Analysis of nif and regulatory genes in acetobacter diazotrophicus

M. Sevilla; D. Meletzus; K. Teixeira; S. Lee; A. Nutakki; I. Baldani; C. Kennedy

doi:10.1016/S0038-0717(96)00210-6

Analysis of nif and regulatory genes in acetobacter diazotrophicus

M. Sevilla
, D. Meletzus
, K. Teixeira
, S. Lee
, A. Nutakki
, I. Baldani
, C. Kennedy

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The focus of our collaborative research program is the identification and characterization of nif and related regulatory genes of Acetobacter diazotrophicus in order to understand what factors influence nitrogen fixation in this unique diazotroph. To date, the following genes have been isolated from genomic libraries and are being analyzed: nifHDK, nifA, nifB, nifV, nifE and ntrBC. In addition, Nif^- mutants have been constructed by insertional mutagenesis. These mutants are currently being used in inoculation experiments of sterile sugarcane plants to determine whether nitrogen fixed by A. diazotrophicus is significant for plant nutrition.

Original language	English (US)
Pages (from-to)	871-874
Number of pages	4
Journal	Soil Biology and Biochemistry
Volume	29
Issue number	5-6
DOIs	https://doi.org/10.1016/S0038-0717(96)00210-6
State	Published - 1997
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Soil Science

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10.1016/S0038-0717(96)00210-6

Cite this

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title = "Analysis of nif and regulatory genes in acetobacter diazotrophicus",

abstract = "The focus of our collaborative research program is the identification and characterization of nif and related regulatory genes of Acetobacter diazotrophicus in order to understand what factors influence nitrogen fixation in this unique diazotroph. To date, the following genes have been isolated from genomic libraries and are being analyzed: nifHDK, nifA, nifB, nifV, nifE and ntrBC. In addition, Nif- mutants have been constructed by insertional mutagenesis. These mutants are currently being used in inoculation experiments of sterile sugarcane plants to determine whether nitrogen fixed by A. diazotrophicus is significant for plant nutrition.",

author = "M. Sevilla and D. Meletzus and K. Teixeira and S. Lee and A. Nutakki and I. Baldani and C. Kennedy",

note = "Funding Information: Acknowledgements-We thank NSF and USDA (U.S.A.). DFG (Germany) and CNpQ (Brazil) for funding support. We are also grateful to Johanna Dobereiner for her seminal discovery of Acelobacter diazotrophicus and her enthusiastic encouragement.",

year = "1997",

doi = "10.1016/S0038-0717(96)00210-6",

language = "English (US)",

volume = "29",

pages = "871--874",

journal = "Soil Biology and Biochemistry",

issn = "0038-0717",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Analysis of nif and regulatory genes in acetobacter diazotrophicus

AU - Sevilla, M.

AU - Meletzus, D.

AU - Teixeira, K.

AU - Lee, S.

AU - Nutakki, A.

AU - Baldani, I.

AU - Kennedy, C.

N1 - Funding Information: Acknowledgements-We thank NSF and USDA (U.S.A.). DFG (Germany) and CNpQ (Brazil) for funding support. We are also grateful to Johanna Dobereiner for her seminal discovery of Acelobacter diazotrophicus and her enthusiastic encouragement.

PY - 1997

Y1 - 1997

N2 - The focus of our collaborative research program is the identification and characterization of nif and related regulatory genes of Acetobacter diazotrophicus in order to understand what factors influence nitrogen fixation in this unique diazotroph. To date, the following genes have been isolated from genomic libraries and are being analyzed: nifHDK, nifA, nifB, nifV, nifE and ntrBC. In addition, Nif- mutants have been constructed by insertional mutagenesis. These mutants are currently being used in inoculation experiments of sterile sugarcane plants to determine whether nitrogen fixed by A. diazotrophicus is significant for plant nutrition.

AB - The focus of our collaborative research program is the identification and characterization of nif and related regulatory genes of Acetobacter diazotrophicus in order to understand what factors influence nitrogen fixation in this unique diazotroph. To date, the following genes have been isolated from genomic libraries and are being analyzed: nifHDK, nifA, nifB, nifV, nifE and ntrBC. In addition, Nif- mutants have been constructed by insertional mutagenesis. These mutants are currently being used in inoculation experiments of sterile sugarcane plants to determine whether nitrogen fixed by A. diazotrophicus is significant for plant nutrition.

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U2 - 10.1016/S0038-0717(96)00210-6

DO - 10.1016/S0038-0717(96)00210-6

M3 - Article

AN - SCOPUS:0030878362

SN - 0038-0717

VL - 29

SP - 871

EP - 874

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

IS - 5-6

ER -

Analysis of nif and regulatory genes in acetobacter diazotrophicus

Abstract

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