Analysis of multiple molecular changes in human endocrine tumours

I. Arany, P. Rady, B. M. Evers, S. K. Tyring, Courtney Townsend

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To define the molecular changes occurring in endocrine tumours, we have analysed three human endocrine tumours established in our laboratory: BON, a functioning carcinoid tumour from the pancreas; SIM, a nonfunctioning carcinoid of the ileum; and STAN, a pheochromocytoma. A homozygous point mutation of the N-ras gene was identified at codon 61 in BON cells in conjunction with overexpression of N-ras mRNA and protein. BON cells also exhibited increased expression of c-myc and cdc2 kinase mRNA and protein; TGF-β1, p53 and retinoblastoma (RB) mRNA and protein levels were decreased. In addition, increased expression of the mdm2 oncogene and both the truncated and the wild-type RB protein were noted in BON. SIM cells exhibited moderately increased N-ras and c-myc mRNA levels along with decreased levels of RB mRNA and protein. Similar to BON and SIM, analysis of STAN showed increased N-ras and c-myc levels. Our data show multiple molecular changes in the three human endocrine tumours with the BON cell line exhibiting the most dramatic changes. Furthermore, our data suggest the existence of different molecular pathways in the pathogenesis of endocrine tumours. These cell lines will provide unique in vitro models to further analyse the significance of these molecular alterations.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalSurgical Oncology
Volume3
Issue number3
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Retinoblastoma Protein
Messenger RNA
Carcinoid Tumor
Neoplasms
ras Proteins
Cell Line
ras Genes
Pheochromocytoma
Oncogenes
Ileum
Point Mutation
Codon
Protein Kinases
Pancreas

Keywords

  • cytokines
  • endocrine tumours
  • oncogenes
  • tumour suppressor genes

ASJC Scopus subject areas

  • Oncology
  • Surgery

Cite this

Analysis of multiple molecular changes in human endocrine tumours. / Arany, I.; Rady, P.; Evers, B. M.; Tyring, S. K.; Townsend, Courtney.

In: Surgical Oncology, Vol. 3, No. 3, 1994, p. 153-159.

Research output: Contribution to journalArticle

Arany, I. ; Rady, P. ; Evers, B. M. ; Tyring, S. K. ; Townsend, Courtney. / Analysis of multiple molecular changes in human endocrine tumours. In: Surgical Oncology. 1994 ; Vol. 3, No. 3. pp. 153-159.
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