred. Unlike 17D-204 USA 6145 and other 17D vaccine viruses, P-16065 was neuroinvasive and virulent for mice after intranasal inoculation, and neurovirulent for monkeys after intracerebral inoculation. The E protein of P-16065 differed from 17D-204 USA by two amino acids at positions 155 and 303. Changes at amino acid position 155 are found in other YF vaccine viruses that are not neurovirulent, and it is therefore postulated that the change at position 303 is involved in the alteration of the phenotype of P-16065 and may be important for virulence of YF virus.

Original languageEnglish (US)
Pages (from-to)512-518
Number of pages7
JournalJournal of Infectious Diseases
Volume169
Issue number3
StatePublished - Mar 1994

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Yellow fever virus
Encephalitis
Vaccines
Virulence
Yellow Fever Vaccine
Viral Encephalitis
Viruses
Yellow Fever
Amino Acids
Haplorhini
Epitopes
Proteins
Monoclonal Antibodies
Phenotype
Mutation

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Jennings, A. D., Gibson, C. A., Miller, B. R., Mathews, J. H., Mitchell, C. J., Roehrig, J. T., ... Barrett, A. (1994). Analysis of a Yellow Fever Virus Isolated from a Fatal Case of Vaccine-Associated Human Encephalitis. Journal of Infectious Diseases, 169(3), 512-518.

Analysis of a Yellow Fever Virus Isolated from a Fatal Case of Vaccine-Associated Human Encephalitis. / Jennings, A. D.; Gibson, C. A.; Miller, B. R.; Mathews, J. H.; Mitchell, C. J.; Roehrig, J. T.; Wood, D. J.; Taffs, F.; Sil, B. K.; Whitby, S. N.; Whitby, J. E.; Monath, T. P.; Minor, P. D.; Sanders, P. G.; Barrett, Alan.

In: Journal of Infectious Diseases, Vol. 169, No. 3, 03.1994, p. 512-518.

Research output: Contribution to journalArticle

Jennings, AD, Gibson, CA, Miller, BR, Mathews, JH, Mitchell, CJ, Roehrig, JT, Wood, DJ, Taffs, F, Sil, BK, Whitby, SN, Whitby, JE, Monath, TP, Minor, PD, Sanders, PG & Barrett, A 1994, 'Analysis of a Yellow Fever Virus Isolated from a Fatal Case of Vaccine-Associated Human Encephalitis', Journal of Infectious Diseases, vol. 169, no. 3, pp. 512-518.
Jennings AD, Gibson CA, Miller BR, Mathews JH, Mitchell CJ, Roehrig JT et al. Analysis of a Yellow Fever Virus Isolated from a Fatal Case of Vaccine-Associated Human Encephalitis. Journal of Infectious Diseases. 1994 Mar;169(3):512-518.
Jennings, A. D. ; Gibson, C. A. ; Miller, B. R. ; Mathews, J. H. ; Mitchell, C. J. ; Roehrig, J. T. ; Wood, D. J. ; Taffs, F. ; Sil, B. K. ; Whitby, S. N. ; Whitby, J. E. ; Monath, T. P. ; Minor, P. D. ; Sanders, P. G. ; Barrett, Alan. / Analysis of a Yellow Fever Virus Isolated from a Fatal Case of Vaccine-Associated Human Encephalitis. In: Journal of Infectious Diseases. 1994 ; Vol. 169, No. 3. pp. 512-518.
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AB - The virulence of a yellow fever (YF) virus (P-16065) isolated from a fatal case of vaccine-associated viral encephalitis was investigated. P-16065 appeared identical to its parent vaccine virus (17D-204 USA, lot 6145) when examined with monoclonal antibodies except that YF wild type-specific MAb S24 recognized P-16065 but not 17D-204 USA 6145. Thus, a mutation of at least one epitope on the envelope (E) protein had occurred. Unlike 17D-204 USA 6145 and other 17D vaccine viruses, P-16065 was neuroinvasive and virulent for mice after intranasal inoculation, and neurovirulent for monkeys after intracerebral inoculation. The E protein of P-16065 differed from 17D-204 USA by two amino acids at positions 155 and 303. Changes at amino acid position 155 are found in other YF vaccine viruses that are not neurovirulent, and it is therefore postulated that the change at position 303 is involved in the alteration of the phenotype of P-16065 and may be important for virulence of YF virus.

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