Analysis of free ACh and 5-HT in milk from four different species and their bioactivity on 5-HT3 and nACh receptors

Jose Luis Gallegos-Perez, Agenor Limon-Ruiz, Jorge M. Reyes-Ruiz, Ali S. Alshanqeeti, Mohammad A. Aljohi, Ricardo Miledi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Milk is one of the most beneficial aliments and is highly recommended in normal conditions; however, in certain disorders, like irritable bowel syndrome, cow milk and dairy products worsen the gastric symptoms and their use is not recommended. Among the most recognized milk-induced gatrointestinal symptoms are abdominal pain, nausea and vomiting, which are processes controlled by cholinergic and serotonergic transmission. Whether the presence of bioavailable ACh and 5-HT in milk may contribute to normal peristalsis, or to the developing of these symptoms, is not known. In this work we attempt to determine whether the content of free ACh and 5-HT is of physiological significance in milk from four different species: cow (bovine), goat, camel and human. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to identify and quantify free ACh and 5-HT in milk, and activation of the serotonergic and cholinergic ionotropic receptors was investigated using electrophysiological experiments. Our principal hypothesis was that milk from these four species had sufficient free ACh and 5-HT to activate their correspondent receptors expressed in a heterologous system. Our results showed a more complex picture, in which free ACh and 5-HT and their ability to activate cholinergic and serotonergic receptors are not correlated. This work is a first step to elucidate whether 5-HT and ACh, at the concentrations present in the milk, can be associated to a direct function in the GI. This journal is

Original languageEnglish (US)
Pages (from-to)1489-1494
Number of pages6
JournalFood and Function
Volume5
Issue number7
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Receptors, Serotonin, 5-HT3
serotonin
Serotonin
Milk
milk
receptors
signs and symptoms (animals and humans)
cholinergic agents
Cholinergic Receptors
dairy products
peristalsis
Peristalsis
Camelus
nausea
cholinergic receptors
Dairy Products
Irritable Bowel Syndrome
camels
vomiting
Tandem Mass Spectrometry

ASJC Scopus subject areas

  • Food Science

Cite this

Analysis of free ACh and 5-HT in milk from four different species and their bioactivity on 5-HT3 and nACh receptors. / Gallegos-Perez, Jose Luis; Limon-Ruiz, Agenor; Reyes-Ruiz, Jorge M.; Alshanqeeti, Ali S.; Aljohi, Mohammad A.; Miledi, Ricardo.

In: Food and Function, Vol. 5, No. 7, 01.01.2014, p. 1489-1494.

Research output: Contribution to journalArticle

Gallegos-Perez, Jose Luis ; Limon-Ruiz, Agenor ; Reyes-Ruiz, Jorge M. ; Alshanqeeti, Ali S. ; Aljohi, Mohammad A. ; Miledi, Ricardo. / Analysis of free ACh and 5-HT in milk from four different species and their bioactivity on 5-HT3 and nACh receptors. In: Food and Function. 2014 ; Vol. 5, No. 7. pp. 1489-1494.
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