Analysis of nif and regulatory genes in acetobacter diazotrophicus

M. Sevilla, D. Meletzus, K. Teixeira, S. Lee, A. Nutakki, I. Baldani, C. Kennedy

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


The focus of our collaborative research program is the identification and characterization of nif and related regulatory genes of Acetobacter diazotrophicus in order to understand what factors influence nitrogen fixation in this unique diazotroph. To date, the following genes have been isolated from genomic libraries and are being analyzed: nifHDK, nifA, nifB, nifV, nifE and ntrBC. In addition, Nif- mutants have been constructed by insertional mutagenesis. These mutants are currently being used in inoculation experiments of sterile sugarcane plants to determine whether nitrogen fixed by A. diazotrophicus is significant for plant nutrition.

Original languageEnglish (US)
Pages (from-to)871-874
Number of pages4
JournalSoil Biology and Biochemistry
Issue number5-6
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Soil Science


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