TY - JOUR
T1 - Analysis of nif and regulatory genes in acetobacter diazotrophicus
AU - Sevilla, M.
AU - Meletzus, D.
AU - Teixeira, K.
AU - Lee, S.
AU - Nutakki, A.
AU - Baldani, I.
AU - Kennedy, C.
N1 - Funding Information:
Acknowledgements-We thank NSF and USDA (U.S.A.). DFG (Germany) and CNpQ (Brazil) for funding support. We are also grateful to Johanna Dobereiner for her seminal discovery of Acelobacter diazotrophicus and her enthusiastic encouragement.
PY - 1997
Y1 - 1997
N2 - The focus of our collaborative research program is the identification and characterization of nif and related regulatory genes of Acetobacter diazotrophicus in order to understand what factors influence nitrogen fixation in this unique diazotroph. To date, the following genes have been isolated from genomic libraries and are being analyzed: nifHDK, nifA, nifB, nifV, nifE and ntrBC. In addition, Nif- mutants have been constructed by insertional mutagenesis. These mutants are currently being used in inoculation experiments of sterile sugarcane plants to determine whether nitrogen fixed by A. diazotrophicus is significant for plant nutrition.
AB - The focus of our collaborative research program is the identification and characterization of nif and related regulatory genes of Acetobacter diazotrophicus in order to understand what factors influence nitrogen fixation in this unique diazotroph. To date, the following genes have been isolated from genomic libraries and are being analyzed: nifHDK, nifA, nifB, nifV, nifE and ntrBC. In addition, Nif- mutants have been constructed by insertional mutagenesis. These mutants are currently being used in inoculation experiments of sterile sugarcane plants to determine whether nitrogen fixed by A. diazotrophicus is significant for plant nutrition.
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U2 - 10.1016/S0038-0717(96)00210-6
DO - 10.1016/S0038-0717(96)00210-6
M3 - Article
AN - SCOPUS:0030878362
SN - 0038-0717
VL - 29
SP - 871
EP - 874
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
IS - 5-6
ER -